1. Prognostic value of mucin 4 expression in colorectal adenocarcinomas.
- Author
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Shanmugam C, Jhala NC, Katkoori VR, Wan W, Meleth S, Grizzle WE, and Manne U
- Subjects
- Adenocarcinoma metabolism, Adenocarcinoma mortality, Adenocarcinoma pathology, Aged, Colorectal Neoplasms metabolism, Colorectal Neoplasms mortality, Colorectal Neoplasms pathology, Female, Humans, Male, Mucin-4, Prognosis, Adenocarcinoma diagnosis, Colorectal Neoplasms diagnosis
- Abstract
Background: Mucin 4 (MUC4) is aberrantly expressed in colorectal adenocarcinomas (CRCs) but its prognostic value is unknown., Methods: Archival tissue specimens collected from 132 CRC patients who underwent surgical resection without presurgery or postsurgery therapy were evaluated for expression of MUC4 by using a mouse monoclonal antibody and horseradish peroxidase. MUC4 expression levels were correlated with clinicopathologic features and patient survival. Survival was estimated by both univariate Kaplan-Meier and multivariate Cox regression methods., Results: In both normal colonic epithelium and CRCs, MUC4 staining was localized primarily in the cytoplasm. The optimal immunostaining cutoff value (>or=75% positive cells and an immunostaining score>or=2.0), which was derived by using the bootstrap method, was used to categorize CRCs into groups of high expression (33 of 132 patients; 25%) or low expression (99 of 132 patients; 75%). Patients who had early stage tumors (stages I and II) with high MUC4 expression had a shorter disease-specific survival (log-rank; P=.007) than patients who had with low expression. Patients who had advanced-stage CRCs (stages III and IV) did not demonstrate such a difference (log-rank; P=.108). Multivariate regression models that were generated separately for patients with early stage and advanced-stage CRC confirmed that increased expression of MUC4 was an independent indicator of a poor prognosis only for patients who had early stage CRCs (hazard ratio, 3.77; 95% confidence interval, 1.46-9.73)., Conclusions: The current results indicated that increased MUC4 expression is a predictor of poor survival in CRC, specifically for patients who have early stage tumors., (Copyright (c) 2010 American Cancer Society.)
- Published
- 2010
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