1. Mogrol Derived from Siraitia grosvenorii Mogrosides Suppresses 3T3-L1 Adipocyte Differentiation by Reducing cAMP-Response Element-Binding Protein Phosphorylation and Increasing AMP-Activated Protein Kinase Phosphorylation.
- Author
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Harada N, Ishihara M, Horiuchi H, Ito Y, Tabata H, Suzuki YA, Nakano Y, Yamaji R, and Inui H
- Subjects
- 3T3-L1 Cells, Adipocytes cytology, Animals, Cyclic AMP Response Element-Binding Protein metabolism, Lipid Metabolism, Mice, Phosphorylation, Signal Transduction, AMP-Activated Protein Kinases metabolism, Adipocytes drug effects, Cell Differentiation drug effects, Cucurbitaceae chemistry, Cyclic AMP Response Element-Binding Protein antagonists & inhibitors, Triterpenes pharmacology
- Abstract
This study investigated the effects of mogrol, an aglycone of mogrosides from Siraitia grosvenorii, on adipogenesis in 3T3-L1 preadipocytes. Mogrol, but not mogrosides, suppressed triglyceride accumulation by affecting early (days 0-2) and late (days 4-8), but not middle (days 2-4), differentiation stages. At the late stage, mogrol increased AMP-activated protein kinase (AMPK) phosphorylation and reduced glycerol-3-phosphate dehydrogenase activity. At the early stage, mogrol promoted AMPK phosphorylation, inhibited the induction of CCAAT/enhancer-binding protein β (C/EBPβ; a master regulator of adipogenesis), and reduced 3T3-L1 cell contents (e.g., clonal expansion). In addition, mogrol, but not the AMPK activator AICAR, suppressed the phosphorylation and activity of the cAMP response element-binding protein (CREB), which regulates C/EBPβ expression. These results indicated that mogrol suppressed adipogenesis by reducing CREB activation in the initial stage of cell differentiation and by activating AMPK signaling in both the early and late stages of this process., Competing Interests: The commercial affiliation along with any other relevant declarations relating to employment, consultancy, patents, products in development, or marketed products does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.
- Published
- 2016
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