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Environmental and ecological factors can trigger changes in the acoustic repertoire of cetaceans. This study documents the first use of a well-established passive acoustic monitoring device (C-POD) to analyze echolocation sounds and behavior of franciscana dolphins in different habitats: estuary [Babitonga Bay (BB)] and open sea [Itapiruba Beach (IB)]. A total of 10 924 click trains were recorded in BB and 6 093 in IB. An inter-click interval

Anthropogenic noise in the world's oceans is known to impede many species' ability to perceive acoustic signals, but little research has addressed how this noise affects the perception of bioacoustic signals used for communication in marine mammals. Bottlenose dolphins (Tursiops truncatus) use signature whistles containing identification information. Past studies have used human participants to gain insight into dolphin perception, but most previous research investigated echolocation. In Experiment 1, human participants were tested on their ability to discriminate among signature whistles from three dolphins. Participants' performance was nearly errorless. In Experiment 2, participants identified signature whistles masked by five different samples of boat noise utilizing different signal-to-noise ratios. Lower signal-to-noise ratio and proximity in frequency between the whistle and noise both significantly decreased performance. Like dolphins, human participants primarily identified whistles using frequency contour. Participants reported greater use of amplitude in noise-present vs noise-absent trials, but otherwise did not vary cue usage. These findings can be used to generate hypotheses about dolphins' performance and auditory cue use for future research. This study may provide insight into how specific characteristics of boat noise affect dolphin whistle perception and may have implications for conservation and regulations.

This paper investigates the evolution of spectral properties observed in Cuvier's beaked whale (Ziphius cavirostris) click trains recorded by fixed hydrophones in the Gulf of Mexico. In the context of deep water and high-frequency sounds and observed inter-click intervals, the authors assumed that the main effect responsible for the modification of the spectral content between adjacent clicks in the same click train is the source beam pattern. The spectral structure is studied by using the Wigner-Ville time-frequency distribution and is compared with the conventional Fourier spectrogram. The results show that the observed Cuvier's beaked whale clicks are a superposition of upsweep and downsweep chirps, unlike the currently accepted upsweep only structure of beaked whale clicks in bioacoustics literature. The spectral structure variations simulated by using a flat circular piston model as a beam pattern transmission model are consistent with the evolution of spectral click properties observed in experimental data. A better understanding of the properties of observed echolocation clicks of Cuvier's beaked whales will provide useful information for click annotations and, therefore, will contribute to improving accuracy of detecting, classifying, tracking, and estimating the density of Cuvier's beaked whales.

Sablefish sounds, named rasps, were recorded at two captive facilities in British Columbia and Washington State. Rasps consisted of highly variable broadband trains of 2 to 336 ticks that lasted between 74 and 10 500 ms. The 260 rasps that were measured contained frequencies between 344 and 34 000 Hz with an average peak frequency of 3409 Hz. The frequency structure of ticks within rasps was highly variable and included both positive and negative trends. This finding makes sablefish one of the few deep-sea fish for which sounds have been validated and described. The documentation of sablefish sounds will enable the use of passive acoustic monitoring methods in fisheries and ecological studies of this commercially important deep-sea fish.

Source separation is an important step to study signals that are not easy or possible to record individually. Common methods such as deep clustering, however, cannot be applied to signals of an unknown number of sources and/or signals that overlap in time and/or frequency-a common problem in bioacoustic recordings. This work presents an approach, using a supervised learning framework, to parse individual sources from a spectrogram of a mixture that contains a variable number of overlapping sources. This method isolates individual sources in the time-frequency domain using only one function but in two separate steps, one for the detection of the number of sources and corresponding bounding boxes, and a second step for the segmentation in which masks of individual sounds are extracted. This approach handles the full separation of overlapping sources in both time and frequency using deep neural networks in an applicable manner to other tasks such as bird audio detection. This paper presents method and reports on its performance to parse individual bat signals from recordings containing hundreds of overlapping bat echolocation signals. This method can be extended to other bioacoustic recordings with a variable number of sources and signals that overlap in time and/or frequency.

