27 results on '"Mori, Naoki"'
Search Results
2. A New Strategy for Adult T‐Cell Leukemia Treatment Targeting Glycogen Synthase Kinase‐3β.
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Ishikawa, Chie and Mori, Naoki
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GLYCOGEN synthase kinase , *REACTIVE oxygen species , *CELL cycle , *FLOW cytometry , *CELL proliferation , *LACTATES - Abstract
ABSTRACT Objectives Methods Results Conclusion The role of glycogen synthase kinase (GSK)‐3β in adult T‐cell leukemia (ATL) caused by human T‐cell leukemia virus type 1 (HTLV‐1) is paradoxical and enigmatic. Here, we investigated the role of GSK‐3β and its potential as a therapeutic target for ATL.Cell proliferation/survival, cell cycle, apoptosis, and reactive oxygen species (ROS) generation were examined using the WST‐8 assay, flow cytometry, and Hoechst 33342 staining, respectively. Expression of GSK‐3β and cell cycle/death‐related proteins, and survival signals was analyzed using RT‐PCR, immunofluorescence staining, and immunoblotting.HTLV‐1‐infected T‐cell lines showed nuclear accumulation of GSK‐3β. GSK‐3β knockdown and its inhibition with 9‐ING‐41 and LY2090314 suppressed cell proliferation/survival. 9‐ING‐41 induced G2/M arrest by enhancing the expression of γH2AX, p53, p21, and p27, and suppressing the expression of CDK1, cyclin A/B, and c‐Myc. It induced caspase‐mediated apoptosis by decreasing the expression of Bcl‐xL, Mcl‐1, XIAP, c‐IAP1/2, and survivin, and increasing the expression of Bak and Bax. 9‐ING‐41 also induced ferroptosis and necroptosis, promoted JNK phosphorylation, and suppressed IKKγ and JunB expression. It inhibited the phosphorylation of IκBα, Akt, and STAT3/5, induced ROS production, and reduced glycolysis‐derived lactate levels.GSK‐3β functions as an oncogene in ATL and could be a potential therapeutic target. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Pivotal role of dihydroorotate dehydrogenase as a therapeutic target in adult T‐cell leukemia.
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Ishikawa, Chie and Mori, Naoki
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ADULT T-cell leukemia , *DIHYDROOROTATE dehydrogenase , *HTLV-I - Abstract
Objectives: We aimed to determine the role of dihydroorotate dehydrogenase (DHODH) in pathogenesis of adult T‐cell leukemia (ATL) caused by human T‐cell leukemia virus type 1 (HTLV‐1) and the effects of its inhibition on the de novo pyrimidine biosynthesis pathway. Methods: Cell proliferation, viability, cycle, and apoptosis were analyzed using WST‐8 assays, flow cytometry, and Hoechst 33342 staining. To elucidate the molecular mechanisms involved in the anti‐ATL effects of DHODH knockdown and inhibition, RT‐PCR and immunoblotting were conducted. Results: HTLV‐1‐infected T‐cell lines aberrantly expressed DHODH. Viral infection and the oncoprotein, Tax, enhanced DHODH expression, while knockdown of DHODH decreased HTLV‐1‐infected T‐cell growth. In addition, BAY2402234, a DHODH inhibitor, exerted an anti‐proliferative effect, which was reversed by uridine supplementation. BAY2402234 induced DNA damage and S phase arrest by downregulating c‐Myc, CDK2, and cyclin A and upregulating p53 and cyclin E. It also induced caspase‐mediated apoptosis by the upregulation of pro‐apoptotic and downregulation of anti‐apoptotic proteins. Furthermore, BAY2402234 induced caspase‐independent ferroptosis and necroptosis. It decreased phosphorylation of IKK, IκBα, PTEN, Akt, and its downstream targets, suggesting that inhibition of NF‐κB and Akt signaling is involved in its anti‐ATL action. Conclusion: These findings highlight DHODH as a potential therapeutic target for treating ATL. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Inhibitory effect of a neddylation blockade on HTLV-1-infected T cells via modulation of NF-κB, AP-1, and Akt signaling.
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Ishikawa, Chie and Mori, Naoki
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T cells , *MONONUCLEAR leukocytes , *TUMOR suppressor proteins , *ADULT T-cell leukemia , *HTLV-I - Abstract
Adult T-cell leukemia (ATL), caused by HTLV-1, is the most lethal hematological malignancy. NEDD8-activating enzyme (NAE) is a component of the NEDD8 conjunction pathway that regulates cullin-RING ubiquitin ligase (CRL) activity. HTLV-1-infected T cells expressed higher levels of NAE catalytic subunit UBA3 than normal peripheral blood mononuclear cells. NAE1 knockdown inhibited proliferation of HTLV-1-infected T cells. The NAE1 inhibitor MLN4924 suppressed neddylation of cullin and inhibited the CRL-mediated turnover of tumor suppressor proteins. MLN4924 inhibited proliferation of HTLV-1-infected T cells by inducing DNA damage, leading to S phase arrest and caspase-dependent apoptosis. S phase arrest was associated with CDK2 and cyclin A downregulation. MLN4924-induced apoptosis was mediated by the upregulation of pro-apoptotic and downregulation of anti-apoptotic proteins. Furthermore, MLN4924 inhibited NF-κB, AP-1, and Akt signaling pathways and activated JNK. Therefore, neddylation inhibition is an attractive strategy for ATL therapy. Our findings support the use of MLN4924 in ATL clinical trials. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Human T-cell Leukemia Virus Type I Tax Protein Induces the Expression of Anti-Apoptotic Gene Bcl-xL in Human T-Cells through Nuclear Factor-κB and c-AMP Responsive Element Binding Protein Pathways
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Mori, Naoki, Fujii, Masahiro, Cheng, Genhong, Ikeda, Shuichi, Yamasaki, Yoshihiro, Yamada, Yasuaki, Tomonaga, Masao, and Yamamoto, Naoki
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- 2001
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6. The role of CUDC‐907, a dual phosphoinositide‐3 kinase and histone deacetylase inhibitor, in inhibiting proliferation of adult T‐cell leukemia.
