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Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV. [ABSTRACT FROM AUTHOR]

Aedes aegypti and Aedes albopictus are predominantly daytime-feeding mosquito species that breed in urban settlements and transmit viral and nematode diseases. Females can feed on a broad range of vertebrate hosts for oogenesis. Research on host-feeding behavior and patterns is important to better understand the transmission cycles of most mosquito-borne diseases and to develop better protection measures. This study investigated the host feeding patterns of Ae. aegypti and Ae. albopictus through both artificial blood feeding and natural feeding techniques and assessed the effect of these methods on mosquito fecundity. Laboratory colonies of both mosquitoes fed on blood from five different hosts (bird, mouse, human, sheep and cow). The artificial blood feeding system consisted of a glass condenser with blood attached to a water bath with warm water circulation and insects fed through a stretched Parafilm membrane over the bottom of the condenser. Natural feeding experiments were performed with paper cups that contained the mosquitoes and were placed over the host's skin for one hour. In the natural feeding method, Ae. aegypti females mostly fed on humans, while in the artificial blood feeding method, they fed on birds. Both human and bird blood sources yielded the highest number of eggs, larvae and pupae for Ae. aegypti. Aedes albopictus preferred humans in the natural feeding method and mice in the artificial blood feeding method. Similarly, egg, larvae and pupae productivity from humans and mice were also high for Ae. albopictus. Both species preferred the artificial feeding method. Our findings show that different blood meal sources and methods affect mosquito fecundity. Such laboratory studies on mosquito feeding patterns can reveal the feeding preference of important vectors and provide vital information for understanding vector and host interactions. [ABSTRACT FROM AUTHOR]

Several dengue epidemics recently occurred in La Reunion, an island harboring two dengue viruses (DVs) vectors: Aedes albopictus, and Ae. aegypti, the former being the main local DV vector. Aedes aegypti shows a peculiar ecology, compared to other tropical populations of the same species. This study aimed to provide researchers and public-health users with locally validated oviposition traps (ovitraps) to monitor Aedes populations. A field experiment was performed in Saint-Joseph to assess the effect of different settings on the detection probability and apparent density of Aedes mosquitoes. Black plastic ovitraps were identified as the best choice. Vacoa trees (Pandanus utilis) were the only observed breeding sites for Ae. aegypti, shared with Ae. albopictus. They were the experimental units in a Latin square design with three factors: trap position in the trees (ground vs canopy), oviposition surface in the trap (blotting paper vs. vacoa leaf), and addition of organic matter to the trap water. The latter factor was found unimportant. On the ground, Ae. aegypti eggs were only found with vacoa leaves as the oviposition surface. Their detection and apparent density increased when ovitraps were located in the tree canopy. The main factor for Ae. albopictus was the oviposition surface, with a preference for blotting paper. In all trap settings, their detection was close to 100%. Larval survival was lower for a high egg density, combined with blotting paper as the oviposition surface. When monitoring mixed Aedes populations in La Reunion, we recommend using black plastic ovitraps, placed at 1.50-to-2.00-m high in vacoa trees, with vacoa leaves as the oviposition surface. [ABSTRACT FROM AUTHOR]

Background: Dengue, a mosquito-borne viral disease, has occurred in many cities in China, and it tends to spread to higher latitudes (Huang et al., 2023). Xi'an, situated in central-west China, has witnessed an increase imported cases in the past few years, raising concerns of local dengue transmission. It is crucial to investigate the population density of Aedes albopictus and its insecticides resistance to enhance early warning of dengue fever. Methods: Eight sampling sites in eight counties (YT, BL, WY, CH, YL, LN, LT, ZZ) of Xi'an city were surveyed by larval dipping and human-baited double net trap biweekly from June 2021 to September 2022. The Breteau Index (BI, number of positive containers per 100 houses) and Container Index (CI, the percentage of containers containing larvae or pupae) were used to assess larval density, and the human-baited double net trap (HDN, the number of Ae. albopictus females collected per person per hour) to indicate human bating rate (HBR). Meanwhile, the association between the meteorological factors and mosquito density was analyzed. The Ae. albopictus adult insecticides resistance was evaluated by the World Health Organization (WHO) standard resistance bioassay. Adult females were exposed to insecticide-impregnated paper for 1 h, then transferred to the recovery tube, and mortality rate was calculated after 24 h. According to the Implementation Plan for National Vector Surveillance (2016), resistance status was classified into three levels based on mortality: <80% mortality as resistant, between 80% and 98% mortality as possibly resistant, and >98% mortality as sensitive. Results: From June 2021 to September 2022, a total of 1,065 houses were surveyed for water holding containers, and 99 of 430 water holding containers were checked to be positive for Ae. albopictus larvae and pupae. A total of 1,048 Ae. albopictus females were collected. The average BI, CI and HBR were 10.39, 21.41, and 11.20 female/man/hour in 2021 and 8.86, 20.86, and 11.63 f/m/h in 2022, respectively. The findings showed that the BI exceeded 5 in most months and reached above 20 in specific months. The CI varied in different months and monitoring sites, with the highest CI in August 2021 and July 2022. The discarded tires had the highest positivity rate, with up to 40.32% testing positive for Ae. albopictus larvae. The monthly average temperature showed a positive correlation with CI (r = 0.77), and the monthly BI was positively associated with CI (r = 0.93). The BI, CI, and HBR were significantly higher in the rainy season than other seasons. The bioassay results showed that the mortality rate of Ae. albopictus at the YT monitoring site was 76.92%, indicating resistance to deltamethrin. The mortality rate of Ae. Albopictus at BL, WY, CH, YL, LN, LT, and ZZ sampling sites were varying from 81.25%∼100%, suggesting possibly resistant or still sensitive to beta-cypermethrin, alpha-cypermethrin, malathion, chlorpyrifos, and propoxur. [ABSTRACT FROM AUTHOR]