Classical ocean acoustic experiments involve the use of synchronized arrays of sensors. However, the need to cover large areas and/or the use of small robotic platforms has evoked interest in single-hydrophone processing methods for localizing a source or characterizing the propagation environment. One such processing method is "warping," a non-linear, physics-based signal processing tool dedicated to decomposing multipath features of low-frequency transient signals (frequency f 500 Hz), after their propagation through shallow water (depth D 200 m) and their reception on a distant single hydrophone (range r 1 km). Since its introduction to the underwater acoustics community in 2010, warping has been adopted in the ocean acoustics literature, mostly as a pre-processing method for single receiver geoacoustic inversion. Warping also has potential applications in other specialties, including bioacoustics; however, the technique can be daunting to many potential users unfamiliar with its intricacies. Consequently, this tutorial article covers basic warping theory, presents simulation examples, and provides practical experimental strategies. Accompanying supplementary material provides matlab code and simulated and experimental datasets for easy implementation of warping on both impulsive and frequency-modulated signals from both biotic and man-made sources. This combined material should provide interested readers with user-friendly resources for implementing warping methods into their own research.

Vibrations of a diaphragm of an acoustic stethoscope in contact with a body of an auscultated patient are the source of the sound transmitted to the ears of a physician performing examination. Mechanical properties of a diaphragm can thus significantly affect the parameters of the transmitted bioacoustic signals. However, the exact relation remains mostly unclear, as the underlying phenomena involve complex effects of acoustic coupling between the diaphragm and the body of a patient. The present study introduces a detailed methodology for determining vibroacoustic behavior of a diaphragm of a stethoscope during a heart auscultation. A laser Doppler vibrometer is used to measure the velocity of vibrations at various points on the surface of a diaphragm during the examination. Synchronized recordings of electrocardiography signals are used for segmentation. Representative data sets are selected and analyzed for various kinds of diaphragms. The results show significant differences in vibration velocity levels and their distribution across the surfaces of the considered structures, but no significant filtering effects.

A model is described in which the effects of articulatory movements to produce speech are generated by specifying relative acoustic events along a time axis. These events consist of directional changes of the vocal tract resonance frequencies that, when associated with a temporal event function, are transformed via acoustic sensitivity functions, into time-varying modulations of the vocal tract shape. Because the time course of the events may be considerably overlapped in time, coarticulatory effects are automatically generated. Production of sentence-level speech with the model is demonstrated with audio samples and vocal tract animations.

New technologies for the digital recording of broadband complex signals have made it possible to develop and create laboratory autonomous multichannel equipment to record bioacoustic activity on a digital medium with low power consumption and the possibility of continuous recording for up to 4 h. The equipment operates under the control of a fit-PC computer with the Windows operating system and the PowerGraph program for digital recording in a frequency band of the analog path of up to 600 kHz. Equipment testing recorded paradoxical signals from toothed whales with a frequency band exceeding 200 kHz. Signals with an extended frequency band were recorded in a laboratory experiment in open water while solving the problem of target tracking and in an experiment with provocation of acoustic communication behavior.

Previous studies suggested that frequency-modulated tonal stimuli where the frequency sweeps upward (up-chirps) may enhance auditory brainstem response (ABR) amplitudes in mammals. In this study, ABRs were measured in response to up-chirps in three killer whales (Orcinus orca) and compared to ABRs evoked by broadband clicks. Chirp durations ranged from 125 − 2000 μs. Chirp spectral content was either “uncompensated,” meaning the spectrum paralleled the transmitting response of the piezoelectric transducer, or “compensated,” where the spectral density level was flat (+/−4 dB) across the stimulus bandwidth (10 − 130 kHz). Compensated up-chirps consistently produced higher amplitude ABRs than uncompensated clicks with the same peak equivalent sound pressure level. ABR amplitude increased with up-chirp duration up to 1400 μs, although there was considerable variability between individuals. Results suggest that compensating stimuli for the response of transducers can have a dramatic effect on broadband ABRs, and that compensated up-chirps might be useful for testing whale species where large size makes far-field recording of ABRs at the skin surface difficult.Previous studies suggested that frequency-modulated tonal stimuli where the frequency sweeps upward (up-chirps) may enhance auditory brainstem response (ABR) amplitudes in mammals. In this study, ABRs were measured in response to up-chirps in three killer whales (Orcinus orca) and compared to ABRs evoked by broadband clicks. Chirp durations ranged from 125 − 2000 μs. Chirp spectral content was either “uncompensated,” meaning the spectrum paralleled the transmitting response of the piezoelectric transducer, or “compensated,” where the spectral density level was flat (+/−4 dB) across the stimulus bandwidth (10 − 130 kHz). Compensated up-chirps consistently produced higher amplitude ABRs than uncompensated clicks with the same peak equivalent sound pressure level. ABR amplitude increased with up-chirp duration up to 1400 μs, although there was considerable variability between individuals. Results suggest that compensating stimuli for the response of transducers can have a dramatic effect on broadband ABRs, a...

