10 results on '"Secundino, Nágila Francinete Costa"'
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2. The larval midgut of Anopheles, Aedes, and Toxorhynchites mosquitoes (Diptera, Culicidae): a comparative approach in morphophysiology and evolution.
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Godoy, Raquel Soares Maia, Barbosa, Renata Cristina, Huang, Wei, Secundino, Nágila Francinete Costa, Pimenta, Paulo Filemon Paolucci, Jacobs-Lorena, Marcelo, and Martins, Gustavo Ferreira
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ANOPHELES ,AEDES ,COMPARATIVE method ,DIPTERA ,ANOPHELES gambiae ,AEDES aegypti ,MOSQUITOES ,LARVAE - Abstract
The mosquito larval midgut is responsible for acquiring and storing most of the nutrients that will sustain the events of metamorphosis and the insect's adult life. Despite its importance, the basic biology of this larval organ is poorly understood. To help fill this gap, we carried out a comparative morphophysiological investigation of three larval midgut regions (gastric caeca, anterior midgut, and posterior midgut) of phylogenetically distant mosquitoes: Anopheles gambiae (Anopheles albimanus was occasionally used as an alternate), Aedes aegypti, and Toxorhynchites theobaldi. Larvae of Toxorhynchites mosquitoes are predacious, in contrast to the other two species, that are detritivorous. In this work, we show that the larval gut of the three species shares basic histological characteristics, but differ in other aspects. The lipid and carbohydrate metabolism of the An. gambiae larval midgut is different compared with that of Ae. aegypti and Tx. theobaldi. The gastric caecum is the most variable region, with differences probably related to the chemical composition of the diet. The peritrophic matrix is morphologically similar in the three species, and processes involved in the post-embryonic development of the organ, such as cell differentiation and proliferation, were also similar. FMRF-positive enteroendocrine cells are grouped in the posterior midgut of Tx. theobaldi, but individualized in An. gambiae and Ae. aegypti. We hypothesize that Tx. theobaldi larval predation is an ancestral condition in mosquito evolution. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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3. Vector Competence for Zika Virus Changes Depending on the Aedes aegypti 's Region of Origin in Manaus: A Study of an Endemic Brazilian Amazonian City.
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da Costa Paz, Andréia, Chaves, Bárbara Aparecida, Godoy, Raquel Soares Maia, Coelho, Deilane Ferreira, Vieira Júnior, Ademir Bentes, Alencar, Rodrigo Maciel, Alcântara, João Arthur, Félix, Luiza dos Santos, Oliveira, Cinthia Catharina Azevedo, Monteiro, Wuelton Marcelo, Lacerda, Marcus Vinicius Guimarães, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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ZIKA virus ,AEDES aegypti ,ZIKA virus infections ,VIRAL transmission ,MOSQUITO control ,SALIVARY glands - Abstract
Zika virus (ZIKV) is transmitted to humans by the infectious bite of mosquitoes such as Aedes aegypti. In a city, the population control of mosquitoes is carried out according to alerts generated by different districts via the analysis of the mosquito index. However, we do not know whether, besides mosquito abundance, the susceptibility of mosquitoes could also diverge among districts and thus impact the dissemination and transmission of arboviruses. After a viremic blood meal, the virus must infect the midgut, disseminate to tissues, and reach the salivary gland to be transmitted to a vertebrate host. This study evaluated the patterns of ZIKV infection in the Ae. aegypti field populations of a city. The disseminated infection rate, viral transmission rate, and transmission efficiency were measured using quantitative PCR at 14 days post-infection. The results showed that all Ae. aegypti populations had individuals susceptible to ZIKV infection and able to transmit the virus. The infection parameters showed that the geographical area of origin of the Ae. aegypti influences its vector competence for ZIKV transmission. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Dengue and Zika virus infection patterns vary among Aedes aegypti field populations from Belo Horizonte, a Brazilian endemic city.
