1. Insect-specific viruses regulate vector competence in Aedes aegypti mosquitoes via expression of histone H4
- Author
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Merril Wongsokarijo, Betania Paiva Drumond, Koenraadt Cjm, Todjro Ymh, Flavia Viana Ferreira, Vilela App, Parra Mcp, Juliana N. Armache, Alioune Gaye, João Trindade Marques, de Faria Ijs, Avelino-Silva, Jean-Luc Imler, Prieto Mt, Tessa M. Visser, Obame-Nkoghe J, Silva Ats, de Almeida Jpp, Cruz Alc, Maurício Lacerda Nogueira, Roenick P. Olmo, Tan Ch, Mamadou Alpha Diallo, Eric Marois, Aguiar Ergr, Borges Maz, Christophe Paupy, Mota Rn, Luigi Sedda, de Souza Kpr, and Erna Geessien Kroon
- Subjects
Aedes ,Aedes albopictus ,biology ,viruses ,fungi ,virus diseases ,Aedes aegypti ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,medicine.disease ,Arbovirus ,Virology ,Virus ,Dengue fever ,Vector (epidemiology) ,medicine ,Human virome - Abstract
Aedes aegypti and Aedes albopictus are major mosquito vectors for arthropod-borne viruses (arboviruses) such as dengue (DENV) and Zika (ZIKV) viruses. Mosquitoes also carry insect-specific viruses (ISVs) that may affect the transmission of arboviruses. Here, we analyzed the global virome in urban Aedes mosquitoes and observed that two insect-specific viruses, Phasi Charoen-like virus (PCLV) and Humaita Tubiacanga virus (HTV), were the most prevalent in A. aegypti worldwide except for African cities, where transmission of arboviruses is low. Spatiotemporal analysis revealed that presence of HTV and PCLV led to a 200% increase in the chances of having DENV in wild mosquitoes. In the laboratory, we showed that HTV and PCLV prevented downregulation of histone H4, a previously unrecognized proviral host factor, and rendered mosquitoes more susceptible to DENV and ZIKV. Altogether, our data reveals a molecular basis for the regulation of A. aegypti vector competence by highly prevalent ISVs that may impact how we analyze the risk of arbovirus outbreaks.
- Published
- 2021
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