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Ishikawa, Chie and Mori, Naoki
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ADULT T-cell leukemia , *PHOSPHOINOSITIDES , *HTLV-I , *HISTONE deacetylase inhibitors , *RETINOBLASTOMA protein , *TREATMENT effectiveness - Abstract
Objectives: New effective therapeutic strategies for human T‐cell leukemia virus type 1 (HTLV‐1)‐driven adult T‐cell leukemia (ATL) are required because of resistance to chemotherapeutic agents. Here, we aimed to determine the therapeutic efficacy of a dual phosphoinositide 3 kinase (PI3K)/histone deacetylase (HDAC) inhibitor, CUDC‐907. Methods: Cell viability, cell cycle progression, and apoptotic events were examined by WST‐8 assay, flow cytometry, and Hoechst 33342 staining. Caspase activity was determined using Calorimetric Caspase Assay kits. Immunoblotting and electrophoretic mobility shift assay were used to assess the intracellular signaling cascades. Results: The combination of PI3K inhibitor BKM120 and HDAC inhibitor LBH589 resulted in a synergistic cytotoxic effect in HTLV‐1‐infected T cells. CUDC‐907 was more efficacious than BKM120 and LBH589. It induced G1 cell cycle arrest with downregulation of cyclin D1/D2, CDK4/6, c‐Myc, and phosphorylated retinoblastoma protein expression. Apoptosis was induced via caspase‐3/8/9 activation along with downregulation of Bcl‐XL, Bcl‐2, XIAP, survivin, and cIAP1/2, and upregulation of Bax and Bak. Histone H3 acetylation, H2AX activation, Hsp27 phosphorylation, and Hsp70 and Hsp27 upregulation were observed after treatment. CUDC‐907 suppressed Akt, NF‐κB, and AP‐1 by downregulating phosphorylated and/or total Akt, IKKα/β, RelA, JunB, and JunD. Conclusion: CUDC‐907 may be a potential therapeutic agent for ATL. [ABSTRACT FROM AUTHOR]
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- 2020
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7. MALT‐1 as a novel therapeutic target for adult T‐cell leukemia.
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Ishikawa, Chie and Mori, Naoki
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ADULT T-cell leukemia , *CELL cycle proteins , *CELL cycle - Abstract
Objectives: T‐cell receptor (TCR) signaling‐induced activation of NF‐κB requires assembly of the CARD11‐BCL10‐MALT‐1 complex and IκB kinase (IKK). Gain‐of‐function alterations in this component of the TCR/NF‐κB pathway are associated with the development of HTLV‐1‐driven adult T‐cell leukemia (ATL). We aimed to determine whether inhibition of MALT‐1‐mediated NF‐κB activation could have anti‐ATL activity. Methods: RT‐PCR, immunoblotting, and electrophoretic mobility shift assays were performed to assess expression levels of MALT‐1 and the intracellular signaling cascades. Cell proliferation, cell cycle progression, and apoptotic events were examined using WST‐8 assays, flow cytometry, and Hoechst 33342 staining. Results: MALT‐1 expression was upregulated in ATL‐derived T‐cell lines compared to that in normal PBMCs and uninfected or HTLV‐1‐transformed T‐cell lines. Targeting MALT‐1 with siRNA decreased cell proliferation. A MALT‐1 inhibitor (MI‐2) suppressed cleavage of the MALT‐1‐target protein, CYLD, and inhibited proliferation via G1 phase arrest. MI‐2 induced apoptosis through caspase‐3/8/9 activation and inhibited the phosphorylation of IKKα/β and IκBα, resulting in the accumulation of IκBα and suppression of NF‐κB‐DNA binding. Additionally, MI‐2 inhibited the expression of apoptosis‐ and cell cycle‐related proteins regulated by NF‐κB. Conclusions: MALT‐1 plays an important regulatory role in NF‐κB signaling during ATL‐genesis, and targeting MALT‐1 is a promising therapeutic strategy for this disease. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Application of Signal Transduction Inhibition as a Therapeutic Strategy for Adult T-Cell Leukemia
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Mori, Naoki
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adult T-cell leukemia ,education ,apoptosis ,humanities ,health care economics and organizations ,signal transduction ,human T-cell leukemia virus type 1 - Abstract
IRPC(International Research Promotion Council:国際研究振興協議会)が授与しているEminent Scientist of the Year 2007(Hematology Oncology and Medical Virology部門)の受賞研究, 再掲:Recent Advances and Research Updates, 9, p.47-58, 2008, 再掲:Austral-Asian Journal of Cancer, Vol.7 No.2, April 2008, [Abstract]Adult T-cell leukemia (ATL) is a fatal malignancy of T lymphocytes caused by human T-cell leukemia virus type 1 (HTLV-1) infection, and remains incurable. Until recently, only a few effective therapies existed. Therefore, continued efforts to develop new therapies are needed. During the last decade, rapid progress has been made in our understanding of the molecular pathways underlying the proliferation of HTLV-1-infected T cells. The processes by which messages initiate protein synthesis, cell cycle progression, and even cell death are transmitted from the cell surface or the cytoplasm to the nucleus are broadly referred to as “signal transduction”. These multistep pathways involve a host of proteins that interact with other proteins in overlapping cascades that flow downstream in a stepwise fashion from the cell membrane to the nucleus. Inappropriate overactivation or underactivation of various components of such signaling pathways can contribute to pathological processes, such as neoplasia. Conversely, molecular and pharmacological interventions that target and attempt to reverse the aberrant state of activation can be potentially of therapeutic benefits. We review the contribution of signal transduction pathways including nuclear factor-kappa B, activator protein-1, janus kinase-signal transducer and activator of transcription, and phosphatidylinositol 3-kinase-Akt to the pathogenesis of ATL and the methods by which targeted inhibition of selected signaling pathway components has been exploited to inhibit the growth of HTLV-1-infected T cells both in vitro and in vivo. The potential translation of such strategies into effects therapies for patients with ATL may improve the poor outcome associated with this neoplasia.
