1. Intertwined control of the cell cycle and nucleocytoplasmic transport by the cyclin-dependent kinase Pho85 and RanGTPase Gsp1 in Saccharomyces cerevisiae.
- Author
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Mirallas O, Ballega E, Samper-Martín B, García-Márquez S, Carballar R, Ricco N, Jiménez J, and Clotet J
- Subjects
- Base Sequence, Cyclin-Dependent Kinases genetics, Escherichia coli genetics, Homologous Recombination, Monomeric GTP-Binding Proteins genetics, Mutagenesis, Site-Directed, Nuclear Proteins genetics, Protein Binding, Recombinant Proteins, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Active Transport, Cell Nucleus physiology, Cell Cycle physiology, Cyclin-Dependent Kinases metabolism, Monomeric GTP-Binding Proteins metabolism, Nuclear Proteins metabolism, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Deciphering the molecular mechanisms that connect cell cycle progression and nucleocytoplasmic transport is of particular interest: this intertwined relationship, once understood, may provide useful insight on the diseases resulting from the malfunction of these processes. In the present study we report on findings that indicate a biochemical connection between the cell cycle regulator CDK Pho85 and Ran-GTPase Gsp1, an essential nucleocytoplasmic transport component. When Gsp1 cannot be phosphorylated by Pho85, the cell cycle progression is impaired. Accordingly, a nonphosphorylatable version of Gsp1 abnormally localizes to the nucleus, which impairs the nuclear transport of molecules, including key components of cell cycle progression. Furthermore, our results suggest that the physical interaction of Gsp1 and the Kap95 karyopherin, essential to the release of nuclear cargoes, is altered. Altogether, the present findings point to the involvement of a biochemical mechanism in the interlocked regulation of the cell cycle and nuclear transport., (Copyright © 2017 Elsevier GmbH. All rights reserved.)
- Published
- 2018
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