1. Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae.
- Author
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Pessanha JE, Caiaffa WT, Cecilio AB, Iani FC, Araujo SC, Nascimento JC, Kroon EG, Proietti FA, and Arias JR
- Subjects
- Aedes classification, Animals, Dengue transmission, Dengue virology, Dengue Virus classification, Dengue Virus genetics, Insect Vectors classification, Larva virology, RNA, Viral analysis, Reverse Transcriptase Polymerase Chain Reaction, Aedes virology, Dengue Virus isolation & purification, Insect Vectors virology
- Abstract
Introduction: To detect dengue virus, eggs of Aedes sp were collected in the city of Belo Horizonte, Brazil, in 2007., Methods: Egg samples were subsequently hatched and the larvae were tested for the presence of dengue virus RNA by RT-PCR., Results: Among the Aedes aegypti larvae samples, 163 (37.4%) out of 435 were positive, including 32 (10.9%) of 293 individual larvae samples concomitantly positive for two serotypes., Conclusions: Virological surveillance detecting coinfected vectors in the field could represent an important strategy for understanding the numerous factors involved in the transmission and clinical presentation of dengue.
- Published
- 2011
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