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- 2007
9. シグナル伝達経路の阻害による成人T細胞白血病(ATL)の治療戦略
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Mori, Naoki
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adult T-cell leukemia ,apoptosis ,signal transduction ,human T-cell leukemia virus type 1 - Abstract
IRPC(International Research Promotion Council:国際研究振興協議会)が授与しているEminent Scientist of the Year 2007(Hematology Oncology and Medical Virology部門)の受賞研究, 再掲:Recent Advances and Research Updates, 9, p.47-58, 2008, 再掲:Austral-Asian Journal of Cancer, Vol.7 No.2, April 2008, [Abstract]Adult T-cell leukemia (ATL) is a fatal malignancy of T lymphocytes caused by human T-cell leukemia virus type 1 (HTLV-1) infection, and remains incurable. Until recently, only a few effective therapies existed. Therefore, continued efforts to develop new therapies are needed. During the last decade, rapid progress has been made in our understanding of the molecular pathways underlying the proliferation of HTLV-1-infected T cells. The processes by which messages initiate protein synthesis, cell cycle progression, and even cell death are transmitted from the cell surface or the cytoplasm to the nucleus are broadly referred to as “signal transduction”. These multistep pathways involve a host of proteins that interact with other proteins in overlapping cascades that flow downstream in a stepwise fashion from the cell membrane to the nucleus. Inappropriate overactivation or underactivation of various components of such signaling pathways can contribute to pathological processes, such as neoplasia. Conversely, molecular and pharmacological interventions that target and attempt to reverse the aberrant state of activation can be potentially of therapeutic benefits. We review the contribution of signal transduction pathways including nuclear factor-kappa B, activator protein-1, janus kinase-signal transducer and activator of transcription, and phosphatidylinositol 3-kinase-Akt to the pathogenesis of ATL and the methods by which targeted inhibition of selected signaling pathway components has been exploited to inhibit the growth of HTLV-1-infected T cells both in vitro and in vivo. The potential translation of such strategies into effects therapies for patients with ATL may improve the poor outcome associated with this neoplasia., 論文
- Published
- 2007
10. Effects of NVP-BEZ235, a dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor, on HTLV-1-infected T-cell lines.
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Ishikawa, Chie, Senba, Masachika, and Mori, Naoki
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ADULT T-cell leukemia ,HTLV-I ,RAPAMYCIN ,PHOSPHATIDYLINOSITOL 3-kinases ,CANCER cell growth ,LEUKEMIA treatment ,PREVENTION - Abstract
Adult T-cell leukemia (ATL) is an aggressive type of malignancy caused by human T-cell leukemia virus type 1 (HTLV-1). In ATL, the phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway is constitutively active, promoting cell proliferation, survival and chemoresistance. Thus, the PI3K signaling pathway is an attractive therapeutic target for ATL. In the present study, the effects of RAD001 (an mTOR inhibitor), NVP-BKM120 (a pan-PI3K inhibitor) and NVP-BEZ235 (a novel dual PI3K/mTOR inhibitor) on cultured HTLV-1-infected T-cell lines were compared. The results demonstrated that NVP-BEZ235 was more efficacious compared with RAD001 and NVP-BKM120 at inhibiting cell growth. NVP-BEZ235 exhibited cytostatic rather than cytotoxic effects on various HTLV-1-infected T-cell lines, where it induced cell cycle arrest at G1 phase. NVP-BEZ235 downregulated cyclin D1, cyclin D2, cyclin E, cyclin dependent kinase (CDK)2 and CDK4 expression, and the phosphorylation of retinoblastoma protein. In C.B-17/Icr-severe combined immune deficiency mice implanted with HTLV-1-infected HUT-102 cells, oral NVP-BEZ235 caused marked retardation of tumor growth compared with the control. The present in vitro and in vivo studies highlight the efficacious dual inhibition of PI3K, and mTOR following NVP-BEZ235 treatment. Thus, the results of the current study provide preclinical rationale for phase I clinical studies to examine the effects of NVP-BEZ235 in patients with ATL. [ABSTRACT FROM AUTHOR]
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- 2018
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11. Expression and significance of Pim-3 kinase in adult T-cell leukemia.
