1. The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites
- Author
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Camilla Bean, Matteo Audano, Tatiana Varanita, Francesca Favaretto, Marta Medaglia, Marco Gerdol, Lena Pernas, Fabio Stasi, Marta Giacomello, Stèphanie Herkenne, Maheswary Muniandy, Sini Heinonen, Emma Cazaly, Miina Ollikainen, Gabriella Milan, Alberto Pallavicini, Kirsi H. Pietiläinen, Roberto Vettor, Nico Mitro, Luca Scorrano, Bean, C., Audano, M., Varanita, T., Favaretto, F., Medaglia, M., Gerdol, M., Pernas, L., Stasi, F., Giacomello, M., Herkenne, S., Muniandy, M., Heinonen, S., Cazaly, E., Ollikainen, M., Milan, G., Pallavicini, A., Pietilainen, K. H., Vettor, R., Mitro, N., and Scorrano, L.
- Subjects
Jumonji Domain-Containing Histone Demethylases ,Endocrinology, Diabetes and Metabolism ,Adipocytes, White ,Mice, Transgenic ,White ,Diet, High-Fat ,Transgenic ,GTP Phosphohydrolases ,GTP Phosphohydrolase ,Mitochondrial Proteins ,Mice ,Physiology (medical) ,Internal Medicine ,Adipocytes ,Mitochondrial Protein ,Animals ,Humans ,Urea ,Adipocytes, Beige ,Obesity ,Cyclic AMP Response Element-Binding Protein ,Uncoupling Protein 1 ,Adipocytes, Brown ,Adipose Tissue ,Gene Expression Regulation ,Mitochondria ,Thermogenesis ,Metabolic Networks and Pathways ,Animal ,Beige ,Thermogenesi ,Jumonji Domain-Containing Histone Demethylase ,Brown ,Cell Biology ,Diet ,High-Fat ,Human - Abstract
White to brown/beige adipocytes conversion is a possible therapeutic strategy to tackle the current obesity epidemics. While mitochondria are key for energy dissipation in brown fat, it is unknown if they can drive adipocyte browning. Here, we show that the mitochondrial cristae biogenesis protein optic atrophy 1 (Opa1) facilitates cell-autonomous adipocyte browning. In two cohorts of patients with obesity, including weight discordant monozygotic twin pairs, adipose tissue OPA1 levels are reduced. In the mouse, Opa1 overexpression favours white adipose tissue expandability as well as browning, ultimately improving glucose tolerance and insulin sensitivity. Transcriptomics and metabolomics analyses identify the Jumanji family chromatin remodelling protein Kdm3a and urea cycle metabolites, including fumarate, as effectors of Opa1-dependent browning. Mechanistically, the higher cyclic adenosine monophosphate (cAMP) levels in Opa1 pre-adipocytes activate cAMP-responsive element binding protein (CREB), which transcribes urea cycle enzymes. Flux analyses in pre-adipocytes indicate that Opa1-dependent fumarate accumulation depends on the urea cycle. Conversely, adipocyte-specific Opa1 deletion curtails urea cycle and beige differentiation of pre-adipocytes, and is rescued by fumarate supplementation. Thus, the urea cycle links the mitochondrial dynamics protein Opa1 to white adipocyte browning.
- Published
- 2021