After mating, female mosquitoes need a blood meal to promote the reproductive process. When mosquitoes bite infected people and animals, they become infected with germs such as viruses and parasites. Mosquitoes rely on many cues for host selection and localization, among which the trace chemical cues emitted by the host into the environment are considered to be the most important, and the sense of smell is the main way to perceive these trace chemical cues. However, the current understanding of the olfactory mechanism is not enough to meet the needs of mosquito control. Unlike previous studies that focused on the olfactory receptor recognition spectrum to reveal the olfactory mechanism of mosquito host localization. In this paper, based on the observation that mosquitoes with incomplete antennae still can locate the host and complete blood feeding in the laboratory, we proposed that there may be some protection or compensation mechanism in the 13 segments of antennae flagella, and only when the antennae are missing to a certain threshold will it affect the mosquito's ability to locate the host. Through rational-designed behavioral experiments, we found that the 6th and 7th flagellomeres on the Aedes albopictus antenna are important in the olfactory detection of host searching. This study preliminarily screened antennal segments important for host localization of Ae. albopictus, and provided a reference for subsequent cell biology and molecular biology studies on these segments. Meanwhile, the morphology and distribution of sensilla on each antenna flagellomere were also analyzed and discussed in this paper. [ABSTRACT FROM AUTHOR]

As a widespread vector of disease with an expanding range, the mosquito Aedes albopictus Skuse (Diptera: Culicidae) is a high priority for research and management. A. albopictus has a complex life history with aquatic egg, larval and pupal stages, and a terrestrial adult stage. This requires targeted management strategies for each life stage, coordinated across time and space. Population genetics can aid in A. albopictus control by evaluating patterns of genetic diversity and dispersal. However, how life stage impacts population genetic characteristics is unknown. We examined whether patterns of A. albopictus genetic diversity and differentiation changed with life stage at a spatial scale relevant to management efforts. We first conducted a literature review of field‐caught A. albopictus population genetic papers and identified 101 peer‐reviewed publications, none of which compared results between life stages. Our study uniquely examines population genomic patterns of egg and adult A. albopictus at five sites in Wake County, North Carolina, USA, using 8425 single nucleotide polymorphisms. We found that the level of genetic diversity and connectivity between sites varied between adults and eggs. This warrants further study and is critical for research aimed at informing local management. [ABSTRACT FROM AUTHOR]

Chemical control is widely adopted method in mosquito control that emphasizes on the management of the adult and larval populations using various insecticides. Present study was carried out in the year 2015 to use chemicals which were currently being utilized in mosquito control program against prevalent mosquito species (Culex and Aedes spp.) collected from different ecological zones of Punjab (Pakistan) (i.e. Faisalabad, Lahore and Rawalpindi) formed as a result of dengue prevalence to evaluate their efficacy by performing adult bioassay using standard WHO protocols. Results revealed that among three pyrethroids used as adulticides, alpha-cypermethrin 10% SC showed greater residual efficacy and percent mortality on various substrates/surfaces like cement, mud, plaster, wood and filter paper followed by lambda-cyhalothrin 10% CS and deltamethrin 5WP in a period of 20 weeks. Response of these chemicals was greater on filter paper and cement and less on wood and mud. Data collected on these parameters was subjected to SPSS and Minitab software for drawing inferences. [ABSTRACT FROM AUTHOR]

Background: Dengue is a serious public health problem worldwide, including Panama. During the last years, the number of dengue cases has increased. This may be due to the presence of mosquito populations resistant to insecticides. The aim of this study was to characterize the resistance status, its enzymatic mechanisms and Kdr mutations in wild populations of Aedes aegypti and Aedes albopictus. Methods: Standard WHO bioassays were performed using insecticide-treated filter papers to determine resistance in populations Ae. aegypti and Ae. albopictus to pyrethroids insecticides, organophosphates, to the carbamate propoxur and to the organochlorine DDT. Biochemical assays were conducted to detect metabolic resistance mechanisms and real-time PCR was performed to determine the frequencies of the Kdr mutations Val1016IIe and F1534C. Results: The strains Ae. aegypti El Coco showed confirmed resistance to deltamethrin (78.5% mortality) and lambda-cyhalothrin (81%), Aguadulce to deltamethrin (79.3%), David to deltamethrin (74.8%) and lambda-cyhalothrin (87.5%) and Puerto Armuelles to permethrin (83%). Aedes aegypti El Empalme showed confirmed resistance to pirimiphos-methyl (62.3% mortality), chlorpyrifos-methyl (55.5%) and propoxur (85.3%). All strains of Ae. albopictus showed possible resistance to PYs and five strains to DDT. Only Ae. albopictus Canto del Llano showed confirmed resistance to pirimiphos-methyl (70% mortality) and malathion (62%). Esterase activity was variable across sites with the most frequent expression of α-EST compared to β-EST in Ae. aegypti populations. In Ae. Albopictus, the expressed enzymes were β-EST and MFOs. Through ANOVA, significant differences were established in the levels of enzymatic activity of α- and β-EST, MFOs and GST, with p < 0.001 in the Ae. aegypti and Ae. albopictus. The Kdr Val1016IIe mutation was detected in Ae. aegypti Aguadulce, El Coco and David. The odds ratio for the Val1016Ile mutation ranged from 0.8 to 20.8 in resistant mosquitoes, indicating the association between pyrethroid phenotypic resistance and the kdr mutation. Conclusion: The presence of a varied and generalized resistance, enzymatic mechanisms and the Val1016IIe mutation may be associated with the intensive use and possibly misuse of the different insecticides applied to control Aedes populations. These results highlight the need to develop a program for resistance management. Also, alternative approaches to mosquito control that do not involve insecticides should be explored. [ABSTRACT FROM AUTHOR]

