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Your search keyword '"M. T. Dobrynina"' showing total 3 results
Start Over Author "M. T. Dobrynina" Topic acute colitis
3 results on '"M. T. Dobrynina"'

1. Sex differences of subpopulation composition of lymphocytes in the peripheral blood in experimental acute and chronic ulcerative colitis

Author

O. V. Makarova, E. A. Postovalova, Yu. Gao, and M. T. Dobrynina

Subjects
sex differences, acute colitis, chronic colitis, c57bl/6 mice, lymphocytes subpopulations, Immunologic diseases. Allergy, RC581-607
Abstract

We studied sex differences lymphocytes subpopulations of peripheral blood in adult C57Bl/6 mice during acute and chronic colitis, induced with 1% DSS. We measured subpopulations of lymphocytes with flow cytometry. We showed that in the control group the female mice had statistically significantly higher values of the relative number of regulatory and cytotoxic T lymphocytes comparing to the males. During acute colitis the females showed an increase in the relative number of Thelpers and a decrease of cytotoxic Tlymphocytes, which reflects the activation of immune response. The males had a decrease in the absolute number of leukocytes, lymphocytes and cytotoxic and regulatory T lymphocytes, probably because of an increase in migration of these cells to the inflammation locus and local lymph nodes. In chronic colitis the females had a decrease in the absolute number of leukocytes, lymphocytes, T helpers, cytotoxic T lymphocytes and B lymphocytes when comparing with acute colitis. During chronic colitis the males had a decrease in the absolute number of T helpers and B lymphocytes but an increase of regulatory T cells in comparison with the control group; in comparison with acute colitis the males with chronic colitis had higher relative and absolute number of regulatory T cells. The increase of T regulatory lymphocytes is due to an increase in their proliferation rate in the thymus and increase of their migration to the inflammatory locus – the colon. Future clinical studies may be based on these results, which show that the treatment of colitis, especially with immunotropic agents, must take sex differences into account.

Published
2020
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2. Structural Changes in the Nervous Fibers of the Colon Mucosa in Experimental Acute Colitis

Author

V A Mkhitarov, V P Chernikov, M T Dobrynina, L P Mikhailova, O V Makarova, A. S. Sladkopevtsev, and D N Khochanskiy

Subjects
Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Colon, General Biochemistry, Genetics and Molecular Biology, Muscle hypertrophy, Mice, 03 medical and health sciences, chemistry.chemical_compound, Nerve Fibers, 0302 clinical medicine, medicine, Submucous plexus, Animals, Intestinal Mucosa, Colitis, Acute colitis, business.industry, General Medicine, Hyperplasia, medicine.disease, Ulcerative colitis, Mice, Inbred C57BL, Disease Models, Animal, Microscopy, Electron, 030104 developmental biology, Dextran, chemistry, Acute Disease, Ultrastructure, Colitis, Ulcerative, business, 030217 neurology & neurosurgery
Abstract

Morphological, morphometric, and ultrastructural analysis of the nerve fibers in the colon mucosa was performed in C57BL/6 mice at various terms of development of acute colitis induced by dextran sodium sulphate. The nerve fibers were labeled with antibodies to pan-neuronal marker βIII-tubulin. The progression of inflammatory and ulcerative processes in the mucosa on days 3-5 was associated with hyperplasia and hypertrophy of nerve fibers that peaked on day 7 after colitis induction. Ultrastructural analysis at all terms of colitis development showed moderate degeneration of axons. Thus, hypertrophy and hyperplasia of the nervous fibers in colon mucosa in experimental acute colitis correlated with aggravation of the ulcerative process in the mucosa. These changes are determined by alteration of histoarchitectonics and regenerative processes in the mucosa.

Published
2020

3. Sex-Related Differences in the Morphology and Subpopulation Composition of Colon Lymphocytes in Experimental Acute Colitis

Author

Yu Gao, E A Postovalova, O V Makarova, L P Mikhailova, and M T Dobrynina

Subjects
0301 basic medicine, Male, medicine.medical_specialty, Colon, CD3, Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Mice, Sex Factors, Internal medicine, medicine, Cytotoxic T cell, Animals, IL-2 receptor, Lymphocytes, Colitis, Acute colitis, chemistry.chemical_classification, FOXP3, General Medicine, medicine.disease, Lymphocyte Subsets, Mice, Inbred C57BL, Disease Models, Animal, 030104 developmental biology, Endocrinology, Enzyme, chemistry, biology.protein, Colitis, Ulcerative, Female, CD8
Abstract

Morphological manifestations of acute colitis and subpopulation composition of colon lymphocytes were studied in male and female C57Bl/6 mice with acute dextran-induced colitis. We evaluated the severity of colitis symptoms, morphological changes in the colon, and prevalence of epithelialized and non-epithelialized ulcers. The subpopulation composition of lymphocytes (CD3-CD19+ B cells, CD3+CD4+ T helpers, CD3+CD8+ cytotoxic T cells, and CD4+CD25+FOXP3+ regulatory T cells) was assessed by flow cytofluorometry in suspension of colon cells prepared by enzymatic disintegration. In males, clinical manifestations of acute colitis and morphological changes were more severe and the prevalence of non-epithelialized ulcers was higher than in females. In females, the content of T, B, and regulatory T cells in the colon wall was higher, while the content of cytotoxic T cells was lower than in males. In females with acute colitis, the absolute lymphocyte count and the content of B cells and regulatory T cells decreased, while the percentage of cytotoxic T cells increased in comparison with intact animals. In males with acute colitis, the levels of regulatory T and B cells increased in comparison with the corresponding parameter in intact animals. Morphological changes and changes in the lymphocyte subpopulations, detected in males and females with acute colitis, were determined by different levels of sex steroid hormones.

Published
2018

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