1. G-actin and F-actin levels at different stages of mouse erythroid differentiation
- Author
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Samuel Waxman, William Scher, Barbara M. Scher, and Helena S. Yeh
- Subjects
Erythrocytes ,Reticulocytes ,Microgram ,macromolecular substances ,Cell Biology ,Biology ,medicine.disease ,Molecular biology ,Actins ,Cell Line ,Mice ,Leukemia ,Cell culture ,medicine ,Animals ,Erythropoiesis ,Dimethyl Sulfoxide ,Leukemia, Erythroblastic, Acute ,Actin - Abstract
Monomeric (G-) actin and filamentous (F-) actin levels were determined in Triton X-100 extracts prepared from mouse erythroid cells at various stages of differentiation. G-actin and F-actin were found in the Triton-soluble fraction and in the Triton-insoluble fraction, respectively. G-actin levels in untreated and dimethyl sulfoxide-treated (differentiated) erythroleukemia cells, reticulocytes, and erythrocytes were 48, 33, 2.8, and 0.37 microgram/mg protein, respectively, and F-actin levels were 17, 35, 45, and 59 micrograms/mg protein, respectively. G-actin/F-actin ratios were successively lower in cells representing the more mature stages of development.
- Published
- 1983
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