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Godoy, Raquel Soares Maia, Felix, Luiza dos Santos, Orfanó, Alessandra da Silva, Chaves, Bárbara Aparecida, Nogueira, Paula Monalisa, Costa, Breno dos Anjos, Soares, Aline Silva, Oliveira, Cinthia Catharina Azevedo, Nacif-Pimenta, Rafael, Silva, Breno Mello, Duarte, Ana Paula, Lacerda, Marcus Vinicius Guimarães de, Monteiro, Wuelton Marcelo, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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DENGUE hemorrhagic fever ,AEDES aegypti ,ZIKA virus infections ,DENGUE viruses ,GENETIC vectors ,VIRUS diseases - Abstract
Dengue virus (DENV) and Zika virus (ZIKV) belong to the same viral family, the Flaviviridae. They cause recurring threats to the public health systems of tropical countries such as Brazil. The primary Brazilian vector of both viruses is the mosquito Aedes aegypti. After the mosquito ingests a blood meal from an infected person, the viruses infect and replicate in the midgut, disseminate to secondary tissues and reach the salivary gland (SG), where they are ready to be transmitted to a vertebrate host. It is thought that the intrinsic discrepancies among mosquitoes could affect their ability to deal with viral infections. This study confirms that the DENV and ZIKV infection patterns of nine Ae. aegypti field populations found in geographically separate health districts of an endemic Brazilian city vary. We analyzed the infection rate, disseminated infection, vector competence, and viral load through quantitative PCR. Mosquitoes were challenged using the membrane-feeding assay technique and were tested seven and fourteen days post-infection (early and late infection phases, respectively). The infection responses varied among the Ae. aegypti populations for both flaviviruses in the two infection phases. There was no similarity between DENV and ZIKV vector competencies or viral loads. According to the results of our study, the risk of viral transmission overtime after infection either increases or remains unaltered in ZIKV infected vectors. However, the risk may increase, decrease, or remain unaltered in DENV-infected vectors depending on the mosquito population. For both flaviviruses, the viral load persisted in the body even until the late infection phase. In contrast to DENV, the ZIKV accumulated in the SG over time in all the mosquito populations. These findings are novel and may help direct the development of control strategies to fight dengue and Zika outbreaks in endemic regions, and provide a warning about the importance of understanding mosquito responses to arboviral infections. Author summary: Dengue and Zika are neglected diseases caused by viruses transmitted to humans by mosquitoes (vector-borne diseases). The primary vector of both diseases is Aedes aegypti, a highly abundant mosquito in tropical countries and adapted to the urban habitat. The viral cycle in the vector starts when the mosquito bites an infected person and acquires the viruses through the blood meal. When the infected blood reaches the mosquito's midgut, the viruses invade the epithelial cells and disseminate in several organs until they reach the salivary glands, enabling viral transmission to the next person. However, the mosquitoes have developed strategies to combat the viral invasion and dissemination in their body, making this journey a challenge to the viruses. Herein, we show that the mosquito responses against dengue and Zika viruses are distinct. In addition, mosquitoes from separate populations of the same city have different abilities to deal with the viruses in both cases, dengue and Zika infections. Our results show the diversity of responses that the mosquitoes may present to viral infections. These findings may better direct disease control strategies to combat dengue and Zika outbreaks in endemic regions. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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5. Prevención domiciliar de la dengue: evaluación preliminar de tela protectora para platos de macetas de planta
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Schall, Virgínia Torres, Barros, Héliton da Silva, Jardim, João Bosco, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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Factores de Riesgo ,Vigilancia Epidemiológica ,Ovipostura ,Evidengue® ,Dengue, epidemiología ,Aedes aegypti ,Capa de tela ,Oviposition ,Estudios Ecológicos ,Prato de vasos de planta ,Factores Socioeconómicos ,Mesh covers ,Flowerpot saucers - Abstract
Avaliou-se em laboratório a eficácia de um protótipo de capa de tela de poliéster (evidengue®) destinada a vedar o acesso de fêmeas do mosquito Aedes aegypti a pratos de vasos de planta. Dois pratos de vasos com água foram envolvidos individualmente com a capa e colocados com os seus respectivos vasos em duas gaiolas entomológicas, um em cada gaiola. Numa terceira gaiola foi colocado um conjunto idêntico de prato e vasos sem a capa. Cada gaiola recebeu 20 fêmeas copuladas do mosquito, alimentadas com sangue de camundongo. Os resultados mostram que a capa foi eficaz como barreira ao acesso de fêmeas. Novos testes são necessários para se avaliar a eficácia da capa como dispositivo de prevenção da ovipostura nos pratos. The effectiveness of a polyester mesh cover (evidengue®), aimed at preventing the access of female Aedes aegypti mosquitoes to flowerpot saucers, was evaluated in laboratory. Two saucers of flowerpot with water were individually wrapped with the cover was placed with their respective pots in two entomological cages. One identical set of flowerpot and saucer was placed in a third cage. In each cage, 20 gravid females, fed on mouse blood, were released. Results show that the cover was effective to prevent access of females. Further tests are necessary to assess cover effectiveness as a device to prevent saucer oviposition. Se evaluó en laboratorio la eficiencia de una cubierta de tela de polyester (evidengue®) con el objetivo de vedar el acceso de hembras del mosquito Aedes aegypti a platos de macetas de planta. Fueron utilizadas tres jaulas entomológicas, dos de ellas con evidengue® y una para control. En cada jaula fueron colocados dos conjuntos de macetas de planta y platos y 20 hembras copuladas, alimentadas con sangre de ratón. Los resultados muestran que la cubierta fue eficaz como barrera al acceso de hembras. Nuevas pruebas son necesarias para evaluar la eficiencia de la cubierta como dispositivo de prevención de la oviposición en los platos.
