1. Identification of a STOP1-like protein in Eucalyptus that regulates transcription of Al tolerance genes.
- Author
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Sawaki Y, Kobayashi Y, Kihara-Doi T, Nishikubo N, Kawazu T, Kobayashi M, Kobayashi Y, Iuchi S, Koyama H, and Sato S
- Subjects
- Adaptation, Physiological drug effects, Agrobacterium metabolism, Amino Acid Sequence, Arabidopsis drug effects, Arabidopsis genetics, Arabidopsis physiology, Citrates metabolism, Eucalyptus drug effects, Gene Expression Regulation, Plant, Genetic Complementation Test, Genetic Vectors metabolism, Green Fluorescent Proteins metabolism, Molecular Sequence Data, Mutation, Phylogeny, Plant Proteins chemistry, Plant Proteins genetics, Plant Roots drug effects, Plant Roots growth & development, Plants, Genetically Modified, RNA Interference drug effects, RNA, Messenger genetics, RNA, Messenger metabolism, Sequence Alignment, Sequence Homology, Nucleic Acid, Adaptation, Physiological genetics, Aluminum toxicity, Eucalyptus genetics, Eucalyptus physiology, Genes, Plant, Plant Proteins metabolism, Transcription, Genetic drug effects
- Abstract
Tolerance to soil acidity is an important trait for eucalyptus clones that are introduced to commercial forestry plantations in pacific Asian countries, where acidic soil is dominant in many locations. A conserved transcription factor regulating aluminum (Al) and proton (H⁺) tolerance in land-plant species, STOP1 (SENSITIVE TOPROTON RHIZOTOXICITY 1)-like protein, was isolated by polymerase chain reaction-based cloning, and then suppressed by RNA interference in hairy roots produced by Agrobacterium rhizogenes-mediated transformation. Eucalyptus STOP1-like protein complemented proton tolerance in an Arabidopsis thaliana stop1-mutant, and localized to the nucleus in a transient assay of a green fluorescent protein fusion protein expressed in tobacco leaves by Agrobacterium tumefaciens-mediated transformation. Genes encoding a citrate transporting MULTIDRUGS AND TOXIC COMPOUND EXTRUSION protein and an orthologue of ALUMINUM SENSITIVE 3 were suppressed in transgenic hairy roots in which the STOP1 orthologue was knocked down. In summary, we identified a series of genes for Al-tolerance in eucalyptus, including a gene for STOP1-like protein and the Al-tolerance genes it regulates. These genes may be useful for molecular breeding and genomic selection of elite clones to introduce into acid soil regions., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2014
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