Simple Summary: The role of mosquito eggs in disease transmission is not well understood, despite reports of virus vertical transmission and their ability to survive dry periods in a dormant stage. Additionally, field evidence shows that new generations of mosquitoes often reappear weeks or months later in certain city areas. This may be because both species follow an "R strategy" reproduction pattern, laying many eggs to ensure survival in a harsh environment. This study was conducted in southern Mexico in 2023 and focused on the egg-laying activity of Ae. aegypti and Ae. albopictus mosquitoes during the dry inter-epidemic season. Ovitraps were used in the backyards of the houses to identify egg species, and further larval containers were surveyed. Most of the larval habitats were containers smaller than 1 L, commonly not treated as they were considered trash. Even though homeowners volunteer to remove them during government cleaning community-based campaigns, city waste disposal trucks transporting these containers do not take any measures to destroy the tiny eggs. We conclude with the urgent need to develop an egg-killing treatment, which could significantly enhance vector reduction and achieve a total stage strategy within the Integrated Vector Control Programs. Although integrated management and control programs implement intense control measures for adult, pupal, larval, and breeding sites during outbreaks, there is a lack of studies to understand the role of the vector egg stage in disease dynamics. This study aimed to assess the dry season quiescent Aedes aegypti and Aedes albopictus egg populations in houses and backyards in Tapachula, southern Mexico. Two hundred and fifty ovitraps were placed in 125 homes in the Las Americas neighborhood. A total of 7290 eggs were collected from 211 (84.4%) ovitraps. Only 5667 (77.7%) hatched under insectary water immersion and food supply conditions, with 4031 (71.1%) identified as Ae. aegypti, and 1636 (28.8%) as Ae. albopictus, respectively. The remaining 1623 (22.3%) did not hatch due to Delayed Hatching and/or quiescence tropical stage. Eighty-three larval containers were sampled with desiccated eggs during the dry season; most of them were described as trash waste because larvicides are only used for larger containers of 5–10 L. Evolutionary characteristics for the two species including partial egg hatching, ambient-regulated quiescence, the ability of the embryo to survive for a more extended period intra-seasonally, the egg sticking to inner container walls, demands urgent operational research to achieve successful egg-proof larval container methods. [ABSTRACT FROM AUTHOR]

Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These diseases can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes and are known to produce an array of natural products with various activities including larvicidal activity. In this study, the effects of natural products produced by four Xenorhabdus and one Photorhabdus bacteria on the ovipositional behavior of Ae. albopictus mosquitoes were assessed. Utilizing a binary choice assay in insect cages, gravid female mosquitoes were presented with two oviposition cups containing water supplemented with varying concentrations of bacterial supernatants (50–1% concentrations) versus control medium. After 72 h, the eggs deposited on filter papers were counted. The oviposition attractant index (OAI) feature of the bacterial supernatant was evaluated using the number of eggs laid in the cups. Notably, all tested supernatants exhibited concentration-dependent deterrence of oviposition. Xenorhabdus cabanillasii displayed the strongest deterrent effect, inhibiting egg-laying at 50–5% concentrations (OAI: − 0.87 to − 0.35), followed by X. nematophila (50–10%, OAI: − 0.82 to − 0.52). Xenorhabdus szentirmaii, X. doucetiae, and P. kayaii showed significant deterrence at ≥ 20% concentrations. Using promoter exchange mutants generated by the easyPACId approach, fabclavine from X. szentirmaii was identified as the bioactive compound with evident deterrent effects. Such deterrents targeting egg-laying could be valuable for controlling populations by disrupting their breeding in suitable habitats. [ABSTRACT FROM AUTHOR]

Chemical control is widely adopted method in mosquito control that emphasizes on the management of the adult and larval populations using various insecticides. Present study was carried out in the year 2015 to use chemicals which were currently being utilized in mosquito control program against prevalent mosquito species (Culex and Aedes spp.) collected from different ecological zones of Punjab (Pakistan) (i.e. Faisalabad, Lahore and Rawalpindi) formed as a result of dengue prevalence to evaluate their efficacy by performing adult bioassay using standard WHO protocols. Results revealed that among three pyrethroids used as adulticides, alpha-cypermethrin 10% SC showed greater residual efficacy and percent mortality on various substrates/surfaces like cement, mud, plaster, wood and filter paper followed by lambda-cyhalothrin 10% CS and deltamethrin 5WP in a period of 20 weeks. Response of these chemicals was greater on filter paper and cement and less on wood and mud. Data collected on these parameters was subjected to SPSS and Minitab software for drawing inferences. [ABSTRACT FROM AUTHOR]

Recurring seasonal changes can lead to the evolution of phenological cues. For example, many arthropods undergo photoperiodic diapause, a programmed developmental arrest induced by short autumnal day length. The selective mechanisms that determine the timing of autumnal diapause initiation have not been empirically identified. We quantified latitudinal clines in genetically determined diapause timing of an invasive mosquito, Aedes albopictus, on two continents. We show that variation in diapause timing within and between continents is explained by a novel application of a growing degree day (GDD) model that delineates a location‐specific deadline after which it is not possible to complete an additional full life cycle. GDD models are widely used to predict spring phenology by modelling growth and development as physiological responses to ambient temperatures. Our results show that the energy accumulation dynamics represented by GDD models have also led to the evolution of an anticipatory life‐history cue in autumn. [ABSTRACT FROM AUTHOR]

This paper summarises the lessons learnt in dengue epidemiology, risk factors, and prevention in Singapore over the last half a century, during which Singapore evolved from a city of 1.9 million people to a highly urban globalised city-state with a population of 5.6 million. Set in a tropical climate, urbanisation among green foliage has created ideal conditions for the proliferation of Aedes aegypti and Aedes albopictus, the mosquito vectors that transmit dengue. A vector control programme, largely for malaria, was initiated as early as 1921, but it was only in 1966 that the Vector Control Unit (VCU) was established to additionally tackle dengue haemorrhagic fever (DHF) that was first documented in the 1960s. Centred on source reduction and public education, and based on research into the bionomics and ecology of the vectors, the programme successfully reduced the Aedes House Index (HI) from 48% in 1966 to <5% in the 1970s. Further enhancement of the programme, including through legislation, suppressed the Aedes HI to around 1% from the 1990s. The current programme is characterised by 4 key features: (i) proactive inter-epidemic surveillance and control that is stepped up during outbreaks; (ii) risk-based prevention and intervention strategies based on advanced data analytics; (iii) coordinated inter-sectoral cooperation between the public, private, and people sectors; and (iv) evidence-based adoption of new tools and strategies. Dengue seroprevalence and force of infection (FOI) among residents have substantially and continuously declined over the 5 decades. This is consistent with the observation that dengue incidence has been delayed to adulthood, with severity highest among the elderly. Paradoxically, the number of reported dengue cases and outbreaks has increased since the 1990s with record-breaking epidemics. We propose that Singapore's increased vulnerability to outbreaks is due to low levels of immunity in the population, constant introduction of new viral variants, expanding urban centres, and increasing human density. The growing magnitude of reported outbreaks could also be attributed to improved diagnostics and surveillance, which at least partially explains the discord between rising trend in cases and the continuous reduction in dengue seroprevalence. Changing global and local landscapes, including climate change, increasing urbanisation and global physical connectivity are expected to make dengue control even more challenging. The adoption of new vector surveillance and control tools, such as the Gravitrap and Wolbachia technology, is important to impede the growing threat of dengue and other Aedes-borne diseases. Author summary: A densely populated, highly urban tropical city-state with long-established populations of Aedes aegypti and Ae. albopictus mosquitoes, plus travel and trade links to all corners of the world, Singapore is ideally suited for dengue transmission. Singapore's experience and rich surveillance data provide important insights for an increasingly large number of territories at risk of dengue epidemics. Decades of vector control efforts, focused on source reduction and surveillance, have successfully lowered the vector population, with accompanying reductions in the resident population's seroprevalence to dengue. We propose that Singapore's vulnerability to outbreaks is due to low levels of immunity in the population, constant introduction of viral variants, expanding urban centres, and increasing human density. The discord between the rising trend in reported cases and falling seroprevalence could be at least partly attributed to improved diagnostics and surveillance. Singapore's evidence-based vector-control programme, which involves strong partnership between the public and private sectors, will continue to adapt to future challenges in this space. [ABSTRACT FROM AUTHOR]

