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2. The effects of ethidium bromide on the corticotrophin-induced transformation of mitochondria in rat adrenal cortical cells grown in tissue culture.

Author

Milner AJ

Subjects
Animals, Carbon Isotopes, Corticosterone biosynthesis, Culture Techniques, Desoxycorticosterone metabolism, Ethidium pharmacology, Fetus, Mitochondria metabolism, Progesterone metabolism, Rats, Adrenal Glands cytology, Adrenocorticotropic Hormone pharmacology, Cells, Cultured drug effects, Mitochondria drug effects, Phenanthridines pharmacology
Published
1972

3. Steroid biosynthesis by human foetal adrenals incubated in vitro with [4-14C) progesterone: changes with gestational age.

Author

Milner AJ and Mills IH

Subjects
17-Hydroxycorticosteroids biosynthesis, 17-Ketosteroids biosynthesis, Androstanes biosynthesis, Carbon Isotopes, Female, Fetus, Humans, Hydrocortisone biosynthesis, In Vitro Techniques, Ketones, Male, Organ Size, Pregnenolone metabolism, Radioisotope Dilution Technique, Adrenal Glands metabolism, Gestational Age, Progesterone metabolism, Steroids biosynthesis
Published
1970
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4. Patterns of steroid biosynthesis by human adrenals incubated in vitro with [7alpha-3H) pregnenolone: changes with (a) gestational age, (b) incubation period and (c) weight of incubated tissue.

Author

Milner AJ and Mills IH

Subjects
17-Hydroxycorticosteroids biosynthesis, 17-Ketosteroids biosynthesis, Adrenal Glands enzymology, Adult, Aged, Androstanes biosynthesis, Dehydroepiandrosterone biosynthesis, Female, Fetus, Humans, Hydrocortisone biosynthesis, Hydroxysteroid Dehydrogenases metabolism, In Vitro Techniques, Male, Middle Aged, Radioisotope Dilution Technique, Sulfates biosynthesis, Time Factors, Tritium, Adrenal Glands metabolism, Gestational Age, Pregnenolone metabolism, Steroids biosynthesis
Published
1970
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5. Cyclic AMP and the differentiation of adrenal cortical cells grown in tissue culture.

Author

Milner AJ

Subjects
Adrenal Glands drug effects, Adrenocorticotropic Hormone pharmacology, Animals, Carbon Isotopes, Corticosterone analysis, Culture Techniques, Desoxycorticosterone analysis, Female, Fetus, Microscopy, Electron, Mitochondria, Pregnancy, Progesterone biosynthesis, Rats, Adrenal Glands embryology, Cell Differentiation drug effects, Cyclic AMP pharmacology
Published
1972
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6. Steroidogenic and morphologic effects of ACTH on human fetal adrenal cells grown in tissue culture.

Author

Milner AJ and Villee DB

Subjects
Adrenal Glands metabolism, Aminocaproates biosynthesis, Carbon Isotopes, Cholesterol metabolism, Endoplasmic Reticulum drug effects, Fetus, Humans, Hydroxyprogesterones biosynthesis, Microscopy, Phase-Contrast, Mitochondria drug effects, Progesterone metabolism, Stimulation, Chemical, Transcortin metabolism, Adrenal Glands cytology, Adrenal Glands drug effects, Adrenocorticotropic Hormone pharmacology, Cholesterol pharmacology, Culture Techniques, Hydrocortisone biosynthesis, Progesterone pharmacology
Published
1970
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7. ACTH and the differentiation of rat adrenal cortical cells grown in primary tissue culture.

Author

Milner AJ

Subjects
Adrenal Glands embryology, Animals, Caproates biosynthesis, Carbon Isotopes, Cell Differentiation, Chloramphenicol pharmacology, Cholesterol metabolism, Chromatography, Thin Layer, Corticosterone biosynthesis, Desoxycorticosterone metabolism, Female, Fetus metabolism, Pregnancy, Progesterone metabolism, Rats, Adrenal Cortex Hormones biosynthesis, Adrenal Glands cytology, Adrenal Glands metabolism, Adrenocorticotropic Hormone pharmacology, Culture Techniques, Mitochondria metabolism
Published
1971
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8. Corticotrophin-induced differentiation of mitochondria in rat adrenal cortical cells grown in primary tissue culture: effects of ethidium bromide.