The auditory frequency weighted daily sound exposure level (SEL) is used in many jurisdictions to assess possible injury to the hearing of marine life. Therefore, using daily SEL to describe soundscapes would provide baseline information about the environment using the same tools used to measure injury. Here, the daily SEL from 12 recordings with durations of 18-97days are analyzed to: (1) identify natural soundscapes versus environments affected by human activity, (2) demonstrate how SEL accumulates from different types of sources, (3) show the effects of recorder duty cycling on daily SEL, (4) make recommendations on collecting data for daily SEL analysis, and (5) discuss the use of the daily SEL as an indicator of cumulative effects. The autocorrelation of the one-minute sound exposure is used to help identify soundscapes not affected by human activity. Human sound sources reduce the autocorrelation and add low-frequency energy to the soundscapes. To measure the daily SEL for all marine mammal auditory frequency weighting groups, data should be sampled at 64kHz or higher, for at least 1min out of every 30min. The daily autocorrelation of the one-minute SEL provides a confidence interval for the daily SEL computed with duty-cycled data. (C) 2019 Acoustical Society of America.

Emotion expression plays a crucial role for regulating social interactions. One efficient channel for emotion communication is the vocal-auditory channel, which enables a fast transmission of information. Filter-related parameters (formants) have been suggested as a key to the vocal differentiation of emotional valence (positive versus negative) across species, but variation in relation to emotions has rarely been investigated. Here, whether pig (Sus scrofa domesticus) closed-mouth grunts differ in source- and filter-related features when produced in situations assumed to be positive and negative is investigated. Behavioral and physiological parameters were used to validate the animals' emotional state (both in terms of valence and arousal, i.e., bodily activation). Results revealed that grunts produced in a positive situation were characterized by higher formants, a narrower range of the third formant, a shorter duration, a lower fundamental frequency, and a lower harmonicity compared to negative grunts. Particularly, formant-related parameters and duration made up most of the difference between positive and negative grunts. Therefore, these parameters have the potential to encode dynamic information and to vary as a function of the emotional valence of the emitter in pigs, and possibly in other mammals as well.

Significant effort has been made over the last few decades to develop automated passive acoustic monitoring (PAM) systems capable of classifying cetaceans at the species level. The utility of such systems depends on the systems' ability to operate across a wide range of ocean acoustic environments; however, anecdotal evidence suggests that site-specific propagation characteristics impact the performance of PAM systems. Variability in propagation characteristics leads to differences in how each cetacean vocalization is altered as it propagates along the source-receiver path. A propagation experiment was conducted in the Gulf of Mexico to investigate the range-dependent impacts of acoustic propagation on the performance of an automated classifier. Modified bowhead and humpback vocalizations were transmitted over ranges from 1 to 10 km. When the classifier was trained with signals collected near the sound source, it was found that the performance decreased with increasing transmission range-this appeared to be largely explained by decreasing signal-to-noise ratio (SNR). Generation of performance matrices showed that one method to develop a classifier that maintains high performance across many ranges is to include a varied assortment of ranges in the training data; however, if the training set is limited, it is best to train on relatively low SNR vocalizations.

