1. [Construction and confirmation of a recombinant adenovirus vector of survivin].
- Author
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Lü YJ, Miao SS, Liu WS, He HJ, Jia SS, He GQ, and Xiang C
- Subjects
- HEK293 Cells, Humans, Inhibitor of Apoptosis Proteins metabolism, Plasmids, Polymerase Chain Reaction, Recombinant Fusion Proteins metabolism, Survivin, Transfection, Adenoviridae genetics, Genetic Vectors, Inhibitor of Apoptosis Proteins genetics, Recombinant Fusion Proteins genetics
- Abstract
Objective: To construct a recombinant adenovirus of survivin vector and provid valuable reference for gene therapy of laryngeal cancer., Methods: The survivin gene was cloned by PCR. After confirmation by enzyme restriction analysis and sequencing, the gene and the adenovirus vector were recombined together to construct the recombinant adenovirus vector. The recombinant adenovirus vector was confirmed via both sequencing and digestion restriction analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenovirus., Results: The sequence analysis demonstrated that the survivin gene sequence was the same as published in the literature, suggesting that a recombinant adenovirus vector has been successfully constructed., Conclusions: A survivin recombinant adenovirus has been successfully constructed.
- Published
- 2011