1. Sublingual administration of a helper-dependent adenoviral vector expressing the codon-optimized soluble fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in mice.
- Author
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Fu YH, Jiao YY, He JS, Giang GY, Zhang W, Yan YF, Ma Y, Hua Y, Zhang Y, Peng XL, Shi CX, and Hong T
- Subjects
- Administration, Sublingual, Animals, Antibodies, Neutralizing blood, Antibodies, Viral blood, Disease Models, Animal, Female, Immunity, Mucosal, Immunoglobulin G blood, Mice, Inbred BALB C, Respiratory Syncytial Virus Infections immunology, Respiratory Syncytial Virus Vaccines genetics, Respiratory Syncytial Virus, Human genetics, Serum immunology, Vaccines, Synthetic administration & dosage, Adenoviridae genetics, Drug Carriers administration & dosage, Respiratory Syncytial Virus Infections prevention & control, Respiratory Syncytial Virus Vaccines administration & dosage, Respiratory Syncytial Virus Vaccines immunology, Respiratory Syncytial Virus, Human immunology
- Abstract
Sublingual (s.l.) immunization has been described as a convenient and safe way to induce mucosal immune responses in the respiratory and genital tracts. We constructed a helper-dependent adenoviral (HDAd) vector expressing a condon-optimized soluble fusion glycoprotein (sFsyn) of respiratory syncytial virus (HDAd-sFsyn) and explored the potential of s.l. immunization with HDAd-sFsyn to stimulate immune responses in the respiratory mucosa. The RSV specific systemic and mucosal immune responses were generated in BALB/c mice, and the serum IgG with neutralizing activity was significantly elevated after homologous boost with s.l. application of HDAd-sFsyn. Humoral immune responses could be measured even 14weeks after a single immunization. Upon challenge, s.l. immunization with HDAd-sFsyn displayed an effective protection against RSV infection. These findings suggest that s.l. administration of HDAd-sFsyn acts as an effective and safe mucosal vaccine against RSV infection, and may be a useful tool in the prevention of RSV infection., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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