Ultrasound (US) and microbubble (MB) interaction is an important factor in the research of bioacoustics, as well as targeted drug and gene delivery. In this study, we demonstrate the feasibility of pulse−echo M-mode imaging system to be used for the visualization and quantification of US–MB interaction in both spatial and temporal dimensions. The system incorporates an exposure chamber with the cell–MB suspension, a 2.7 MHz focused US transducer, a US pulser–receiver and the customized LabView software. The results of cell and MB interaction obtained after M-mode image analysis have showed the US–MB interaction to be non-uniform in space and non-stationary in time. In order to quantify the spatio-temporal US–MB interaction, we have introduced the time function of spatial homogeneity dynamics. We have observed that the effective duration of interaction can be characterized at the predefined threshold of spatial homogeneity. For example, at the US excitation of 360 kPa peak negative pressure (15 bursts transmitted at 80 Hz pulse repetition frequency), the US–MB interaction persists for more than 5 seconds in the range at 4 mm depth of the exposure chamber with more than 50% of homogeneity. The system proposed in this assay is feasible for the characterization of US–MB interaction and can be exploited to optimize the MB concentration and/or the US excitation parameters.

Detecting marine mammal vocalizations in underwater acoustic environments and classifying them to species level is typically an arduous manual analysis task for skilled bioacousticians. In recent years, machine learning and other automated algorithms have been explored for quickly detecting and classifying all sound sources in an ambient acoustic environment, but many of these still require a large training dataset compiled through time-intensive manual pre-processing. Here, an application of the signal decomposition technique Empirical Mode Decomposition (EMD) is presented, which does not require a priori knowledge and quickly detects all sound sources in a given recording. The EMD detection process extracts the possible signals in a dataset for minimal quality control post-processing before moving onto the second phase: the EMD classification process. The EMD classification process uniquely identifies and labels most sound sources in a given environment. Thirty-five recordings containing different marine mammal species and mooring hardware noises were tested with the new EMD detection and classification processes. Ultimately, these processes can be applied to acoustic index development and refinement.

In a previous experiment [Baddouret al., J. Acoust. Soc. Am.117(2), 934–943 (2005)] it was shown that it is possible to deduce the ultra-sound backscatter transfer function from single, subresolution cells in vitro, across a broad, continuous range of frequencies. Additional measurements have been performed at high frequencies (10 – 65 MHz) on cells with different relative nucleus sizes. It was found that for cells with a nucleus to cell volume ratio of 0.50, the backscatter response was better modeled as an elastic sphere. For the cells in which the ratio was 0.33, the backscatter showed good agreement with the theoretical solution for a fluid sphere.

Forced expiratory (FE) noise is a powerful bioacoustic signal containing information on human lung biomechanics. FE noise is attributed to a broadband part and narrowband components—forced expiratory wheezes (FEWs). FE respiratory noise is composed by acoustic and hydrodynamic mechanisms. An origin of the most powerful mid-frequency FEWs (400–600 Hz) is associated with the 0th–3rd levels of bronchial tree in terms of Weibel [(2009). Swiss Med. Wkly. 139(27–28), 375–386], whereas high-frequency FEWs (above 600 Hz) are attributed to the 2nd–6th levels of bronchial tree. The laboratory prototype of the apparatus is developed, which includes the electret microphone sensor with stethoscope head, a laptop with external sound card, and specially developed software. An analysis of signals by the new method, including FE time in the range from 200 to 2000 Hz and band-pass durations and energies in the 200-Hz bands evaluation, is applied instead of FEWs direct measures. It is demonstrated experimentally that developed FE acoustic parameters correspond to basic indices of lung function evaluated by spirometry and body plethysmography and may be even more sensitive to some respiratory deviations. According to preliminary experimental results, the developed technique may be considered as a promising instrument for acoustic monitoring human lung function in extreme conditions, including diving and space flights. The developed technique eliminates the contact of the sensor with the human oral cavity, which is characteristic for spirometry and body plethysmography. It reduces the risk of respiratory cross-contamination, especially during outpatient and field examinations, and may be especially relevant in the context of the COVID-19 pandemic.

