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To study the expression of p-STAT3 and PTEN in human laryngeal squamous carcinoma, to explore their relations and clinical significance.Formalin-fixed and paraffin-embedded tissues from 67 cases of laryngeal squamous carcinoma, 25 cases of normal mucosa over 2.0 cm away from tumor margin in 25 patients with total or subtotal laryngectomy were evaluated for the expression of p-STAT3, PTEN by SP immunohistochemistry, the levels of these proteins in tissues and their correlation with clinicopathological parameters of laryngeal squamous carcinoma were analyzed. The prognostic analysis was performed by Kaplan-Merier.The expression rates of p-STAT3 protein in laryngeal squamous carcinoma and normal control laryngeal mucous tissues were 71.64%, 16% respectively. There was significant difference between them (Zc = 4.7052, P0.01); The expression rates of PTEN protein in laryngeal squamous carcinoma and normal control laryngeal mucous tissues were 41.79%, 96% respectively. There was significant difference between them (Zc = 5.7037, P0.01); The expressions of p-STAT3 and PTEN in laryngeal squamous carcinoma were associated with clinical stage, differentiation grade, lymph nodal metastases and prognosis (P0.01). There was a negative correlation between the expression of p-STAT3 and PTEN,and their correlation coefficient was r = -0.5148 (P0.01). p-STAT3 positive expression rate in patients survived over a 5 years follow up was 56.25% (18/32), which was obviously lower than the rate 82.35% (14/17) in those dead.The expression of p-STAT3 and PTEN may take important roles in the tumorigenesis, aggressiveness, metastases and prognosis of laryngeal squamous carcinoma. The high expression of p-STAT3 was negatively correlated with the lower PTEN in laryngeal squamous carcinoma, which suggested that PTEN may be a downstream target gene of p-STAT3.

To investigate the role and clinical value of the serum level of hepatocyte growth factor (HGF) and vascular endothelial growth factor(VEGF) in laryngeal squamous cell cancer and the relationship between HGF, VEGF and clinicopathological factors.We measured serum HGF and VEGF level in 54 laryngeal squamous cell cancer patients using ELISA to demonstrate the variation of HGF, VEGF level before operation and at day 1, 3, 7, 14 post-surgery. The concentration of the serum HGF, VEGF content in 35 normal healthy people and in 30 vocal cords polyps patients were measured by the quantitative sandwich enzyme linked immunoassay technique.(1) The level of serum HGF and VEGF in the patients with laryngeal squamous cell cancer was significantly higher than that of normal healthy people and vocal cord polyps patients (P0.01). (2) The level of serum HGF, VEGF in the patients with laryngeal squamous cell cancer was related to clinical stage, differentiation situation, lymph nodes metastasis (P0.01), but it was independent of age at diagnosis, gender and tumor location (P0.05). (3) There was a significantly positive correlation between preoperative serum VEGF and HGF levels (r = 0.7667, P0.01). (4) Post-operational serum HGF levels in 54 laryngeal squamous cell cancer patients who underwent surgical intervention increased significantly, peaked at day 3 after operation. Serum HGF levels of survivors during follow-up period gradually decreased at day 7 and day 14 after operation. The postoperative serum levels of VEGF were decreased significantly than that of preoperation.HGF and VEGF may play an important role in the development and progression of human laryngeal cancer. Elevated serum HGF and VEGF levels predict a more aggressive biological behavior in laryngeal squamous cell cancer.

To investigate the expression and clinical significance of Skp2 and PTEN in glottic carcinoma and the relationship between the two genes.Formalin-fixed and paraffin-embedded tissues, which came from 42 cases of glottic carcinoma and 16 cases of atypical hyperplasia of vocal fold and 27 cases of vocal cord polyp, were detected for the expression of Skp2, PTEN by SP immunohistochemistry, then we analyzed the result statistically.The expression rates of Skp2 protein in vocal cord polyp, atypical hyperplasia of vocal cord and glottic carcinoma were 11.11%, 37.50%, 40.48% respectively. There was significant difference among them (P0.01); the expression rates of PTEN protein in vocal cord polyp, atypical hyperplasia of vocal cord and glottic carcinoma were 100.00%, 75.00%, 52.38% respectively. There was significant difference among them (P0.05), the expressions of Skp2 and PTEN in glottic carcinoma were associated with clinical stage, lymph nodal metastases and prognosis (P0.05); there was a negative correlation between the expression of Skp2 and PTEN, and their correlation coefficient was r= -0.4301 (P0.01).The expressions of Skp2 and PTEN may play an important roles in the tumorigenesis, metastases and poor prognosis of glottic carcinoma. These changes may be the early molecular event of the carcinogenesis. The high expression of Skp2 was negative correlation with the lower PTEN in glottic carcinoma.