1. A novel TTX-producing Aeromonas isolated from the ovary of Takifugu obscurus.
- Author
-
Yang G, Xu J, Liang S, Ren D, Yan X, and Bao B
- Subjects
- Aeromonas genetics, Animals, Chromatography, High Pressure Liquid, DNA, Ribosomal genetics, Enzyme-Linked Immunosorbent Assay, Female, Mass Spectrometry, Aeromonas metabolism, Tetraodontiformes microbiology, Tetrodotoxin biosynthesis
- Abstract
Puffer fish (Takifugu obscurus) from the Yangtze River of China were screened for tetrodotoxin-producing bacteria. An Aeromonas strain was isolated from the ovary of the puffer fish and was shown to produce tetrodotoxin; this strain was denoted Ne-1. The identity of tetrodotoxin produced by strain Ne-1 was confirmed by mouse bioassay, high performance liquid chromatography coupled with mass chromatography (LC-MS), and ELISA. Strain Ne-1 was characterized morphologically, biochemically, and by 16S rDNA phylogenetic analysis; these analyses suggested that strain Ne-1 is closely related to Aeromonas molluscorum. Given that strain Ne-1 was isolated from the ovary of T. obscurus, we propose that the TTX-producing Aeromonas sp. is a parasite or symbiotic bacterium rather than a sample contaminant. Collectively, our studies suggest that Aeromonas sp. strain Ne-1 produces tetrodotoxin in T. obscurus., (Copyright 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2010
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