Deep clustering was applied to unlabeled, automatically detected signals in a coral reef soundscape to distinguish fish pulse calls from segments of whale song. Deep embedded clustering (DEC) learned latent features and formed classification clusters using fixed-length power spectrograms of the signals. Handpicked spectral and temporal features were also extracted and clustered with Gaussian mixture models (GMM) and conventional clustering. DEC, GMM, and conventional clustering were tested on simulated datasets of fish pulse calls (fish) and whale song units (whale) with randomized bandwidth, duration, and SNR. Both GMM and DEC achieved high accuracy and identified clusters with fish, whale, and overlapping fish and whale signals. Conventional clustering methods had low accuracy in scenarios with unequal-sized clusters or overlapping signals. Fish and whale signals recorded near Hawaii in February-March 2020 were clustered with DEC, GMM, and conventional clustering. DEC features demonstrated the highest accuracy of 77.5% on a small, manually labeled dataset for classifying signals into fish and whale clusters., to appear in Journal of the Acoustical Society of America, April 2021

Bio-logging devices are advancing the understanding of marine animal behavior, but linking sound production and behavior of individual baleen whales is still unreliable. Tag placement potentially within the near field of the sound source creates uncertainty about how tagged animal sounds will register on recorders. This study used data from a tagged singing humpback whale to evaluate this question of how sound levels present on a tag when calls are produced by a tagged animal. Root-mean-square (rms) received levels (RLs) of song units ranged from 112 to 164 dB re 1 μPa rms, with some, but not all, of the lower frequency units registering on the tag's 800 Hz accelerometer sensor. Fifty-nine percent of recorded units measured lower acoustic RLs than previously reported source levels for humpback song, but signal-to-noise ratios (SNRs) were 30–45 dB during periods of the dive with low noise. This research highlights that tag RL does not alone predict caller identity, argues for higher SNR thresholds if using SNR to inform decisions about the source of a call, and provides a baseline for future research identifying diagnostic properties of tagged animal calls in cetacean bioacoustic tag datasets.

Although several bioacoustics investigations have shed light on the acoustic communication of Mediterranean fish species, the occurrence of fish sounds has never been reported below −40 m depth. This study assessed the occurrence of fish sounds at greater depths by monitoring the soundscape of a Mediterranean submarine canyon (Calvi, France) thanks to a combination of Static Acoustic Monitoring (three stations, from −125 to −150 m depth, 3 km from coastline) and of hydrophone-integrated gliders (Mobile Acoustic Monitoring; from −60 to −900 m depth, 3–6 km from coastline). Biological sounds were detected in 38% of the audio files; ten sound types (for a total of more than 9.000 sounds) with characteristics corresponding to those emitted by vocal species, or known as produced by fish activities, were found. For one of these sound types, emitter identity was inferred at the genus level (Ophidion sp.). An increase of from 10 to 15 dB re 1 μPa in sea ambient noise was observed during daytime hours due to boat traffic, potentially implying an important daytime masking effect. This study shows that monitoring the underwater soundscape of Mediterranean submarine canyons can provide holistic information needed to better understand the state and the dynamics of these heterogeneous, highly diverse environments.

Spatial localization based on acoustic observations is a rich field of interest in acoustic signal analysis. This special issue takes a close look at the diverse and growing range of problems in this area and the broad perspectives and methodologies that are presently being developed to solve them. The collection of articles presents recent advances in localization in complex and uncertain environments across a wide range of acoustic disciplines, from animal bioacoustics and acoustic signal processing in underwater environments to in air environments, architectural acoustics, and acoustic transduction.

An additional heating caused by the shear wave generated by an ultrasonic wave incident on a soft tissue boundary is considered for different tissue parameters and wave frequencies in the MHz range. The shear wave structure and the space-time dynamics of the temperature field are studied for three realistic examples of tissue parameters. It is shown that in viscous tissues the shear component can significantly contribute to the heating in a narrow layer near the boundary.

The perception of the build-up of auditory streaming has been widely investigated in humans, while it is unknown whether animals experience a similar perception when hearing high (H) and low (L) tonal pattern sequences. The paradigm previously used in European starlings (Sturnus vulgaris) was adopted in two experiments to address the build-up of auditory streaming in budgerigars (Melopsittacus undulatus). In experiment 1, different numbers of repetitions of low-high-low triplets were used in five conditions to study the build-up process. In experiment 2, 5 and 15 repetitions of high-low-high triplets were used to investigate the effects of repetition rate, frequency separation, and frequency range of the two tones on the birds' streaming perception. Similar to humans, budgerigars subjectively experienced the build-up process in auditory streaming; faster repetition rates and larger frequency separations enhanced the streaming perception, and these results were consistent across the two frequency ranges. Response latency analysis indicated that the budgerigars needed a longer amount of time to respond to stimuli that elicited a salient streaming perception. These results indicate, for the first time using a behavioral paradigm, that budgerigars experience a build-up of auditory streaming in a manner similar to humans.