Aedesalbopictus (Diptera: Culicidae) is a mosquito from Asia that can transmit a variety of diseases. This paper aimed to explore the effects of temperature, relative humidity, and illumination on the entomological parameters related to the population growth of Aedes albopictus, and provide specific parameters for developing dynamic models of mosquito-borne infectious disease. We used artificial simulation lab experiments, and set 27 different meteorological conditions to observe and record mosquito's hatching time, emergence time, longevity of adult females, and oviposition amount. We then applied generalized additive model (GAM) and polynomial regression to formulate the effects of temperature, relative humidity, and illumination on the biological characteristics of Aedes albopictus. Our results showed that hatchability closely related to temperature and illumination. The immature stage and the survival time of adult female mosquitoes were associated with temperature and relative humidity. The oviposition rate related to temperature, relative humidity, and illumination. Under the control of relative humidity and illumination, ecological characteristics of mosquitoes such as hatching rate, transition rate, longevity, and oviposition rate had an inverted J shape with temperature, and the thresholds were 31.2 °C, 32.1 °C, 17.7 °C, and 25.7 °C, respectively. The parameter expressions of Aedes albopictus using meteorological factors as predictors under different stages were established. Meteorological factors especially temperature significantly influence the development of Aedes albopictus under different physiological stages. The established formulas of ecological parameters can provide important information for modeling mosquito-borne infectious diseases. [ABSTRACT FROM AUTHOR]

Background: Over the past decades, several viral diseases transmitted by Aedes mosquitoes—dengue, chikungunya, Zika—have spread outside of tropical areas. To limit the transmission of these viruses and preserve human health, the use of mosquito traps has been developed as a complement or alternative to other vector control techniques. The objective of this work was to perform a systematic review of the existing scientific literature to assess the efficacy of interventions based on adult mosquito trap to control Aedes population densities and the diseases they transmit worldwide. Methods and findings: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic review was conducted using the PubMed and Scopus databases. Among the 19 selected papers, lethal ovitraps were used in 16 studies, host-seeking female traps in 3 studies. Furthermore, 16 studies focused on the control of Ae. aegypti. Our review showed great heterogeneity in the indicators used to assess trap efficacy: e.g., the number of host-seeking females, the number of gravid females, the proportion of positive containers, the viral infection rate in female mosquitoes or serological studies in residents. Regardless of the type of studied traps, the results of various studies support the efficacy of mass trapping in combination with classical integrated vector control in reducing Aedes density. More studies with standardized methodology, and indicators are urgently needed to provide more accurate estimates of their efficacy. Conclusions: This review highlights gaps in the demonstration of the efficacy of mass trapping of mosquitoes in reducing viral transmission and disease. Thus, further large-scale cluster randomized controlled trials conducted in endemic areas and including epidemiological outcomes are needed to establish scientific evidence for the reduction of viral transmission risk by mass trapping targeting gravid and/or host-seeking female mosquitoes. Author summary: Over the past decades, several viral diseases transmitted by Aedes mosquitoes—dengue, chikungunya, Zika—have spread outside of tropical areas. To limit the transmission of these viruses and preserve environment and human health, the use of mosquito traps has been developed as a complement or alternative to other vector control techniques. This review support the efficacy of mass trapping in combination with classical integrated vector control on the reduction in mosquito vector populations within several weeks of deployment. However, this research highlights gaps in the demonstration of the efficacy of mass trapping of mosquitoes in reducing viral transmission and disease. Thus, further studies are needed to establish scientific evidence for the reduction of viral transmission risk by mass trapping targeting gravid and/or host-seeking female mosquitoes. [ABSTRACT FROM AUTHOR]

Background: In the last decades, Aedes albopictus has become an increasing public health threat in tropical as well as in more recently invaded temperate areas due to its capacity to transmit several human arboviruses, among which Dengue, Chikungunya and Zika. Enhancing the efficiency of currently used collection approaches, such as ovitraps and sticky traps, is desirable for optimal monitoring of the species abundance, for assessment of the risk of arbovirus transmission and for the optimisation of control activities. Findings: Two sets of 4 × 4 Latin-square experiments were carried out in Tirana (Albania) to test whether modifications in ovitrap shape and size and in oviposition substrate would increase collections of Ae. albopictus eggs and whether hay-infusion would increase adult catches by sticky trap. Generalized Linear Mixed Models with negative binomial error distribution were carried out to analyse the data. Cylindrical ovitraps lined with germination paper yielded significantly higher egg catches than those exploiting either the (commonly used) wooden paddles or floating polystyrene blocks as oviposition substrates. No difference was observed between cylindrical and conical shaped ovitraps. Ovitraps and sticky traps baited with hay infusion yielded significantly higher egg and adult catches than un-baited ones. A significant relationship between ovitrap and sticky trap catches was observed both in the absence and in the presence of attractants, with ovitrap catches increasing more than sticky trap catches at increasing adult female densities. Conclusions: This study provides grounds for optimisation of ovitraps and sticky traps as monitoring tools for Ae. albopictus by (i) supporting use of germination paper as most appropriate oviposition substrate; (ii) suggesting the possible use of stackable conical ovitraps for large scale monitoring; (iii) confirming the use of hay-infusion to increase egg catches in ovitraps, and showing that hay-infusion also significant increases adult catches by sticky traps. [ABSTRACT FROM AUTHOR]

