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Soil organic carbon (SOC) plays a crucial role in arid zones, while land-use change could lead to changes in the balance of SOC. Therefore, the aim of this study was to determine the impact of land-use change on the temporal and spatial variability in SOC at the county scale. A semi-arid zone (Aohan County, Northeast China) covering a surface of 3800 km2 was selected for this study. SOC data for 65 and 182 soil samples from 1985 and 2021, respectively, were collected for major land-use types (forestland, farmland, grassland, and sandy land) across Aohan County to a depth of 20 cm. The impacts of different land-use types and land-use changes on SOC were evaluated. The results showed that land-use change enhanced the spatial variability in SOC over the last 36 years. The mean SOC in 2021 (7.49 g kg−1) was significantly higher than that in 1985 (6.91 g kg−1). Converting sandy land into grassland and farmland into forest or grassland would lead to significant accumulation of SOC, while the depletion of SOC occurred after grassland afforestation. The balance between aboveground biomass inputs and SOC decomposition was the determining reason that affected the accumulation of SOC. Vegetation restoration due to land-use change could alter both soil texture and the C/N ratio and could have positive effects on ecosystem recovery. [ABSTRACT FROM AUTHOR]