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The total of 54 patients with chronic periodontitis of different severity was tested using real-time PCR (Dentoflor kit). The group included 38 patients with chronic gastritis. For the first time, a higher prevalence of Treponema denticola in periodontium of males in comparison with females was demonstrated. The patients with chronic gastritis had more human genome DNA at their periodontium than healthy individuals. Non-parametric statistical analysis demonstrated high association of periodontium colonization with. T. forsythensis and T. denticola (but not Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia) with the severity of the chronic periodontitis.

Biological material of 92 patients (18-85 years old) with different severity chronic periodontitis were analyzed for bacterial pathogen colonization by using Dentofol kit (DNA-technology, Moscow). The cohort included 70 individuals with chronic gastritis, 2 patients with gastric and duodenal ulceration and 20 individuals with no gastric/duodenal pathology. The tight- est association with severity of the chronic periodontitis in the analyzed sub-cohort with the chronic gastritis was found with the prevalence of a complex T. for sythensis/T. denticola. Key contribution of this complex to progression of periodon- titis in males of the eldest group (above 55) was hypothesized. This data essentially differ from published results of other research groups where T. forsythensis and T. denticola were never reported as the principal causative agents of the chronic periodonitis in the gender/age/combined pathology normalized cohorts.

Real-time quantitative PCR (Dentofl or kit) was used to detect DNA of periodontal pathogens in specimens from 92 patients with chronic periodontitis and from a control sample of 12 normal subjects. A bimodal distribution of patients by periodontium colonization with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythensis, and T. denticola was demonstrated. A new approach to interpretation of the results of quantitative evaluation of periodontal pathogens, including the notion of pathological colonization level, led to classification of all cases with chronic generalized periodontitis into 3 groups: associated with A. actinomycetemcomitans, with T. forsythensis/T. denticola complex, and cases of uncertain genesis.

We compared efficacy of combined treatment of males with anogenital warts given intramuscular cycloferon or cycloferon electrophoresis. Patients with manifest HPV infection (n=107) were divided into two groups. Group 1 (n=72) received intramuscular cycloferon (2 ml 12.5% solution each other day for 10 days), group 2 (n=35) received cycloferon electrophoresis on genital region (2 ml 12.5% solution daily in 1-2 days, 5 injections). Measurements were made of local cytokines in urethral secretion, IFN-alpha, IFN-gamma, lactoferrin, sexual hormones (estradiol and testosterone) in blood serum before and after treatment. Focal immunophysiotherapy with cycloferon in combined treatment of males with anogenital warts vs intramuscular cycloferon treatment reduces recurrence rate 2 times, has no negative effect on serum level of sexual hormones, a course dose and duration of immunotherapy decreased 2-fold, is well tolerated, is not painful. Cycloferon electrophoresis has a more potent effect on improvement of local immunity. Thus, local immunophysiotherapy with cycloferon can be a method of choice in the treatment of manifest HPV infection in males.

Study of red cell surface structure is a highly informative method for the assessment of the body status in health and disease. The authors have examined the surface red cell structure of infants in health and disease by phase-contrast and scanning electron microscopy and came to the conclusion on the high reliability of the data obtained by phase-contrast microscopy. They recommend this unsophisticated and informative method for clinical practice.

The energy expenditures in the nonproductive period in the women-workers of the boot and shoe industry (group I) and the sewing industry (group II) were almost similar: in group I it was on an average 1372 +/- 35.4 kcal, in group II--1384 +/- 27.6 kcal. In the productive period the energy expenditures in the workers of group I fluctuated from 1.56 to 2.09 kcal/min, in those of group II--from 1.78 to 2.27 kcal/min. Summary energy expenditures in the productive period comprised 877 +/- 91.1 kcal (in group I), and 949 +/- 51.1 kcal (in group II). Daily energy expenditures were 2249 +/- 77.1 kcal and 2333 +/- 63.9 kcal, respectively. The energy value of the rations of their actual nutrition insignificantly exceeded their energy expenditures (on an average by 120-150 kcal) due to excessive fat consumption, animal fat, in particular. Their rations were characterized by protein, and, to a lesser extent, carbohydrate deficiency, by imbalanced mineral composition and vitamin A, B1, B2, PP and C deficiency. Microsymptoms of vitamin deficiency (mainly those of vitamin C deficiency) were detected in 64% of the examined subjects, excessive weight was found in 23-26% and obesity in 11-16% of the women. The women working at the modern boot and shoe and sewing industry should be referred to the first category of the work intensity, with respect to the energy requirements and the energy value of the nutrient components of their food rations.