This paper introduces a new mosquito images dataset that is suitable for training and evaluating a recognition system on mosquitoes in normal or smashed conditions. The images dataset served mainly for the development a machine learning model that can recognize the mosquito in the public community, which commonly found in the smashed/damaged form by human. Especially the images of mosquito in hashed condition, which to the best of our knowledge, a dataset that fulfilled such condition is not available. There are three mosquito species in the dataset, which are Aedes aegypti, Aedes albopictus and Culex quinquefasciatus, and the images were annotated until species level due to the specimen was purely bred in a WHO accredited breeding laboratory. The dataset consists of seven root files, six root files that composed of six classes (each species with either normal landing, or random damaged conditions) with a total of 1500 images, and one pre-processed file which consists of a train, test and prediction set, respectively for model construction. Measurement(s) recognition system with machine/deep learning Technology Type(s) Camera Device Sample Characteristic - Organism Aedes aegypti • Aedes albopictus [ABSTRACT FROM AUTHOR]

Pest management is looking for green and cost-effective innovative solutions to control tiger mosquitoes and other pests. By using biomimetic principles and biocompatible/biodegradable biopolymers, it could be possible to develop a new approach based on substrates that selectively attract insects by reproducing specific natural environmental conditions and then kill them by hosting and delivering a natural biopesticide or through mechanical action (biomimetic lure and kill approach, BL&K). Such an approach can be theoretically specialized against tiger mosquitoes (BL&K-TM) by designing hydrogels to imitate the natural oviposition site's conditions to employ them inside a lure and kill ovitraps as a biomimetic oviposition substrate. In this work, the hydrogels have been prepared to prove the concept. The study compares lab/on-field oviposition between standard substrates (absorbing paper/masonite) and a physical and chemically crosslinked hydrogel composition panel. Then the best performing is characterized to evaluate a correlation between the hydrogel's properties and oviposition. Tests identify a 2-Hydroxyethylcellulose (HEC)-based physical hydrogel preparation as five times more attractive than the control in a lab oviposition assay. When employed on the field in a low-cost cardboard trap, the same substrate is seven times more capturing than a standard masonite ovitrap, with a duration four times longer. [ABSTRACT FROM AUTHOR]

Understanding the relationship of population dynamics to density is central to many ecological investigations. Despite the importance of density-dependence in determining population growth, the empirical relationship between density and per capita growth remains understudied in most systems and is often assumed to be linear. In experimental studies of interspecific competition, investigators often evaluate the predicted outcomes by assuming such linear relationships, fitting linear functions, and estimating parameters of competition models. In this paper, we experimentally describe the shape of the relationship between estimated population rate of change and initial density using laboratory-reared populations of three mosquito species. We estimated per capita growth rate for these experimental populations over a 30-fold range of larval densities at a standard resource abundance. We then compared fits of linear models and several different nonlinear models for the relationship of estimated rate of change and density. We find that that the relationship between density and per capita growth is strongly non-linear in Aedes aegypti (Linnaeus), Aedes albopictus (Skuse), and Aedes triseriatus (Say) mosquitoes. Components of population growth (survivorship, development time, adult size) are also nonlinearly related to initial density. The causes and consequences of this nonlinearity are likely to be important issues for population and community ecology. [ABSTRACT FROM AUTHOR]

Vector-borne diseases are a major burden to the human health. Vector species of Aedes mosquitoes transmit several arboviral diseases, including dengue, chikungunya, yellow fever and Zika. According to the World Health Organization, the dengue virus causes about 390 million infections per year out of which almost 96 million have manifests clinically with high severity of the disease. For vector and pest control, excessive use of insecticides is the widest option (different sectors have used 451,000 metric tonnes of some selected technical grade pesticides that have been used from 2011–19 in various sectors). Data for the present review was collected from published papers from 1993 to 2019 from journal websites, google scholar, research gate. Dichlorodiphenyltrichloroethane (DDT) has been used since 1946 in malaria and pest control and results in widespread resistance in both vector species: Aedes aegypti and Aedes albopictus in almost all states of India. Aedes aegypti shows a high degree of resistance to permethrin, lambda-cyhalothrin, and cyfluthrin, while Aedes albopictus is still susceptible to these insecticides in most areas across the country. Enhanced activity of detoxifying enzymes such as cytochrome P450, glutathione-S-transferase, esterase, gamma-aminobutyric acid, and mixed-function oxidase was reported in resistant populations as compared to susceptible populations. Based on the review, we conclude that prolonged exposure to insecticides has led to the development of resistance against organochlorines, organophosphates, pyrethroids, and carbamates. Mixed and synchronized activities of these detoxifying enzymes are related to the development of resistance. As such, minimal and rotational use of insecticides can prevent the development of resistance in mosquitoes. [ABSTRACT FROM AUTHOR]

Aedesalbopictus mosquitoes overwinter as eggs in north-central Florida. Knowledge of this species' overwintering survival rate is of great interest to local mosquito control districts. In this study, field-collected Ae. albopictus eggs were exposed to natural conditions during winter 2016–17 in Gainesville, FL, to determine the overwintering survival rate. Individual strips of germination paper containing eggs of this species were collected from ovitraps in November 2016 then later clipped to the inner edge of empty flowerpots and placed in the Department of Public Works compound, City of Gainesville, in December. Egg strips that remained outdoors were later brought back to the laboratory and hatched in late March 2017. Significant differences were found among the 5 ambient environmental exposure methods (configurations) in terms of egg survival rate, egg collapsed rate, and egg unaccounted rate, whereas no significant difference was observed on the egg intact rate. Egg strips stored in flowerpots with the drainage hole sealed had the highest survival rate (18.3%), whereas eggs stored in an open covered area had the least survival rate (2.4%). The effect of different storage conditions on the survivorship of overwintering Ae. albopictus eggs in north-central Florida is discussed. [ABSTRACT FROM AUTHOR]