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Ishikawa, Chie, Senba, Masachika, Hashimoto, Tadashi, Imaizumi, Atsushi, and Mori, Naoki
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ADULT T-cell leukemia ,WESTERN immunoblotting ,SYNTAXINS ,SERINE ,THREONINE - Abstract
Background Human T-cell leukemia virus type 1 ( HTLV-1) causes adult T-cell leukemia ( ATL). Viral Tax protein plays a major role in ATL development. Pim family of serine/threonine kinases is composed of Pim-1, -2, and -3. The potential of Pim family as a target in ATL was analyzed. Methods RT- PCR and Western blotting were used to determine the expression of Pim kinases, Tax, and intracellular signal molecules. Knockdown of Pim-3 and RelA was performed using small interfering RNA. The effects on cell proliferation, viability, cell cycle, and apoptosis were analyzed by WST-8, propidium iodide, and APO2.7 assay. NF-κB DNA binding activity was investigated by electrophoretic mobility shift assay. Results Pim-3 expression was restricted to HTLV-1-infected T-cell lines. Tax induced Pim-3 expression through NF-κB. Knockdown of Pim-3 showed growth inhibition of HTLV-1-infected T cells. NJC97- NH, a novel inhibitor of the Pim-1/3 kinases, inhibited cell viability. NJC97- NH induced G2/M cell cycle arrest associated with downregulation of cyclin A and cyclin B1 expression, as well as apoptosis accompanied with downregulation of XIAP and Mcl-1 expression through inhibition of NF-κB pathway, mediated through decrease in IκBα and RelA phosphorylation. Conclusion Pim-3 is a potentially suitable target for the development of novel therapeutic agents against ATL. [ABSTRACT FROM AUTHOR]
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- 2017
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12. The antipsychotic drug pimozide is effective against human T-cell leukemia virus type 1-infected T cells.
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Ishikawa, Chie and Mori, Naoki
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HTLV , *T cells , *ENDOPLASMIC reticulum , *HTLV-I , *ADULT T-cell leukemia , *DOPAMINE receptors , *LABORATORY mice - Abstract
Patients with adult T-cell leukemia (ATL), caused by the human T-cell leukemia virus type 1 (HTLV-1), exhibit poor prognosis owing to drug resistance. Pimozide is a dopamine D2 receptor antagonist and antipsychotic shown to exhibit anticancer activity. Herein, we investigated whether pimozide exerts anti-ATL effects and explored the mechanisms underlying these effects. Pimozide inhibited cell growth and survival in HTLV-1-infected T cells but not in the uninfected T cells. The dopamine D2 receptor subfamily mRNA expression levels in HTLV-1-infected T cells were high. Pimozide induced G1 cell cycle arrest concomitant with the upregulation of p21/p27/p53, and suppression of cyclin D2/E, cyclin-dependent kinase 2/4/6 and c-Myc expression, and pRb phosphorylation. Pimozide also induced apoptosis by activating caspases, upregulating pro-apoptotic proteins and downregulating anti-apoptotic proteins. Additionally, it promoted reactive oxygen species (ROS) generation and increased the expression of the endoplasmic reticulum stress marker activating transcription factor 4 and the DNA damage-inducible protein GADD45α and the phosphorylation of the DNA damage marker H2AX. Furthermore, pimozide-induced cytotoxicity was partially inhibited by a ROS scavenger, and pan-caspase and necroptosis inhibitors, indicating the involvement of caspase-dependent and -independent lethal pathways. The activities of the nuclear factor-κB, Akt, STAT3/5 and AP-1 signaling pathways were inhibited via the dephosphorylation of IκBα, IκB kinase α/β, Akt and STAT3/5, in addition to reduced JunB and JunD expression in HTLV-1-infected T cells. Pimozide also exhibited potent anti-ATL activity in the xenograft mouse model. These findings demonstrated the efficacy of pimozide as a potential therapeutic agent for ATL. [ABSTRACT FROM AUTHOR]
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- 2021
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13. Elevated expression of CD30 in adult T-cell leukemia cell lines: possible role in constitutive NF-κB activation.
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Higuchi, Masaya, Matsuda, Takehiro, Mori, Naoki, Yamada, Yasuaki, Horie, Ryouichi, Watanabe, Toshiki, Takahashi, Masahiko, Oie, Masayasu, and Fujii, Masahiro
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ADULT T-cell leukemia ,HTLV diseases ,CELL culture ,CELL lines ,CELL death ,GENETIC engineering - Abstract
Background: Human T-cell leukemia virus type 1 (HTLV-1) is associated with the development of adult T-cell leukemia (ATL). HTLV-1 encoded Tax1 oncoprotein activates the transcription of genes involved in cell growth and anti-apoptosis through the NF-κB pathway, and is thought to play a critical role in the pathogenesis of ATL. While Tax1 expression is usually lost or minimal in ATL cells, these cells still show high constitutive NF-κB activity, indicating that genetic or epigenetic changes in ATL cells induce activation independent of Tax1. The aim of this study was to identify the molecules responsible for the constitutive activation of NF-κB in ATL cells using a retroviral functional cloning strategy. Results: Using enhanced green fluorescent protein (EGFP) expression and blasticidin-resistance as selection markers, several retroviral cDNA clones exhibiting constitutive NF-κB activity in Rat-1 cells, including full-length CD30, were obtained from an ATL cell line. Exogenous stable expression of CD30 in Rat-1 cells constitutively activated NF-κB. Elevated expression of CD30 was identified in all ATL lines examined, and primary ATL cells from a small number of patients (8 out of 66 cases). Conclusion: Elevated CD30 expression is considered one of the causes of constitutive NF-κB activation in ATL cells, and may be involved in ATL development. [ABSTRACT FROM AUTHOR]
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- 2005
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14. Multiple γc-receptor expression in adult T-cell leukemia.
