1. Effect of Centaurium erythraea Rafn, Artemisia herba-alba Asso and Trigonella foenum-graecum L. on liver fat accumulation in C57BL/6J mice with high-fat diet-induced type 2 diabetes.
- Author
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Hamza, Nawel, Berke, Bénédicte, Cheze, Catherine, Marais, Sébastien, Lorrain, Simon, Abdouelfath, Abdelilah, Lassalle, Regis, Carles, Dominique, Gin, Henri, and Moore, Nicholas
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TYPE 2 diabetes prevention , *FATTY liver , *FATTY liver prevention , *HEPATOTOXICOLOGY , *LIVER analysis , *MEDICINAL plants , *ADIPOSE tissues , *ALTERNATIVE medicine , *ANIMAL experimentation , *BIOPHYSICS , *BODY weight , *FAT content of food , *HISTOLOGICAL techniques , *HYPOGLYCEMIC agents , *RESEARCH methodology , *MICE , *STAINS & staining (Microscopy) , *PLANT extracts , *DESCRIPTIVE statistics , *PHARMACODYNAMICS , *DIAGNOSIS , *PREVENTION - Abstract
Ethnopharmacological relevance Centaurium erythraea Rafn (CE), Artemisia herba-alba Asso (AHA) and Trigonella foenum-graecum L. (TFG) are traditionally used to treat type 2 diabetes in Algeria, previous studies have found that extracts of these plants were effective to treat or prevent experimental diabetes induced by high-fat diet (HFD). Aim of the study Describe the additional effects of these extracts on lipid tissue deposition in HFD. Materials and methods Male C57BL/6J mice were fed with HFD to induce type 2 Diabetes. Groups of mice were given plant extracts orally at 2 g/kg/bodyweight daily for 20 weeks during establishment of diabetes, or for 18 weeks after confirmation of diabetes at the 17th week. Liver and other tissue samples were stained with Oil Red O. Results Liver steatosis was confirmed with HFD. CE, AHA and TFG extracts improved liver steatosis by the end of the preventive (20 weeks) and curative periods (35 weeks). This was most marked for CE extract ( p <0.05), less so with TFG and AHA. No steatosis was found in other tissues. Conclusion CE extract had a clear hepatoprotective effect in this mouse model of diet-induced type 2 diabetes. AHA and TFG had a minimal or no significant effect on steatosis. Beyond its effect as an antidiabetic agent, CE may also be promising to prevent or treat non-alcoholic liver steatosis. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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