International audience; Spiny lobsters (Palinurus elephas) have been overfished in European waters, and adult breeders are now scarce. Our recent study highlighted the high acoustic potential of this species, which can emit loud broadband pulse trains, called “antennal rasps,” with peak-to-peak source levels (estimated at 1 m from the source) above 160 dB re 1 μPa² [Jézéquel et al., Marine Ecology Progress Series 615 (2019)]. These acoustic properties imply that these sounds could be detected during in situ passive acoustic monitoring. However, before using a such tool, we need to understand how antennal rasps propagate in situ and at what distance they could be detected above the ambient noise. To answer these questions, we recorded spiny lobster antennal rasps in the Iroise Sea (Brittany, France). We used a linear array of 8 hydrophones, with distances between animals and receivers ranging from 0.5 m to 100 m. We recorded antennal rasps from 38 individuals of various sizes. Our results demonstrate that large spiny lobsters can be detected at 100 m, and that sound properties might be directly influenced by the size of the individuals.

International audience; In this paper, we introduce an algorithm for locating sound-producing fish in a small rectangular tank that can be used, e.g., in behavioral bioacoustical studies to determine which fish in a group is sound-producing. The technique consists in locating a single sound source in the tank using signals gathered by four hydrophones placed in the tank together with a group of fish under study. The localization algorithm used in this paper is based on a ratio of two spectra ratios: the spectra ratio between the sound pressure measured by hydrophones at two locations and the spectra ratio between the theoretical Green's functions at the same locations. The results are compared to a localization based on image processing technique and with video recordings acquired synchronously with the acoustic recordings.

At present, there are no direct measures of hearing for any baleen whale (Mysticeti). The most viable alternative to in vivo approaches to simulate the audiogram is through modeling outer, middle, and inner ear functions based on the anatomy and material properties of each component. This paper describes a finite element model of the middle ear for the humpback whale (Megaptera novaeangliae) to calculate the middle ear transfer function (METF) to determine acoustic energy transmission to the cochlea. The model was developed based on high resolution computed tomography imaging and direct anatomical measurements of the middle ear components for this mysticete species. Mechanical properties for the middle ear tissues were determined from experimental measurements and published values. The METF for the humpback whale predicted a better frequency range between approximately 15 Hz and 3 kHz or between 200 Hz and 9 kHz based on two potential stimulation locations. Experimental measures of the ossicular chain, tympanic membrane, and tympanic bone velocities showed frequency response characteristics consistent with the model. The predicted best sensitivity hearing ranges match well with known vocalizations of this species.

In this study, the echolocation and flight behaviors of the Taiwanese leaf-nosed bat (Hipposideros armiger terasensis), which uses constant-frequency (CF) biosonar signals combined with a frequency-modulated (FM) sweep, are compared with those of the big brown bat (Eptesicus fuscus), which uses FM signals alone. The CF-FM bat flew through a corridor bounded by vertical poles on either side, and the inter-pole spacing of the walls was manipulated to create different echo flow conditions. The bat's flight trajectories and echolocation behaviors across corridor conditions were analyzed. Like the big brown bat, the Taiwanese leaf-nosed bat centered its flight trajectory within the corridor when the pole spacing was the same on the two walls. However, the two species showed different flight behaviors when the pole spacing differed on the two walls. While the big brown bat deviated from the corridor center towards the wall with sparse pole spacing, the Taiwanese leaf-nosed bat did not. Further, in comparison to E. fuscus, H. a. terasensis utilized different echolocation patterns showing a prevalence of grouping sounds into clusters of three. These findings indicate that the two species' distinct sonar signal designs contribute to their differences in flight trajectories in a structured corridor.