In this paper, we use the classical Ross–Macdonald model to analyze the effect of the Mosquito Home System (MHS), which is an example of an auto-dissemination trap, in controlling the spread of dengue in Malaysia in a high-rise condominium environment. By using the national dengue data from Malaysia, we are able to estimate ł which represents the initial growth rate of the dengue epidemic and thus allows us to estimate the number of mosquitoes in Malaysia. The basic reproduction number R 0 is also obtained. We have constructed a mathematical expression which allows us to estimate the potential number of breeding sites for Aedes mosquitoes. Later on, by using the data available from the 11 months trials carried out in three blocks of flats in Selangor, we improved on our dengue model by including the effect of the MHS and thus modeling the impact it has on the spread of dengue within the flats. Numerical simulations and tables are also produced to illustrate our results. [ABSTRACT FROM AUTHOR]

Dengue hemorrhagic fever is a worldwide epidemic caused by dengue virus and spread by infected female mosquitoes. The two main mosquito species vectors of the dengue virus are Aedes aegypti and Aedes albopictus. Conventionally, the identification of these two species' egg is time-consuming which makes vector control more difficult. However, although attempts on efficiency improvements by providing automatic identification have been conducted, the earliest stage is at the larval stage. In addition, there are currently no studies on classifying to distinguish the two vectors during the egg stage based on their digital image. A total of 140 egg images of Aedes aegypti and Aedes albopictus were collected and validated by rearing them individually to become adult mosquitoes. Image processing and elliptic Fourier analysis were carried out to extract and describe the shape difference of the two vectors' eggs. Machine learning algorithms were then used to classify the shape signatures. Morphometrically, the two species' eggs were significantly different, which Aedes albopictus were smaller in size. Egg-shape contour reconstructions of principal components and Multivariate Analysis of Variance (MANOVA) revealed that there is a significant difference (p value < 0.000 ) in shape between two species' eggs at the posterior end. Based on Wilk's lambda of the MANOVA results, the classification could be done using only the first 3 principal components. Classification of the test data yielded an accuracy of 85.00% and F1 score 84.21% with Linear Discriminant Analysis applying default hyperparameter. Alternatively, k-Nearest Neighbors with optimal hyperparameter yielded a higher classification result with 87.50% and 87.18% of accuracy and F1 score, respectively. These results demonstrate that the proposed method can be used to classify Aedes aegypti and Aedes albopictus eggs based on their digital image. This method provides a foundation for improving the identification and surveillance of the two vectors and decision making in developing vector control strategies. [ABSTRACT FROM AUTHOR]

Background and purpose: Considering the effects of dengue fever on the health of people in the society and the role of climatic and environmental factors on the spread of this disease, this review study has investigated the climatic and environmental factors affecting the prevalence of dengue fever. Materials and methods: In this review, the academic papers, in English and persian languages, published until the beginning of August 2022 were investigated. These articles were searched in scientific databasese of Scopus, ScienceDirect, PubMed, Web of Science and Google Scholar, using keywords such as "dengue fever", "dengue vector", "vector mosquito", "Aedes mosquito", "environmental factors", and "weather factors". Endnote X8 software was also used to organize, and study titles and abstracts of the articles. Results: Considering the impacts of environmental changes on mosquito population and its subsequent effect on the occurrence of dengue fever, the present study showed that changes in climatic factors including air temperature, rainfall, and humidity affect the frequency of dengue disease vectors. Land use and land cover change can affect mosquito population and dengue transmission by changing local ecology. Household waste and its accumulation around residential houses can also hold a significant amount of water as environmental pollutants and can be considered as a habitat for mosquito larvae such as Aedes species. Conclusion: The analysis of climatic and environmental factors has shown that environmental factors and weather changes can affect the occurrence of dengue fever. [ABSTRACT FROM AUTHOR]

Vector competence refers to the ability of a vector to acquire, maintain, and transmit a pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become the standard for approximating arbovirus transmission. However, this method is time-consuming and labor-intensive. Here we compare the capillary tube method to an alternative high-throughput detection method the collection of saliva on paper cards saturated with honey, with (FTA card) and without (filter paper) reagents for the preservation of nucleic acid for Aedes aegypti and Aedes albopictus mosquitoes infected with two emerging genotypes of the chikungunya virus (CHIKV). Model results showed that the Asian genotype CHIKV dissemination in the harvested legs of both Ae. aegypti and Ae. albopictus increased the odds of females having a positive salivary infection and higher salivary viral titers, while for the IOL genotype the same effect was observed only for Ae. aegypti. Of the three tested detection methods, the FTA card was significantly more effective at detecting infected saliva of Ae. aegypti and Ae. albopictus females than the capillary tube and filter paper was as effective as the capillary tube for the Asian genotype. We did not find significant effects of the detection method in detecting higher viral titer for both Asian and IOL genotypes. Our results are discussed in light of the limitations of the different tested detection methods. [ABSTRACT FROM AUTHOR]

Arthropod-borne infectious diseases cause many deaths and a major economic burden worldwide. Repellents play an important role in protecting people from infectious biting arthropods. The repellency of veratraldehyde, a known food additive, and the WJ-1041 formulation containing 10% veratraldehyde was tested against Aedes albopictus and Culex pipiens pallens females and Haemaphysalis longicornis nymphs using arm-in-cage, indoor or filter paper tests. Veratraldehyde exhibited repellency similar to or lower than that of n,n-diethyl-meta-toluamide (DEET) against A. albopictus, but in H. longicornis, the activity of veratraldehyde was better than that of DEET. The repellency of the 10% veratraldehyde solution was comparable to that of 20% DEET against the two mosquitoes. When comparing repellency between the WJ-1041 formulation (10% veratraldehyde) and 10% DEET against C. pipiens pallens, A. Albopictus and H. longicornis, the two showed similar repellency and complete protection time (CPT) values. However, there was a small difference depending on the tested insects. The absorption of veratraldehyde via skin was minimal, if at all. The pharmacokinetic parameters (Cmax and Tmax) of veratraldehyde in blood samples of rats were not different from those of the control group. Based on these results, veratraldehyde has high potential to be commercialized as a repellent agent against infectious disease-borne pests in the near future. [ABSTRACT FROM AUTHOR]