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Baba, Hirofumi, Yamada, Yasuaki, Mori, Naoki, Hayashibara, Toshihisa, Harasawa, Hitomi, Tsuruda, Kazuto, Sugahara, Kazuyuki, Soda, Hiroshi, Takasaki, Yumi, Tawara, Masayuki, Hirakata, Yoichi, Tomonaga, Masao, and Kamihira, Shimeru
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ADULT T-cell leukemia ,HTLV ,INTERLEUKINS - Abstract
Abstract: Constitutive expression of the IL-2 receptor (IL-2R) on adult T-cell leukemia (ATL) cells and the presence of permanent IL-2-dependent ATL cell lines indicate that the signal transduction system via IL-2R is a key element for the development of this disease. IL-2R is a member of the common γ-chain (γc)-receptor family and shares γ with IL-4R, IL-7R, IL-9R, and IL-15R. In addition to IL-2R, ATL cells express IL-15R and respond to IL-15. In the present study, we examined other members of this receptor family. ATL cells showed various levels of IL-4Rα (CD124) and IL-7Rα (CD127) expression, and responded to these cytokines. In contrast, ATL cells hardly responded to IL-9. As primary samples were a mixed population and the results may have been modified by contaminating normal cells, we used ATL cell lines as pure ATL cell populations. Here, we report that IL-2-dependent ATL cell lines also express IL-4Rα and respond to IL-4, which was verified by the activation of cytoplasmic transcriptional activator Stat6 protein. Moreover, a novel ATL cell line that grows stably in an IL-7-dependent manner was established from one of the cell lines, and IL-7 induced Stat5 activation in this cell line. These results indicated that ATL cells have the potential to express all γc-receptors except IL-9R. Overlapping and switching of cytokine receptors supported the idea that ATL cells can rapidly select the appropriate γc-receptor according to conditions. [ABSTRACT FROM AUTHOR]
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- 2002
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15. Matrix metalloproteinase-9 and vascular endothelial growth factor: a possible link in adult T-cell leukaemia cell invasion.
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Hayashibara, Toshihisa, Yamada, Yasuaki, Onimaru, Yasuyuki, Tsutsumi, Chizuko, Nakayama, Susumu, Mori, Naoki, Miyanishi, Takayuki, Kamihira, Shimeru, Tomonaga, Masao, and Maita, Tetsuo
- Subjects
ADULT T-cell leukemia ,METALLOPROTEINASES ,MESSENGER RNA - Abstract
Summary. Plasma from a total of 57 patients with adult T-cell leukaemia (ATL) (acute ATL, 39 patients; lymphoma ATL, one patient; chronic ATL, 15 patients; smouldering ATL, two patients) and 20 healthy controls was analysed for the presence of type IV gelatinase activity with clinical features. A significant elevation of plasma matrix metalloproteinase-9 (MMP-9) was observed in some ATL patients, particularly in the patients with malignant cell infiltration. MMP-9 was found to be secreted into the conditioned medium from all ATL cell lines examined. Moreover, the corresponding mRNA was detectable both in all ATL cell lines examined and in the majority of primary acute ATL cells, indicating that ATL cells are capable of synthesizing and secreting MMP-9. We previously demonstrated that a high incidence of ATL cell infiltration was closely related to a high plasma level of vascular endothelial growth factor (VEGF) produced by ATL cells themselves. This present study showed that the presence of increased plasma MMP-9 was closely associated with elevated plasma VEGF in ATL patients. Furthermore, we showed that both increased plasma MMP-9 and VEGF were significantly related to high ATL cell infiltration. All these findings strongly suggest that MMP-9 and VEGF act co-operatively in the process of ATL cell invasion. [ABSTRACT FROM AUTHOR]
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- 2002
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16. Lactacystin activates FLICE (caspase 8) protease and induces apoptosis in Fas-resistant adult T-cell leukemia cell lines.
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Yamada, Yasuaki, Sugahara, Kazuyuki, Tsuruda, Kazuto, Nohda, Kazuo, Mori, Naoki, Hata, Tomoki, Maeda, Takahiro, Hayashibara, Toshihisa, Joh, Tatsuroh, Honda, Miyuki, Tawara, Masayuki, Tomonaga, Masao, Miyazaki, Yoshitsugu, and Kamihira, Shimeru
- Subjects
APOPTOSIS ,ADULT T-cell leukemia - Abstract
Abstract: Lactacystin (LC) is a specific inhibitor of the proteasome, and has recently been shown to induce apoptosis in certain cell lines. In the present study, we established Fas-resistant adult T-cell leukemia (ATL) cell subclones RSO4 and RST1 from their parental Fas-sensitive cell lines SO4 and ST1, and examined whether LC can overcome Fas resistance. LC completely inhibited proteasome function as determined by a peptidyl-MCA substrate (LLVY-MCA and LLE-MCA), and induced apoptosis in these cell lines irrespective of Fas sensitivity at low concentrations (∼ 10 μM). LC induced the activation of caspase 3 (CPP32/Yama) and caspase 6 proteases in an identical manner to Fas-mediated apoptosis. Moreover, LC induced the activation of caspase 8 (FLICE) protease, which is the initiator of the Fas-mediated apoptotic cascade. Synthesized proteasome inhibitory peptide MG-115 (ZLLnV-CHO) also induced apoptosis in these cell lines. These results indicated that proteasome inhibitors overcome Fas-resistance by bypassing the proximal part of the Fas signal. Inhibition of the proteasome function may be a new strategy for the treatment of ATL. [ABSTRACT FROM AUTHOR]
- Published
- 2000
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17. Interleukin-10 Gene Expression and Adult T-Cell Leukemia.
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Mori, Naoki
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ADULT T-cell leukemia , *INTERLEUKIN-10 - Abstract
Examines clinical specimens of patients with adult T-cell leukemia (ATL) for interleukin 10 (IL-10) expression. Comparison of IL-10 levels in ATL patients and healthy subjects; Transcriptional regulation of IL-10 gene expression.
