1. Adjuvant effect of mushroom glucan and bovine lactoferrin upon Aeromonas hydrophila vaccination in catla, Catla catla (Hamilton).
- Author
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Kamilya D, Maiti TK, Joardar SN, and Mal BC
- Subjects
- Animals, Antibodies, Bacterial blood, Antigens, Bacterial immunology, Cattle, Cyprinidae microbiology, Fish Diseases immunology, Fish Diseases microbiology, Fish Diseases mortality, Glucans administration & dosage, Glucans immunology, Gram-Negative Bacterial Infections immunology, Gram-Negative Bacterial Infections mortality, Gram-Negative Bacterial Infections prevention & control, Lactoferrin administration & dosage, Lactoferrin immunology, Leukocytes immunology, Macrophage-Activating Factors analysis, Macrophage-Activating Factors biosynthesis, Pleurotus chemistry, Vaccination veterinary, Vaccines, Inactivated administration & dosage, Vaccines, Inactivated pharmacology, Adjuvants, Immunologic pharmacology, Aeromonas hydrophila immunology, Bacterial Vaccines, Cyprinidae immunology, Fish Diseases prevention & control, Gram-Negative Bacterial Infections veterinary
- Abstract
Mushroom glucan and bovine lactoferrin (Lf), known for their immunostimulatory potential, were used as adjuvant in conjunction with a formalin-killed Aeromonas hydrophila vaccine in catla, Catla catla. In vitro antigen-specific responsiveness of catla leucocytes and protective responses against experimental challenge with homologous antigen were monitored following immunization. Antigen-specific proliferation, 'macrophage activating factor' (MAF) production and antibody production were significantly higher in fish injected with glucan adjuvanted vaccine. Lf adjuvanted preparations showed a weak proliferative response and MAF production, although the antibody production was significantly higher than the controls. A good degree of protection was achieved with the glucan adjuvanted vaccine. However, in spite of producing significant anti-A. hydrophila antibody, Lf adjuvanted vaccine did not confer any protection following challenge with A. hydrophila. The potential of adjuvanticity of mushroom glucan and bovine Lf in intraperitoneal vaccination is discussed.
- Published
- 2006
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