The present study was carried out to understand the impact of climatic conditions on adult life table parameters of Aedes albopictus (Skuse) (Diptera: Culicidae). Thus, strains of Aedes albopictus were collected from two different ecological regions of Rajasthan, India i.e., Kota (semi-arid) and Barmer (arid), and compared under insectary conditions. The result showed that male life expectancy was greater in the Kota strain (8.19 days) than in the Barmer strain (5.91 days) with significant differences whereas no significant differences were found for female life expectancy (Kota: 13.75 days; Barmer: 12.24 days). Barmer strain showed a higher intrinsic rate of increase (rm) (0.25), finite rate of increase (λ) (1.29), and lower doubling time (DT) (2.66 days) than the Kota strain (rm: 0.22; λ: 1.24; DT: 3.12 days) with significant differences which shows more r-strategic pattern in Barmer strain. However, the slobodkin curve for males and females of both strains showed a type II survivorship curve. No significant differences were found for net reproductive rate (R0), mean generation time (T), oviposition schedule, and fecundity parameters. Conclusively, the present study may help in the better prediction of Ae. albopictus population dynamics prevalent in these geographical areas and for implementing the efficient vector management practices. [ABSTRACT FROM AUTHOR]

Container-breeding mosquitoes are increasingly important in public health due to recent outbreaks of Zika virus, chikungunya, and dengue. This paper documents seasonality of the most prevalent container-breeding mosquito species in Mississippi—Aedes albopictus. Ten sites in 5 counties in both northern and central Mississippi (20 sites, 10 counties total) were sampled by larval dipping and oviposition traps biweekly from September 2016 to June 2019, totaling 22 months and potentially yielding 440 egg or larval collections. However, 22 collections were missed due to inclement weather and personnel issues during the study period, so actually only 418 site visits were performed. Sites were chosen to maximize chances of finding Ae. albopictus. Of the total 1,310 mosquito larvae collected during the study period, 717 larvae and 50 positive egg papers belonged to Ae. albopictus. Aedes albopictus was found in all 10 northern and central counties. No eggs were collected at any of the sites from December through February, although larvae were occasionally collected during that time frame. This study demonstrates that Ae. albopictus is active in central and northern Mississippi beginning in March each year and continuing through November or December. There is little activity during the coldest months of the year (January and February). These data represent the first extensive analysis of Ae. albopictus seasonality in Mississippi, and as such, allow for better public health awareness of diseases transmitted by this species and design of more effective vector control programs. [ABSTRACT FROM AUTHOR]

We examined the growth characteristics of Tolypocladium cylindrosporum IBT 41712 and its potential to infect eggs of Aedes aegypti and Ae. albopictus at a low temperature (15°C). When grown on Sabouraud dextrose agar supplemented with yeast extract, the IBT 41712 formed white colonies turning to a slightly darker, off-white color when mature. The mycelia bore swollen conidiophores producing smooth-walled, oblong to cylindrical conidia with varying sizes, ranging from 1.5 to 3.5 μm long. To determine the optimum temperature for the fungus, we cultured the fungus at eight temperatures (4°C, 12°C, 15°C, 21°C, 28°C, 33°C, 37°C, and 40°C) and measured the diametric growth. The optimum temperature for growth was 28°C since it had the highest diametric growth rate (2.1 ± 0.05 mm/day) and the fastest sporulation period (within 8-10 days of incubation). There was no fungal growth at the 3 highest temperatures (33°C, 37°C, and 40°C) but plates incubated at 33°C, when shifted to optimal temperature (28°C), showed visible growth indicating that following incubation at 33°C, the fungus remained viable. The IBT 41712 successfully infected mosquito eggs at 15°C. Fungal treatment induced egg hatch on moist seed-germination paper and this effect was more pronounced in Ae. aegypti compared to Ae. albopictus. When treated eggs were immersed in dH2O 21 days posttreatment, larval hatch of both Ae. aegypti (control = 91%, 1 × 107 conidia/ml, fungal treatment = 0%) and Ae. albopictus (control = 85%, fungal treatment = 28%) was significantly lower in fungal treatment compared to the controls. The ability of the strain to grow in a wide temperature range, and effectively infect mosquito eggs and induce egg hatch at a low temperature warrants further investigation for its potential as a mosquito control agent targeting eggs that overwinter or undergo long diapause. [ABSTRACT FROM AUTHOR]

Chikungunya virus (CHIKV) is a mosquito-borne infection that is emerging in temperate areas of Europe, following the expansion of one of its vector species, Aedes albopictus. Although CHIKV fever is a self-limiting disease, with a clinical syndrome often resolving within few days, it can also cause severe sequelae, including chronic polyarthralgia lasting up to 5 years. Additionally, CHIKV outbreaks may limit blood bank donations, adding economic burden on the health system. Public health authorities in Europe need to increase their preparedness against this emerging threat. Two large CHIKV outbreaks occurred in Italy in 2007 and 2017, with hundreds of cases and significant geographical spread. The aim of this paper is to review and compare the 2 Italian outbreaks in terms of available estimates of key epidemiological features, patient clinical presentation, virus and immunological characteristics, and public health response. Recommendations for public health and future directions for research are also discussed and highlighted. Key results: Both outbreaks started in small towns, but cases were also detected in nearby larger cities where transmission was limited to small clusters. The time spans between the first and the last symptom onsets were similar between the 2 outbreaks, and the delay from the symptom onset of the index case and the first case notified was considerable. Comparable infection and transmission rates were observed in laboratory. The basic reproductive number (R0) was estimated in the range of 1.8–6 (2007) and 1.5–2.6 (2017). Clinical characteristics were similar between outbreaks, and no acute complications were reported, though a higher frequency of ocular symptoms, myalgia, and rash was observed in 2017. Very little is known about the immune mediator profile of CHIKV-infected patients during the 2 outbreaks. Regarding public health responses, after the 2007 outbreak, the Italian Ministry of Health developed national guidelines to implement surveillance and good practices to prevent and control autochthonous transmission. However, only a few regional authorities implemented it, and the perception of outbreak risk and knowledge of clinical symptoms and transmission dynamics by general practitioners remained low. Major conclusions: Efforts should be devoted to developing suitable procedures for early detection of virus circulation in the population, possibly through the analysis of medical records in near real time. Increasing the awareness of CHIKV of general practitioners and public health officials through tailored education may be effective, especially in small coastal towns where the outbreak risk may be higher. A key element is also the shift of citizen awareness from considering Aedes mosquitoes not only as a nuisance problem but also as a public health one. We advocate the need of strengthening the surveillance and of promoting the active participation of the communities to prevent and contain future outbreaks. Author summary: In Europe, vector-borne diseases have been increasing during the last decades. CHIKV is an example of a neglected emerging disease transmitted by the alien mosquitoes Ae. albopictus that caused 2 large outbreaks in Italy in 2007 and 2017. It is important to compare the main epidemiological, clinical, virological, and immunological features, as well as the public health responses, to increase preparedness to face future outbreaks. [ABSTRACT FROM AUTHOR]