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- 2009
6. Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring.
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da Costa, Cristiano Fernandes, dos Passos, Ricardo Augusto, Lima, José Bento Pereira, Roque, Rosemary Aparecida, de Souza Sampaio, Vanderson, Campolina, Thais Bonifácio, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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DENGUE ,AEDES aegypti ,DENGUE viruses ,POLYMERASE chain reaction ,SEROTYPES ,INFECTIOUS disease transmission - Abstract
Background: Transovarial transmission of dengue virus in Aedes spp. mosquitoes is considered an important mechanism for the maintenance of the virus in nature and may be implicated in the occurrence of outbreaks and epidemics of the disease. However, there are few studies involving transovarial transmission and viral vector monitoring as a surveillance tool and control strategy. The present study evaluated transovarial transmission of dengue virus in Aedes aegypti populations as a xenomonitoring strategy in municipalities of the Amazonas state. Results: Aedes sp. eggs (13.164) were collected, with 30% viability of third- and fourth-instar larvae. Transovarial transmission of DENV was detected in all municipalities. The transovarial infection rate (TOR) in the municipalities was 46% of the DENV positive samples. The minimum infection rate (MIR) was 17.7 in the state, varying from 11.4 to 24.1 per 1,000 larvae tested in the respective municipalities. Four DENV serotypes were identified, with DENV I and IV being present in all municipalities investigated. The number of reported dengue fever cases varied during this period. Conclusions: Our results suggest that transovarial transmission may be an important mechanism for the maintenance and spreading of the disease in Amazonas municipalities. Using qRT-PCR, it was possible to identify the four DENV serotypes in larval samples. The methodology used in the present study proved suitable as a DENV xenomonitoring model in immature mosquitoes, contributing to the development of systems for early detection of viral circulation and predictive models for the occurrence of outbreaks and epidemics of this disease. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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7. Dengue Infection Susceptibility of Five Aedes aegypti Populations from Manaus (Brazil) after Challenge with Virus Serotypes 1–4.
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Chaves, Bárbara Aparecida, Godoy, Raquel Soares Maia, Campolina, Thaís Bonifácio, Júnior, Ademir Bentes Vieira, Paz, Andréia da Costa, Vaz, Evelyn Beatriz da Costa, Silva, Breno Mello, Nascimento, Rêgila Mello, Guerra, Maria das Graças Vale Barbosa, Lacerda, Marcus Vinicius Guimarães, Monteiro, Wuelton Marcelo, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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AEDES aegypti ,DENGUE viruses ,DENGUE ,SEROTYPES ,VIRAL load ,VIRAL variation ,VIRAL transmission ,ARBOVIRUS diseases - Abstract
The successful spread and maintenance of the dengue virus (DENV) in mosquito vectors depends on their viral infection susceptibility, and parameters related to vector competence are the most valuable for measuring the risk of viral transmission by mosquitoes. These parameters may vary according to the viral serotype in circulation and in accordance with the geographic origin of the mosquito population that is being assessed. In this study, we investigated the effect of DENV serotypes (1–4) with regards to the infection susceptibility of five Brazilian Ae. aegypti populations from Manaus, the capital of the state of Amazonas, Brazil. Mosquitoes were challenged by oral infection with the DENV serotypes and then tested for the presence of the arbovirus using quantitative PCR at 14 days post-infection, which is the time point that corresponds to the extrinsic incubation period of Ae. aegypti when reared at 28 °C. Thus, we were able to determine the infection patterns for DENV-1, -2, -3 and -4 in the mosquito populations. The mosquitoes had both interpopulation and inter-serotype variation in their viral susceptibilities. All DENV serotypes showed a similar tendency to accumulate in the body in a greater amount than in the head/salivary gland (head/SG), which does not occur with other flaviviruses. For DENV-1, DENV-3, and DENV-4, the body viral load varied among populations, but the head/SG viral loads were similar. Differently for DENV-2, both body and head/SG viral loads varied among populations. As the lack of phenotypic homogeneity represents one of the most important reasons for the long-term fight against dengue incidence, we expect that this study will help us to understand the dynamics of the infection patterns that are triggered by the distinct serotypes of DENV in mosquitoes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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8. Prevenção domiciliar da dengue: avaliação preliminar de tela protetora para pratos de vasos de planta.
