1. MMP protein and activity levels in synovial fluid from patients with joint injury, inflammatory arthritis, and osteoarthritis
- Author
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Johan M. TeKoppele, I Tchetverikov, Nicole Verzijl, Roeland Hanemaaijer, Jeroen DeGroot, L S Lohmander, T.W.J. Huizinga, and TNO Preventie en Gezondheid
- Subjects
Male ,Pathology ,Biomedical Research ,Inflammatory arthritis ,Arthritis ,knee ,ComputingMilieux_LEGALASPECTSOFCOMPUTING ,Osteoarthritis ,synovial fluid level ,pseudogout ,Synovial Fluid ,Immunology and Allergy ,enzyme inhibition ,tissue inhibitor of metalloproteinase 3 ,alpha 2 macroglobulin ,quantitative analysis ,Middle Aged ,Osteoarthritis, Knee ,Macroglobulin ,unclassified drug ,enzyme activity ,Extended Report ,priority journal ,Acute Disease ,Female ,interstitial collagenase ,musculoskeletal diseases ,Adult ,medicine.medical_specialty ,matrix metalloproteinase ,Immunology ,Chondrocalcinosis ,Knee Injuries ,General Biochemistry, Genetics and Molecular Biology ,Rheumatology ,prostromelysin ,medicine ,Synovial fluid ,joint injury ,Humans ,controlled study ,alpha-Macroglobulins ,human ,immunoassay ,Rheumatology and Autoimmunity ,Tissue Inhibitor of Metalloproteinase-1 ,business.industry ,enzyme activation ,Tissue inhibitor of metalloproteinase ,medicine.disease ,stromelysin ,major clinical study ,Matrix Metalloproteinases ,osteoarthritis ,Cross-Sectional Studies ,prointerstitial collagenase ,Pseudogout ,business ,protein determination - Abstract
Objective: To determine protein and activity levels of matrix metalloproteinases 1 and 3 (MMP-1 and MMP-3) in synovial fluid of patients with knee joint injury, primary osteoarthritis, and acute pyrophosphate arthritis (pseudogout). Methods: Measurements were done on knee synovial fluid obtained in a cross sectional study of cases of injury (n=283), osteoarthritis (n=105), and pseudogout (n=65), and in healthy controls (n=35). Activity of MMP-1 and MMP-3 in α2 macroglobulin complexes was measured using specific low molecular weight fluorogenic substrates. ProMMP-1, proMMP-3, and TIMP-1 (tissue inhibitor of metalloproteinase 1) were quantified by immunoassay. Results: Mean levels of proMMP-1, proMMP-3, and TIMP-1 were increased in injury, osteoarthritis, and pseudogout compared with controls. MMP-1 activity was increased in pseudogout and injury groups over control levels, whereas MMP-3 activity was increased only in the pseudogout group. The increase in MMP-1 activity coincided with a decrease in TIMP-1 levels in the injury group. Conclusions: Patients with joint injury have a persistent increase in proMMP-1 and proMMP-3 in synovial fluid and an increase in activated MMPs, which are not inhibited by TIMP. The differences in activation and inhibition patterns between the study groups are consistent with disease specific patterns of MMP activation and/or inhibition in joint pathology. Chemicals / CAS: alpha 2 macroglobulin, 95568-41-5; interstitial collagenase, 9001-12-1; stromelysin, 79955-99-0; tissue inhibitor of metalloproteinase 3, 145809-21-8, 164781-40-2; alpha-Macroglobulins; Matrix Metalloproteinases, EC 3.4.24.-; Tissue Inhibitor of Metalloproteinase-1
- Published
- 2005