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- 1998
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18. Honokiol induces cell cycle arrest and apoptosis via inhibition of survival signals in adult T-cell leukemia
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Ishikawa, Chie, Arbiser, Jack L., and Mori, Naoki
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CELL cycle regulation , *ADULT T-cell leukemia , *COLORIMETRY , *PROPIDIUM iodide , *APOPTOSIS , *WESTERN immunoblotting , *GENE expression - Abstract
Abstract: Background: Honokiol, a naturally occurring biphenyl, possesses anti-neoplastic properties. We investigated activities of honokiol against adult T-cell leukemia (ATL) associated with human T-cell leukemia virus type 1 (HTLV-1). Methods: Cell viability was assessed using colorimetric assay. Propidium iodide staining was performed to determine cell cycle phase. Apoptotic effects were evaluated by 7A6 detection and caspases activity. Expressions of cell cycle- and apoptosis-associated proteins were analyzed by Western blot. We investigated the efficacy of honokiol in mice harboring tumors of HTLV-1-infected T-cell origin. Results: Honokiol exhibited cytotoxic activity against HTLV-1-infected T-cell lines and ATL cells. We identified two different effects of honokiol on HTLV-1-infected T-cell lines: cell cycle inhibition and induction of apoptosis. Honokiol induced G1 cell cycle arrest by reducing the expression of cyclins D1, D2, E, CDK2, CDK4, CDK6 and c-Myc, while apoptosis was induced via reduced expression of cIAP-2, XIAP and survivin. The induced apoptosis was also associated with activation of caspases-3 and -9. In addition, honokiol suppressed the phosphorylation of IκBα, IKKα, IKKβ, STAT3, STAT5 and Akt, down-regulated JunB and JunD, and inhibited DNA binding of NF-κB, AP-1, STAT3 and STAT5. These effects resulted in the inactivation of survival signals including NF-κB, AP-1, STATs and Akt. Honokiol was highly effective against ATL in mice Conclusions: Our data suggested that honokiol is a systemically available, non-toxic inhibitor of ATL cell growth that should be examined for potential clinical application. General significance: Our findings provide a rationale for clinical evaluation of honokiol for the management of ATL. [Copyright &y& Elsevier]
- Published
- 2012
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19. Pathogenesis of Metastatic Calcification and Acute Pancreatitis in Adult T-Cell Leukemia under Hypercalcemic State.
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Senba, Masachika, Kawai, Kioko, and Mori, Naoki
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PANCREATITIS , *METASTASIS , *CALCIFICATION , *ADULT T-cell leukemia , *HTLV-I , *PARATHYROID hormone-related protein - Abstract
Human T-cell leukemia virus type-1 (HTLV-1) is the causative agent of adult T-cell leukemia (ATL). Hypercalcemia is common in patients with ATL. These patients rarely develop metastatic calcification and acute pancreatitis. The underlying pathogenesis of this condition is osteoclast hyperactivity with associated overproduction of parathyroid hormone-related protein, which results in hypercalcemia in association with bone demineralization. The discovery of the osteoclast differentiation factor receptor activator of nuclear factor-κB ligand (RANKL), its receptor RANK, and its decoy receptor osteoprotegerin (OPG), enhanced our understanding of the mechanisms of ATL-associated hypercalcemia. Macrophage inflammatory protein-1-a, tumor necrosis factor-a, interleukin-1, and interleukin-6 are important molecules that enhance the migration and differentiation of osteoclasts and the associated enhanced production of RANKL for osteoblast formation. In this paper, we focus on metastatic calcification and acute pancreatitis in ATL, highlighting recent advances in the understanding of the molecular role of the RANKL/RANK/OPG system including its interaction with various cytokines and calciotropic hormones in the regulation of osteoclastogenesis for bone resorption in hypercalcemic ATL patients. [ABSTRACT FROM AUTHOR]
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- 2012
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20. Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL
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Hayashibara, Toshihisa, Yamada, Yasuaki, Mori, Naoki, Harasawa, Hitomi, Sugahara, Kazuyuki, Miyanishi, Takayuki, Kamihira, Shimeru, and Tomonaga, Masao
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HTLV-I , *ADULT T-cell leukemia , *CELL receptors - Abstract
Human T-cell leukemia virus type 1 is the etiologic agent of adult T-cell leukemia (ATL), although the precise mechanism involved in the transformation process has not yet been defined. The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that can influence cell proliferation and differentiation. We investigated the expression and activation of AhR in ATL. RT-PCR and Western blot analyses showed high expression levels of AhR in ATL cell lines. The elevated expression of AhR was in part attributable to the action of the viral transactivator protein, Tax. Interestingly, activation of the AhR was found in ATL cell lines in the absence of apparent exogenous ligands. Importantly, the increased expression and activation of AhR were also observed in some primary ATL cells. To our best knowledge, this is the first report to show the lymphoid malignancy having constitutive activation of AhR. A possible link between increased AhR expression and leukemogenesis in ATL is discussed. [Copyright &y& Elsevier]
- Published
- 2003
- Full Text
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21. Evaluation of artesunate for the treatment of adult T-cell leukemia/lymphoma.
- Author
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Ishikawa, Chie, Senba, Masachika, and Mori, Naoki
- Subjects
- *
ADULT T-cell leukemia , *HTLV-I , *CELL cycle , *RITUXIMAB , *IRON chelates - Abstract
Adult T-cell leukemia/lymphoma (ATLL) is an aggressive disease caused by infection with human T-cell leukemia virus type 1 (HTLV-1). Successful treatment is limited by resistance to chemotherapies. Therefore, there is an urgent need to develop novel effective strategies. Artesunate (ART), a widely used antimalarial compound, has been shown to exert cytotoxicity. Here, we aimed to assess the anti-ATLL activities of ART and to elucidate the possible molecular mechanisms involved in this effect. Compared with uninfected T cells, HTLV-1-infected T-cell lines were sensitive to ART-induced cytotoxicity. ART caused cell cycle arrest at G 1 and/or G 2 /M phases, which was associated with decreased expression of cyclin dependent kinase 1/2/4/6, cyclin B1/D2/E and c-Myc, and increased expression of p21. ART-induced apoptosis corresponded to activation of caspase-8/9/3; decreased expression of Bcl-xL, Bcl-2, myeloid cell leukemia-1, survivin, X-linked inhibitor of apoptosis protein and cellular inhibitor of apoptosis 1/2; and increased expression of Bak. ART increased intracellular reactive oxygen species and activation of the DNA damage marker γ-H2AX. Moreover, ART-induced cytotoxicity was partly reversed by treatment with a reactive oxygen species scavenger, iron chelator, and necroptosis or ferroptosis inhibitor, suggesting the involvement of caspase-dependent and -independent lethal pathways. These effects were correlated with inhibition of nuclear factor-κB and activator protein-1 signaling through dephosphorylation of IκBα, IκB kinase (IKK) α and IKKβ, and decreased expression of JunB and JunD. Importantly, intraperitoneal injection with ART lowered tumor burden in an ATLL murine model. These preclinical results provide a rationale for evaluating the efficacy of ART in patients with ATLL. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
