Search

In-house immunoaffinity column for aflatoxins (AFs) detection is firstly developed by Kasetsart University since 2011 i.e. the first IAC prototype, KU-AF01. Detection of aflatoxin B1 (AFB1) up to 200 ng is considered significantly efficient, but the production cost is considered high. The high cost was due to the cost of monoclonal antibody specific to aflatoxin and its supporting materials i.e. CNBr-activated sepharose 4B. Therefore, this study was aimed to improve the efficiency of in-house immunoaffinity colums by replacing CNBr-activated sepharose 4B with other supporting materials and to lower the ratio of antibody to supporting materials.  Response surface methodology (RSM) was applied to determine the optimum alternative supporting materials and the ratio. Results revealed that when compared with CNBr-activated sepharose 4B, all materials tested could recover higher than 80% AFB1, except the activated CH sepharose 4B. Results also indicated that the optimum ratio was 0.4 mg anti-aflatoxin monoclonal antibody to 204 µL CNBr-activated sepharose 4B. CNBr-activated sepharose 4B was considered to provide the best precision in recovering AFB1. KU-AF02 increased the ability to detect AFB1 to 500 ppb. The recovery of AFs in the reference materials using KU-AF02 was more than 96% successful, with HorRat value range of 0.34 – 0.76. The reference materials used were 2 levels of AFs in peanuts, i.e. 47.68 and 72.14 ng/g. In terms of quality control in IAC production, there were no significant differences among the 5 batches produced (p > 0.05). KU-AF02 demonstrated stable and constant percent recovery at 4 oC for up to 12 months (tested with standard 200 ppb AFB1). This study indicated that KU-AF02 could lower the ratio of supporting materials to the antibody and that KU-AF02 has high stability and has good ability for cleaning up AFs. Therefore, KU-AF02 is recommended to be used as an in-house immunoaffinity column for aflatoxin detection in Thailand.

Aflatoxins (AFs) and aflatoxin B1 (AFB1) intake through consumption of peanuts and peanut products were estimated from 60 samples. The samples were collected from 2 retail and 2 wholesale markets in Bangkok during November 2013–January 2014. The results showed that 80% of raw peanut samples were contaminated with AFs while 100% of roasted and ground peanuts samples were contaminated. The highest concentration of AFs was found in ground peanuts at 362.48 ng g−1 resulting in the highest mean concentration (68.22 ng g−1). According to a food consumption database, the average intake of AFs was estimated at 0.49, 0.40 and 2.13 ng/kg bw/day for raw, roasted and ground peanuts, respectively. The potential risk for cancer was estimated at 0.01–0.12 cancer/year/100,000 persons. The results suggest that the current situation of aflatoxins contamination in peanuts and peanut products (especially in ground peanuts) has an adverse effect on the health of the Thai population.