Recently a major outbreak of chikungunya affected several countries of the world. In India only 62,628 and 64,057 clinically suspect chikungunya cases in 2017 and 2016 respectively and about 9,175 suspected chikungunya cases through April 2018. A self-remitting febrile viral illness, chikungunya fever caused/spread by Aedes aegypti and Aedes albopictus. The name has been originating from a verb in the Kimakonde language, meaning 'to become contorted' means 'stooped' appearance of joint, signalized by fever and joint pain which can sustain for months or even years in some of the patients. Although many medications are available in the market but the effective treatment against the viral infection is still restricted, due to drug resistance, side effects and toxicity. Focusing the problem, this paper mainly concerns on the cause, transmission of chik virus, and how herbal plants, their active constituents and specific medicinal properties used for relieving symptoms and for cure-providing an alternative way for treating chikungunya. [ABSTRACT FROM AUTHOR]

Background: The international movement of used tyres is a major factor responsible for global introductions of Aedes invasive mosquitoes (AIMs) (Diptera: Culicidae) that are major disease vectors (e.g. dengue, Zika, chikungunya and yellow fever). Surveillance methods are restricted by expense, availability and efficiency to detect all life stages. Currently, no tested method exists to screen imported used tyres for eggs in diapause, the life stage most at risk from accidental introduction. Here we test the efficiency of adhesive tape as an affordable and readily available material to screen tyres for eggs, testing its effect on hatch rate, larval development, DNA amplification and structural damage on the egg surface. Results: We demonstrated that the properties of adhesive tape can influence pick up of dormant eggs attached to dry surfaces. Tapes with high levels of adhesion, such as duct tape, removed eggs with high levels of efficiency (97% ± 3.14). Egg numbers collected from cleaned used tyres were found to explain larval hatch rate success well, particularly in subsequent larval to adult emergence experiments. The strength of this relationship decreased when we tested dirty tyres. Damage to the exochorion was observed following scanning electron microscopy (SEM), possibly resulting in the high variance in the observed model. We found that five days was the optimal time for eggs to remain on all tested tapes for maximum return on hatch rate success. Tape type did not inhibit amplification of DNA of eggs from three, five or ten days of exposure. Using this DNA, genotyping of AIMs was possible using species-specific markers. Conclusions: We demonstrated for the first time that adhesive tapes are effective at removing AIM eggs from tyres. We propose that this method could be a standardised tool for surveillance to provide public health authorities and researchers with an additional method to screen tyre cargo. We provide a screening protocol for this purpose. This method has a global applicability and in turn can lead to increased predictability of introductions and improve screening methods at high risk entry points. [ABSTRACT FROM AUTHOR]

Mosquito‐borne diseases resulting from the expansion of two key vectors, Aedes aegypti and Aedes albopictus (Diptera: Culicidae), continue to challenge whole regions and continents around the globe. In recent years there have been human cases of disease associated with Chikungunya, dengue and Zika viruses. In Europe, the expansion of Ae. albopictus has resulted in local transmission of Chikungunya and dengue viruses. This paper considers the risk that Ae. aegypti and Ae. albopictus represent for the U.K. and details the results of mosquito surveillance activities. Surveillance was conducted at 34 points of entry, 12 sites serving vehicular traffic and two sites of used tyre importers. The most common native mosquito recorded was Culex pipiens s.l. (Diptera: Culicidae). The invasive mosquito Ae. albopictus was detected on three occasions in southern England (September 2016, July 2017 and July 2018) and subsequent control strategies were conducted. These latest surveillance results demonstrate ongoing incursions of Ae. albopictus into the U.K. via ground vehicular traffic, which can be expected to continue and increase as populations in nearby countries expand, particularly in France, which is the main source of ex‐continental traffic. [ABSTRACT FROM AUTHOR]

• A dengue model is proposed with parameters linked to climate and human factors. • Both local and global stability of the disease-free equilibrium are analyzed. • Sensitive analysis reveals a decisive role of temperature in dengue spread. • Sensitive analysis discloses a local role of precipitation and human population. • Climate changes aggregate dengue burden more than human population. Dengue fever (DF) is a re-emerging disease spread by mosquitoes. It is primarily found in the tropics and subtropics, and its prevalence is heavily influenced by global warming. A comprehensive understanding of how the changes in climate and human population affect future dengue epidemics is crucial for effective prevention and control. In this paper we extend a mechanistic compartmental model for mosquito dynamics of immature and mature stages, coupled with human population dynamics. Stability analysis of the disease-free equilibrium is carried out analytically, and the basic reproduction number is derived. Model parameters are explicitly linked to climatic variables and human population density. Model validation suggests that the basic reproduction number acts as a reliable measure of the spread ability of DF in response to future scenarios of climate and human population change. Our model predicts that the risk area of DF will expand considerably in mainland China, and the expansion fronts are mainly extended in the center and east. Most importantly, sensitivity analysis and model prediction both show that while temperature is critical in determining the threshold suitability for DF transmission, precipitation and human population density act locally to regulate the spreading DF patterns, where high values of these two factors may significantly accelerate the spreading process. Our results indicate a strong call for increased attention in the areas that are currently safe from DF, but climatically suitable for DF spread as consequence of the fast-economic growth and rapid urbanization. [ABSTRACT FROM AUTHOR]

Aedes (Stegomyia) albopictus (Skuse, 1854) was reported in Brazil for the first time in 1986 and has shown marked expansion throughout the Brazilian territory. During a routine activity to control dengue fever conducted by the Division of Entomology of the Municipal Health Department in Rio Branco city, adults and immatures of Culicidae were collected in a peri-urban area. The identified Culicidae forms indicated that they belonged to the species Ae. albopictus. This is the first official record of the presence of Ae. albopictus in the Acre State, confirming its current presence in all Brazilian states. [ABSTRACT FROM AUTHOR]