22. CD69 overexpression by human T-cell leukemia virus type 1 Tax transactivation.
- Author
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Ishikawa, Chie, Kawakami, Hirochika, Uchihara, Jun-Nosuke, Senba, Masachika, and Mori, Naoki
- Subjects
- *
HTLV-I , *ADULT T-cell leukemia , *LYMPHOCYTES , *DEACETYLATION , *METHYLATION , *LYMPH nodes - Abstract
Abstract: Human T-cell leukemia virus type 1 (HTLV-1) infection is associated with the development of adult T-cell leukemia (ATL) and various inflammatory diseases. CD69 is a marker of early activation of lymphocytes. We investigated the effects of HTLV-1 infection on the expression of CD69. The CD69 gene was upregulated in all viral protein Tax-expressing HTLV-1-transformed T-cell lines, except MT-2 and peripheral blood mononuclear cells from patients with ATL compared with uninfected T-cell line, Tax-negative ATL-derived T-cell lines and normal peripheral blood mononuclear cells. Flow cytometric analysis and immunohistochemical analysis confirmed the enhanced expression of CD69 in HTLV-1-transformed T-cell lines and in ATL cells in lymph nodes and skin lesions, and its absence in MT-2 and peripheral blood mononuclear cells. CD69 expression was induced following infection of human T-cell line with HTLV-1, and specifically by Tax. Tax transcriptionally activated CD69 gene through both nuclear factor-κB and cyclic adenosine 3′,5′-monophosphate response element-binding protein signaling pathways. Detailed analysis of the CD69 promoter indicated that the Tax-induced expression of CD69 was regulated by multiple cis-acting elements and by the interplay of transcription factors of the nuclear factor-κB, early growth response and cyclic adenosine 3′,5′-monophosphate response element-binding protein families. The lack of CD69 expression in MT-2 is due to epigenetic mechanism involving deacetylation, but not methylation. We conclude that CD69 is a Tax-regulated gene, and its regulation by Tax may play a role in cellular activation and HTLV-1-induced disease pathogenesis. [Copyright &y& Elsevier]
- Published
- 2013
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- View/download PDF
23. Targeting Bcl-2 family proteins in adult T-cell leukemia/lymphoma: In vitro and in vivo effects of the novel Bcl-2 family inhibitor ABT-737
- Author
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Ishitsuka, Kenji, Kunami, Naoko, Katsuya, Hiroo, Nogami, Rumiko, Ishikawa, Chie, Yotsumoto, Fusanori, Tanji, Hiroe, Mori, Naoki, Takeshita, Morishige, Miyamoto, Shingo, and Tamura, Kazuo
- Subjects
- *
ADULT T-cell leukemia , *TARGETED drug delivery , *T-cell lymphoma , *ENZYME inhibitors , *APOPTOSIS , *ANTINEOPLASTIC agents , *CELL lines , *LABORATORY mice , *TUMOR growth , *CELL-mediated cytotoxicity - Abstract
Abstract: Adult T-cell leukemia/lymphoma (ATLL) is a peripheral T-cell malignancy caused by human T-lymphotropic virus type I (HTLV-1). ABT-737, a small molecule inhibitor of Bcl-2, Bcl-XL, and Bcl-w, significantly induced apoptosis in HTLV-1 infected T-cell lines as well as in fresh ATLL cells, and synergistically enhanced the cytotoxicity and apoptosis induced by conventional cytotoxic drugs. Moreover, ABT-737 significantly inhibited the in vivo tumor growth of an ATLL mouse model. These results suggest that the use of an agent targeting anti-apoptotic bcl-2 family proteins, either alone or in combination with other conventional drugs, represents a novel promising approach for ATLL. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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24. Effects of hippuristanol, an inhibitor of eIF4A, on adult T-cell leukemia
- Author
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Tsumuraya, Tomoyuki, Ishikawa, Chie, Machijima, Yoshiaki, Nakachi, Sawako, Senba, Masachika, Tanaka, Junichi, and Mori, Naoki
- Subjects
- *
CHEMICAL inhibitors , *ADULT T-cell leukemia , *CANCER cell proliferation , *NF-kappa B , *STEROIDS , *TUMOR growth , *PREVENTION - Abstract
Abstract: We evaluated the anti-adult T-cell leukemia (ATL) effects of hippuristanol, an eukaryotic translation initiation inhibitor from the coral Isis hippuris. Hippuristanol inhibited proliferation of HTLV-1-infected T-cell lines and ATL cells, but not normal peripheral blood mononuclear cells. It induced cell cycle arrest during G1 phase by reducing the expression of cyclin D1, cyclin D2, CDK4 and CDK6, and induced apoptosis by reducing the expression of Bcl-xL, c-IAP2, XIAP and c-FLIP. The induced apoptosis was associated with activation of caspase-3, -8 and -9. Hippuristanol also suppressed IkappaBalpha phosphorylation and depleted IKKalpha, IKKgamma, JunB and JunD, resulting in inactivation of NF-kappaB and AP-1. It also suppressed carbonic anhydrase type II expression. In addition to its in vitro effects, hippuristanol suppressed tumor growth in mice with severe combined immunodeficiency harboring tumors induced by inoculation of HTLV-1-infected T cells. These preclinical data suggest that hippuristanol could be a potentially useful therapeutic agent for patients with ATL. [Copyright &y& Elsevier]