In this paper, acoustic analysis of misarticulated trills in cleft lip and palate speakers is carried out using excitation source based features: strength of excitation and fundamental frequency, derived from zero-frequency filtered signal, and vocal tract system features: first formant frequency (F1) and trill frequency, derived from the linear prediction analysis and autocorrelation approach, respectively. These features are found to be statistically significant while discriminating normal from misarticulated trills. Using acoustic features, dynamic time warping based trill misarticulation detection system is demonstrated. The performance of the proposed system in terms of the F1-score is 73.44%, whereas that for conventional Mel-frequency cepstral coefficients is 66.11%.

Sounds produced by Arctic cod were recorded for the first time and suggest passive acoustic monitoring (PAM) can be an effective additional tool for the study and management of the species. Each of the 38 calls detected in three different aquatic facilities consisted of a single grunt with 6 to 12 pulses and a mean duration of 289 ms. Call frequency ranged between 59 and 234 Hz, with a mean peak frequency of 107 Hz. These preliminary data suggest Arctic cod can be distinguished from other gadids, but additional studies of sympatric species are needed before PAM can be confidently adopted.

Funding: U.S. Office of Naval Research (ONR Grant No. N00014-14-1-0409); P.L.T. acknowledges funding received from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (Grant No. HR09011) and contributing institutions. Passive acoustic monitoring with widely-dispersed hydrophones has been suggested as a cost-effective method to monitor population densities of echolocating marine mammals. This requires an estimate of the area around each receiver over which vocalizations are detected—the “effective detection area” (EDA). In the absence of auxiliary measurements enabling estimation of the EDA, it can be modelled instead. Common simplifying model assumptions include approximating the spectrum of clicks by flat energy spectra, and neglecting the frequency-dependence of sound absorption within the click bandwidth (narrowband assumption), rendering the problem amenable to solution using the sonar equation. Here, it is investigated how these approximations affect the estimated EDA and their potential for biasing the estimated density. EDA was estimated using the passive sonar equation, and by applying detectors to simulated clicks injected into measurements of background noise. By comparing model predictions made using these two approaches for different spectral energy distributions of echolocation clicks, but identical click source energy level and detector settings, EDA differed by up to a factor of 2 for Blainville's beaked whales. Both methods predicted relative density bias due to narrowband assumptions ranged from 5% to more than 100%, depending on the species, detector settings, and noise conditions. Publisher PDF

Echolocating bats can rapidly modify frequency modulation (FM) curvatures of their calls when facing challenging echolocation tasks. Frequency parameters, such as start/end/peak frequency, have often been extracted from the time-frequency domain to study the call variation. Even though this kind of signal investigation method reveals important findings, these approaches to analyze bat echolocation calls use bulk parameters, which hide subtleties in the call structure that may be important to the bat. In some cases, calls can have the same start and end frequencies but have different FM curvatures, and subsequently may influence the sensory task performance. In the present study, the authors demonstrate an algorithm using a combination of digital filters, power limited time-frequency information, derivative dynamic time warping, and agglomerative hierarchical clustering to extract and categorize the time-frequency components (TFCs) of 21 calls from Brazilian free-tailed bat (Tadarida brasiliensis) to quantitatively compare FM curvatures. The detailed curvature analysis shows an alternative perspective to look into the TFCs and hence serves as the preliminary step to understand the adaptive call design of bats.

This paper considers the importance of particle motion to fishes and invertebrates and the steps that need to be taken to improve knowledge of its effects. It is aimed at scientists investigating the impacts of sounds on fishes and invertebrates but it is also relevant to regulators, those preparing environmental impact assessments, and to industries creating underwater sounds. The overall aim of this paper is to ensure that proper attention is paid to particle motion as a stimulus when evaluating the effects of sound upon aquatic life. Directions are suggested for future research and planning that, if implemented, will provide a better scientific basis for dealing with the impact of underwater sounds on marine ecosystems and for regulating those human activities that generate such sounds. The paper includes background material on underwater acoustics, focusing on particle motion; the importance of particle motion to fishes and invertebrates; and sound propagation through both water and the substrate. Consideration is then given to the data gaps that must be filled in order to better understand the interactions between particle motion and aquatic animals. Finally, suggestions are provided on how to increase the understanding of particle motion and its relevance to aquatic animals.