Author

Milner AJ

Subjects
Adrenal Glands enzymology, Animals, Culture Techniques, Fetus, Membranes enzymology, Mitochondria enzymology, Mixed Function Oxygenases antagonists & inhibitors, Rats, Adrenal Glands drug effects, Adrenocorticotropic Hormone antagonists & inhibitors, Cell Differentiation drug effects, Mitochondria drug effects, Quaternary Ammonium Compounds pharmacology, Quinolines pharmacology
Published
1972
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9. Morphological differentiation of adrenal cortical cells induced by a 40-minute exposure to corticotrophin.

Author

Milner AJ

Subjects
Adrenal Glands cytology, Adrenocorticotropic Hormone administration & dosage, Animals, Cell Differentiation drug effects, Culture Techniques, Endoplasmic Reticulum drug effects, Fetus, Microscopy, Electron, Mitochondria drug effects, Rats, Time Factors, Adrenal Glands drug effects, Adrenocorticotropic Hormone pharmacology
Published
1973
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11. Effects of human pituitary extracts on androgen biosynthesis by human adrenals in vitro.

Author

Milner AJ and Mills IH

Subjects
17-Ketosteroids biosynthesis, Adult, Aged, Androstanes biosynthesis, Chromatography, Paper, Chromatography, Thin Layer, Female, Fetus, Humans, Hydrocortisone biosynthesis, In Vitro Techniques, Male, Middle Aged, Pregnenolone metabolism, Progesterone metabolism, Adrenal Glands metabolism, Androgens biosynthesis, Pituitary Gland, Tissue Extracts pharmacology
Published
1970
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12. Ultrastructural criteria for assessing the functional integrity of endocrine cells in vitro.

Author

Milner AJ and Hamilton DW

Subjects
Adrenal Cortex Hormones biosynthesis, Adrenal Glands metabolism, Adrenocorticotropic Hormone pharmacology, Animals, Chloramphenicol pharmacology, Epididymis metabolism, In Vitro Techniques, Male, Microscopy, Electron, Organoids physiology, Rats, Testosterone biosynthesis, Vas Deferens metabolism, Adrenal Glands cytology, Adrenocorticotropic Hormone antagonists & inhibitors, Epididymis cytology, Vas Deferens cytology
Published
1971
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13. Patterns of steroid biosynthesis by human adrenals incubated in vitro with [7alpha-3H) pregnenolone: changes with (a) gestational age, (b) incubation period and (c) weight of incubated tissue

Author

Mills Ih and Milner Aj

Subjects
Adult, Male, medicine.medical_specialty, Radioisotope Dilution Technique, Time Factors, Hydrocortisone, Endocrinology, Diabetes and Metabolism, Gestational Age, Steroid biosynthesis, Biology, In Vitro Techniques, Tritium, Incubation period, Endocrinology, Fetus, Internal medicine, Adrenal Glands, medicine, Humans, Aged, 17-Hydroxycorticosteroids, Sulfates, Hydroxysteroid Dehydrogenases, Gestational age, Dehydroepiandrosterone, Middle Aged, In vitro, 17-Ketosteroids, Pregnenolone, Female, Steroids, Androstanes, medicine.drug
Abstract

SUMMARY Human adrenal tissue was sliced and incubated with 25 μc [7α-3H]-pregnenolone. The percentage conversion of radioactive precursor to dehydroepiandrosterone (DHA), DHA sulphate, androstenedione, 11β-hydroxyandrostenedione, cortisol and 11-deoxycortisol was estimated by the reverse isotope dilution technique. Incubations of both adult and foetal adrenal slices gave reproducible results. The duration of the incubation period was an important factor affecting the pattern of radioactive steroids synthesized, the adult adrenal pattern being dissimilar to that of the foetal adrenal. A direct proportional relationship between the weight of the incubated tissue and the biosynthesis of cortisol from exogenous [7α-3H]-pregnenolone was not established. The production of DHA sulphate relative to free DHA increased markedly from 12 to 27 weeks gestation. When incubated for 2 hr. the combined production of DHA and DHA sulphate relative to cortisol and androstenedione decreased with gestational age. There was evidence for considerable 3β-hydroxysteroid dehydrogenase activity in foetal adrenals at 12 to 27 weeks gestation.

Published
1970

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