1. High incorporation of long-chain fatty acids contributes to the efficient production of acylated ghrelin in ghrelin-producing cells.
- Author
-
Bando M, Iwakura H, Koyama H, Hosoda H, Shigematsu Y, Ariyasu H, Akamizu T, Kangawa K, and Nakao K
- Subjects
- Acylation, Acyltransferases antagonists & inhibitors, Acyltransferases genetics, Animals, Carnitine analogs & derivatives, Carnitine metabolism, Cell Line, Coenzyme A Ligases antagonists & inhibitors, Coenzyme A Ligases genetics, Enteroendocrine Cells cytology, Enteroendocrine Cells drug effects, Enzyme Inhibitors pharmacology, Fatty Acids, Nonesterified chemistry, Gene Expression Profiling, Gene Expression Regulation drug effects, Insulin-Secreting Cells cytology, Insulin-Secreting Cells drug effects, Insulin-Secreting Cells metabolism, Lipoylation, Membrane Proteins, Mice, Transgenic, Molecular Weight, RNA Interference, Recombinant Fusion Proteins, Triazenes pharmacology, Acyltransferases metabolism, Coenzyme A Ligases metabolism, Enteroendocrine Cells metabolism, Fatty Acids, Nonesterified metabolism, Ghrelin metabolism, Protein Processing, Post-Translational drug effects
- Abstract
The mechanisms for supplying octanoic acid for ghrelin acylation in X/A-like cells are incompletely understood. We found that long-chain fatty acids were incorporated at a higher rate in the ghrelin-producing cell line MGN3-1 than in MIN6 cells, in part due to higher expression level of long-chain fatty acyl-CoA synthetase family member 1 (Acsl1). Inhibition of ACSLs by triacsin C profoundly suppressed acylated ghrelin production. These results suggest that high incorporation of long-chain fatty acids into the ghrelin-producing cells plays a role in the supply of octanoic acid for ghrelin acylation., (© 2016 Federation of European Biochemical Societies.)
- Published
- 2016
- Full Text
- View/download PDF