1. Recombinant adenovirus-delivered soluble CD163 and sialoadhesin receptors protected pigs from porcine reproductive and respiratory syndrome virus infection.
- Author
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Xia W, Wu Z, Guo C, Zhu S, Zhang X, Xia X, and Sun H
- Subjects
- Animals, Antibodies, Viral, Antigens, CD administration & dosage, Antigens, CD genetics, Antigens, Differentiation, Myelomonocytic administration & dosage, Antigens, Differentiation, Myelomonocytic genetics, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Genetic Vectors, Lung immunology, Lung pathology, Lung virology, Macrophages, Alveolar virology, Porcine Reproductive and Respiratory Syndrome immunology, Porcine Reproductive and Respiratory Syndrome virology, Porcine respiratory and reproductive syndrome virus pathogenicity, Receptors, Cell Surface administration & dosage, Receptors, Cell Surface genetics, Sialic Acid Binding Ig-like Lectin 1 administration & dosage, Sialic Acid Binding Ig-like Lectin 1 genetics, Swine, Viral Load, Viremia, Adenoviridae genetics, Antigens, CD immunology, Antigens, Differentiation, Myelomonocytic immunology, Porcine Reproductive and Respiratory Syndrome prevention & control, Porcine respiratory and reproductive syndrome virus immunology, Receptors, Cell Surface immunology, Sialic Acid Binding Ig-like Lectin 1 immunology
- Abstract
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases affecting pig industry worldwide. Sialoadehesin (Sn) and CD163 are the two specific receptors for PRRSV infection of porcine alveolar macrophages. Our previous study showed that the soluble Sn receptor Sn4D-Fc and soluble CD163 receptor SRCR59-Fc expressed by the two recombinant adenoviral (rAd) vectors have an additive anti-PRRSV effect in vitro. In the present study, rAd-Sn4D-Fc and rAd-SRCR59-Fc were inoculated into pigs, and the efficient expression of Sn4D-Fc and SRCR59-Fc proteins was detected by ELISA. Then, PRRSV-naïve pigs were inoculated with rAd-Sn4D-Fc and/or rAd-SRCR59-Fc before contagious infection with different PRRSV strains. Among the three rAd inoculation groups, simultaneous inoculation with the two rAd vectors provided the best protection against highly pathogenic JXA1 strain PRRSV, followed by rAd-SRCR59-Fc inoculation and rAd-Sn4D-Fc inoculation. Clinical observation and quantitative RT-PCR analyses showed that all of the double rAd-inoculated pigs (n = 9) survived from the contagious infection with highly pathogenic JXA1, JS07 or SH1705 strain PRRSV with significantly alleviated clinical scores, viremia, fecal viral emission and tissue virus loads. These data suggest that rAd-Sn4D-Fc and rAd-SRCR59-Fc can be developed further as the universal therapeutic vaccine to facilitate PRRSV eradication., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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