- Published
- 2011
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25. Curcumin suppresses constitutive activation of AP-1 by downregulation of JunD protein in HTLV-1-infected T-cell lines
- Author
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Tomita, Mariko, Kawakami, Hirochika, Uchihara, Jun-nosuke, Okudaira, Taeko, Masuda, Masato, Takasu, Nobuyuki, Matsuda, Takehiro, Ohta, Takao, Tanaka, Yuetsu, and Mori, Naoki
- Subjects
- *
HTLV-I infections , *T cells , *CELL lines , *ADULT T-cell leukemia - Abstract
Abstract: Activation of the activator protein 1 (AP-1) plays a critical role in oncogenesis by human T-cell leukemia virus type 1 (HTLV-1), the etiologic agent of adult T-cell leukemia (ATL), and is required for maintenance of the malignant phenotype. Curcumin (diferuloylmethane), the major pigment of the spice turmeric, has anti-tumor activity; however, the effect of curcumin against ATL has not been elucidated. In this study, we examined the effects of curcumin on AP-1 activity in HTLV-1-infected T-cell lines. Curcumin suppressed the constitutive AP-1 DNA-binding and transcriptional activity in HTLV-1-infected T-cell line. Curcumin also inhibited HTLV-1 Tax-induced AP-1 transcriptional activity. JunD was detectable as a major component of the AP-1–DNA complex in HTLV-1-infected T-cell lines using the supershift assay. The expression of JunD was suppressed by curcumin treatment. Curcumin inhibited the growth of HTLV-1-infected T-cell lines by inducing cell cycle arrest followed by apoptosis. Our results suggest that suppression of the constitutively active AP-1 by curcumin is due to, at least in-part, reducing the expression of JunD by curcumin. Inhibition of AP-1 activity by curcumin may be one of the mechanisms responsible for the anti-ATL effect of curcumin. We propose that curcumin is a potentially promising compound for the treatment of ATL. [Copyright &y& Elsevier]
- Published
- 2006
- Full Text
- View/download PDF
26. Methylation analysis of the adenomatous polyposis coli (APC) gene in adult T-cell leukemia/lymphoma
- Author
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Yang, Yang, Takeuchi, Seisho, Tsukasaki, Kunihiro, Yamada, Yasuaki, Hata, Tomoko, Mori, Naoki, Fukushima, Atsuki, Seo, Hiromi, Koeffler, H. Phillip, and Taguchi, Hirokuni
- Subjects
- *
ADULT T-cell leukemia , *LYMPHOMAS , *METHYLATION , *LEUKEMIA - Abstract
We investigated methylation status of the adenomatous polyposis coli (APC) gene in adult T-cell leukemia/lymphoma (ATL). APC methylation was found in 15 of 31 (48%) primary samples, and 2 of 4 (50%) ATL cell lines. Methylation of the APC gene occurred more frequently in acute ATL (12/21) (57%) than chronic ATL (1/8) (13%) (P = 0.03). APC was not expressed in the APC-methylated ATL cell line ST1. Demethylation with 5-azacytidine treatment restored APC expression in the ST1 cell line. Our data show that hypermethylation of the APC gene is involved in the pathogenesis of ATL. [Copyright &y& Elsevier]
- Published
- 2005
- Full Text
- View/download PDF
27. Human T-cell leukemia virus type 1 Tax oncoprotein induces and interacts with a multi-PDZ domain protein, MAGI-3
- Author
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Ohashi, Minako, Sakurai, Mamoru, Higuchi, Masaya, Mori, Naoki, Fukushi, Masaya, Oie, Masayasu, Coffey, Robert J., Yoshiura, Kenta, Tanaka, Yuetsu, Uchiyama, Makoto, Hatanaka, Masakazu, and Fujii, Masahiro
- Subjects
- *
EPIPHANY , *LEUCOCYTOSIS , *PRELEUKEMIA , *ANEMIA - Abstract
Human T-cell leukemia virus type 1 (HTLV-1) is the causative agent of adult T-cell leukemia (ATL), whereas the closely related virus HTLV-2 has not been associated with such malignant conditions. HTLV-1 Tax1 oncoprotein transforms a rat fibroblast cell line (Rat-1) much more efficiently than does HTLV-2 Tax2. By using a differential display analysis, we isolated MAGI-3 as a Tax1-inducible gene in Rat-1 cells. Reverse transcription-polymerase chain reaction (RT-PCR) analysis confirmed that Tax1 induced MAGI-3 in Rat-1 cells. MAGI-3 has multiple PDZ domains and interacted with Tax1 but not Tax2 in 293T cells. The interaction of Tax1 with MAGI-3 was dependent on a PDZ domain-binding motif, which is missing in Tax2. The interaction of Tax1 with MAGI-3 altered their respective subcellular localization, and moreover, the interaction correlated well with the high transforming activities of Tax1 in Rat-1 cells relative to Tax2. MAGI-3 mRNA and the allied MAGI-1, but not MAGI-2, were expressed in HTLV-1-infected T-cell lines. Our results suggest that the interaction of Tax1 and MAGI-3 alters their respective biological activities, which may play a role in transformation by Tax1 as well as in the pathogenesis of HTLV-1-associated diseases. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
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