1. Allonocardiopsis opalescens gen. nov., sp. nov., a new member of the suborder Streptosporangineae, from the surface-sterilized fruit of a medicinal plant.
- Author
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Du HJ, Zhang YQ, Liu HY, Su J, Wei YZ, Ma BP, Guo BL, and Yu LY
- Subjects
- Actinomycetales genetics, Actinomycetales isolation & purification, Bacterial Typing Techniques, China, DNA, Bacterial genetics, Fatty Acids analysis, Molecular Sequence Data, Plants, Medicinal microbiology, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Vitamin K 2 analysis, Actinomycetales classification, Fruit microbiology, Lonicera microbiology, Phylogeny
- Abstract
An endophytic actinomycete, designated strain I10A-01259(T), was isolated from a surface-sterilized fruit of Lonicera maackii (Rupr.) Maxim., a medicinal plant, which was collected from a suburb of Beijing, China. Whole-cell hydrolysates of the isolate contained galactose and meso-diaminopimelic acid. The predominant phospholipids were phosphatidylglycerol and diphosphatidylglycerol; the menaquinones consisted mainly of MK-9, MK-11 and MK-12, with a minor amount of MK-10. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. Comparative analysis of 16S rRNA gene sequences indicated that strain I10A-01259(T) was most closely related to Nocardiopsis arabia S186(T) (93.2 % sequence similarity), Thermobifida halotolerans YIM 90462(T) (93.0 %) and other strains of genera within the families Nocardiopsaceae and Thermomonosporaceae. On the phylogenetic tree based on 16S rRNA gene sequences, strain I10A-01259(T) fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to the genera of the families Nocardiopsaceae and Thermomonosporaceae. Based on the data from our polyphasic taxonomic study, a novel genus and species, Allonocardiopsis opalescens gen. nov., sp. nov., are proposed within the suborder Streptosporangineae. The type strain of Allonocardiopsis opalescens is strain I10A-01259(T) ( = CPCC 203428(T) = DSM 45601(T) = KCTC 19844(T)).
- Published
- 2013
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