1. Chemical composition and biological activities of the essential oils from Vitex-agnus castus, Ocimum campechianum and Ocimum carnosum.
- Author
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Ricarte LP, Bezerra GP, Romero NR, Silva HCD, Lemos TLG, Arriaga AMC, Alves PB, Santos MBD, Militão GCG, Silva TDS, Braz-Filho R, and Santiago GMP
- Subjects
- Animals, Biological Assay, Cell Line, Tumor drug effects, Gas Chromatography-Mass Spectrometry, Humans, Insecticides isolation & purification, Oils, Volatile chemistry, Oils, Volatile isolation & purification, Plant Leaves chemistry, Plant Oils chemistry, Toxicity Tests, Vitex classification, Aedes drug effects, Insecticides pharmacology, Larva drug effects, Ocimum chemistry, Oils, Volatile pharmacology, Plant Oils pharmacokinetics, Vitex chemistry
- Abstract
The essential oils obtained by hydrodistillation from fresh leaves of Vitex agnus-castus and Ocimum campechianum, and from fresh inflorescences of Ocimum carnosum were analysed by GC-FID and GC-MS. The major components of V. agnus-castus essential oil were identified as 1,8-cineole (47.9%), terpinyl α-acetate (11.6%), sabinene (11.2%) and caryophyllene oxide (9.7%), while in the O. campechianum essential oil were eugenol (72.1%), β-elemene (6.8%), (E)-caryophyllene (6.4%) and bicyclogermacrene (5.2%). Linalool (79.0%), α-epi-cadinol (5.4%), terpinen-4-ol (3.2%) and 1,8-cineole (2.8%) were the major constituents in the O. carnosum essential oil. The essential oils were subsequently evaluated for their larvicidal and cytotoxic activities. Larval bioassay against Aedes aegypti of V. agnus-castus, O. campechianum and O. carnosum essential oils showed LC50 values of 97.55 ± 0.35, 81.45 ± 0.35 and 109.49 ± 0.35 μg/mL, respectively. The in vitro cytotoxic activities of the essential oils has been evaluated on breast adenocarcinoma (MCF-7), lung carcinoma (NCI-H292), pro-myelocytic leukemia (HL-60), and cervical adenocarcinoma (HEP-2) human cell lines, and pro-myelocytic leukemia cells lines (HL-60) were found to be the most sensitive to all the essential oils tested than the others. This is the first report on larvicidal and cytotoxic activities of these essential oils.
- Published
- 2020
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