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Schall, Virgínia Torres, Barros, Héliton da Silva, Jardim, João Bosco, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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- 2009
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9. Role of Egg Buster in Hatching of Aedes aegypti: Scanning Electron Microscopy Study
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Pereira, Sheila Tavares, Secundino, Nágila Francinete Costa, Carvalho Botelho, Ana Cristina, Pinheiro, Valéria Cristina, Tadei, Wanderly Pedro, and Pimenta, Paulo Filemon Paolucci
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- 2006
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10. Simultaneous circulation of zakat, dengue, and chikungunya viruses and their vertical co-transmission among Aedes aegypti.
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Teixeira, Adriano Fernandes, de Brito, Breno Bittencourt, Correia, Thiago Macêdo Lopes, Viana, Airton Idalécio Sousa, Carvalho, Juliana Cabral, da Silva, Filipe Antônio França, Santos, Maria Luísa Cordeiro, da Silveira, Eliezer Almeida, Neto, Henry Paul Granger, da Silva, Natalia Maria Pereira, Rocha, Cínthya Viana Souza, Pinheiro, Fillipe Dantas, Chaves, Bárbara Aparecida, Monteiro, Wuelton Marcelo, de Lacerda, Marcus Vinícius Guimarães, Secundino, Nágila Francinete Costa, Pimenta, Paulo Filemon Paolucci, and de Melo, Fabrício Freire
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AEDES aegypti , *CHIKUNGUNYA virus , *ARBOVIRUS diseases , *ZAKAT , *DENGUE , *DENGUE viruses , *COMORBIDITY - Abstract
• Aedes aegypti larvae can be simultaneously infected by two arboviruses. • A vertical cotransmission of arboviruses to Ae. aegypti larvae may occur. • Ae. aegypti infestation indexes seem to be able to predict arboviral circulation. The outbreaks caused by the Aedes aegypti -transmitted dengue virus (DENV), zakat virus (ZIKV), and chikungunya virus (CHIKV) result in a significant impact to the health systems of tropical countries. Furthermore, the occurrence of patients coinfected by at least two of these arboviruses is an aggravating factor in that scenario. On this basis, surveillance tools such as the Rapid Index Survey for Aedes aegypti (LIRAa) are used to estimate vector infestation in order to improve the prediction of human outbreaks. Ae. aegypti eggs were collected in the city of Vitória da Conquista, in Bahia State, Brazil, and subsequently hatched into larvae, which were analyzed in pools or individually for the presence of DENV, ZIKV, and CHIKV by molecular biology methods. The detection data for arboviruses were crossed with the LIRAa obtained in each region of the study city. Thirty larvae pools were analyzed, and fourteen (46.6%) of them were detected positive for DENV, ZIKV, and/or CHIKV. Among the individually analyzed larvae (n = 30), nine (30%) were positive for any of these arboviruses, and four (13.3%) were simultaneously coinfected by DENV and ZIKV. Furthermore, there was a positive correlation between the detection of circulating arboviruses and LIRAa. The simultaneous Ae. aegypti larvae infection by two different arboviruses is an unprecedented finding. This result suggests the occurrence of a vertical arboviruses co-transmission from the female mosquito to its offspring in nature. The occurrence of concomitant circulation of DENV, ZIKV, and CHIKV in Ae. aegypti from a single study region is another finding of this article. Finally, LIRAa seems to not only estimate vector infestation but also to predict circulation of arboviruses. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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