By using NGS-sequencing libraries of DNA from periodontal swabs with primers specific to V6 region of 16S rDNA prevalence of bacterial genera and species in periodontal microbiota of patients with aggressive periodontitis and healthy donors was analyzed. Six genera of putative periodontal protectors and eight periodontal pathogens were identified with respect to aggressive (but not chronic) periodontitis. Statistically relevant over-colonization by general Porphyromonas, Treponema, Synergistes, Tannerella, Filifactor, Ruminococcus, Parvimonas and Mycoplasma was found to be associated with the condition. From these, only three genera Porphyromonas, Treponema and Tannerella are traditionally considered as periodontal pathogens. Statistically confidential over-colonization by genus Veillonella was found in healthy patients. This genus should be considered as a relevant marker of a healthy periodontium. Genera Streptococcus, Bergeyella, Granulicatella, Kingella and Corynebacterium may be considered as putative periodontal protectors. Comparison of data of NGS-sequencing and real-time PCR demonstrated a good agreement if different PCR efficiency using independent primer pairs is taken into account.В работе с применением метода NGS-секвенирования банков суммарной ДНК пародонтальных смывов с праймерами на область V6 16S рДНК проанализирована представленность видов и родов бактерий в микрофлоре пародонта пациентов с агрессивным пародонтитом и лиц со здоровым пародонтом. В результате выявлено 6 родов потенциальных пародонтопротекторов и 8 родов потенциальных пародонтопатогенов, имеющих отношение к риску возникновения агрессивного (но не хронического) пародонтита. Выявлено статистически достоверное повышение представленности у больных агрессивным пародонтитом родов Porphyromonas, Treponema, Synergistes, Tannerella, Filifactor, Ruminococcus, Parvimonas, Mycoplasma, из которых только три Porphyromonas, Treponema и Tannerella традиционно рассматриваются как пародонтопатогены. У лиц со здоровым пародонтом было впервые выявлено статистически достоверное доминирование рода Veillonella, которое, таким образом, должно рассматриваться в качестве важного критерия здоровья пародонта. Роды Streptococcus, Bergeyella, Granulicatella, Kingella и Corynebacterium могут рассматриваться в качестве кандидатных пародонтопротекторов. Проведено сопоставление данных NGS-секвенирования и ПЦР в реальном времени. Показана хорошая воспроизводимость результатов при условии учета различной эффективности реакции ПЦР при использовании независимых пар праймеров.

By using qPCR system, women as well as men were found to have an equal periodontal pathogen colonization. However, the women are subjected to have a higher risk of chronic periodontitis onsets. Women with the chronic periodontitis usually expose an evident hypercolonization with a single pathogen. P. gingivalis is the most prevalent causative agent of the chronic periodontitis in women but not in men. In health as well as in the chronic periodontitis a complex of periodontal pathogens forms such as P. gingivalis, P. intermedia, T. forsythensis and T. denticola. T. forsythensis demonstrates the highest correlation with the chronic periodontitis onset in men. Our data allow us to prove T. forsythensis playing the key role in the forming of periodontal pathogen complex.С применением полимеразной цепной реакции в реальном времени установлено, что женщины, имея в среднем ту же степень обсемененности пародонта патогенами, что и мужчины, подвержены большему риску развития хронического генерализованного пародонтита (ХГП). При этом для женщин с ХГП характерно резкое доминирование какого-либо 1 пародонтопатогена: заболевание носит характер моноинфекции. Наиболее опасным возбудителем ХГП для женщин является Porphyromonas gingivalis. Для мужчин как в норме, так и при ХГП, характерно образование комплекса пародонтопатогенов в составе Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis и Treponema denticola. При этом у мужчин гиперколонизация пародонта T. forsythensis дает наиболее значимую ассоциацию с развитием ХГП. В совокупности результаты исследования позволяют предположить, что T. forsythensis играет ключевую роль в формировании комплекса пародонтопатогенов, вероятно, выступая инициатором этого процесса.