Branstetter and his colleagues present the audiograms of eight killer whales and provide a comprehensive review of previous killer whale audiograms. In their paper, they say that the present authors have reported a relationship between size and high-frequency hearing but that echolocating cetaceans might be a special case. The purpose of these comments is to clarify that the relationship of a species' high-frequency hearing is not to its size (mass) but to its "functional interaural distance" (a measure of the availability of sound-localization cues). Moreover, it has previously been noted that echolocating animals, cetaceans as well as bats, have extended their high-frequency hearing somewhat beyond the frequencies used by comparable non-echolocators for passive localization.

Vibratory pile drivers, used for marine construction, can produce sustained, high sound pressure levels (SPLs) in areas that overlap with dolphin habitats. Dolphins rely on echolocation for navigation, detecting predators and prey, and to coordinate group behavior. This study examined the effects of vibratory pile driver noise on dolphin sustained target detection capabilities through echolocation. Five dolphins were required to scan their enclosure and indicate the occurrences of phantom echoes during five different source levels of vibratory pile driver playback sound (no-playback control, 110, 120, 130, and 140 dB re 1 μPa). Three of the dolphins demonstrated a significant decrease in target detection performance at 140 dB playback level that was associated with an almost complete secession of echolocation activity. The performance of two dolphins was not affected. All dolphins rapidly returned to baseline levels of target detection performance by their second replication. However, an increased number of clicks was produced at the highest playback SPL. The data suggest that the decrease in vigilant behavior was due to the vibratory pile driver noise distracting the dolphins and decreasing their motivation to perform the task.

The use of broadband acoustic sonars in acoustical oceanography studies in a broad range of environments, particularly for ecological studies, has rapidly overtaken the use of traditional narrowband sonar systems. Almost as importantly, and often underrecognized, is the diversity of platforms that have been used to support these measurements. In this talk, we present broadband acoustic backscatter collected from a number of platforms, including AUVs, a variety of towed and profiling systems, and a bioacoustics mooring. The primary focus of this talk will be on the data collected from REMUS 600 and REMUS 100 AUVs, and the types of science questions that these platforms, outfitted with broadband scientific echosounders, best support. This work was supported by the Ocean Acoustics and Task Force Ocean Programs at the Office of Naval Research.

Bioacoustics play a major role in the field of ornithology, ecology, animal behaviour study, habitat monitoring, species conservation and design of deterrent system. This work focusses on the design and implementation and performance evaluation of an automatic, more efficient and flexible bird sound based recognition system for classifying eight species of popular Eurasian birds the standard online annotated databases. A dedicated virtual instrument tool with effective GUI is developed that acquires, pre-processes the sound samples and generates statistically evaluated short term Fourier transform spectrogram based feature matrix, suited for characterization of vocalization patterns of bird species. Using the well-labelled feature data of sound records, multi-layer perceptron artificial neural network (MLP-NN) classifier model is designed, trained, tested and optimized using feedforward-backpropagation supervised learning algorithm. Various experiments, following a systematic approach, are conducted to optimize the structure of MLP with respect of number of neurons in the hidden layer, epochs and learning rate for attaining enhanced recognition accuracy (96.1%), recall (82.6%) and precision (84.5%). The performance of the optimal model is also analysed in terms of recognition capabilities of individual bird species that indicate promising results. A few of the birds are recognized accurately and precisely when present as compared to the others. The tendency of the model to wrongly identify or miss bird species also remained low. The model performance over the unseen dataset also remained satisfactory with cross-validation classification accuracy of 81.4%. The system being scalable, can easily be reused in future to retrain the model over the large set of sound samples from real world recordings with improved acoustic features for achieving very high classification accuracy and reliability.

Funding: the Mexican Government (through the Mexican Secretaría de Medio Ambiente y Recursos Naturales), especially Minister R. Pacchiano and A. Michel; U.S. Marine Mammal Commission, in particular T. Ragen, R. Lent, and P. Thomas; the World Wildlife Fund (WWF) Mexico, in particular O. Vidal and E. Sanjurjo; Le Equipe Cousteau; The Ocean Foundation; Fonds de Dotation pour la Biodiversité; MAAF Assurances (Save Your Logo); WWF-US; Opel Project Earth; Fideicomiso Fondo para la Biodiversidad; Instituto Nacional de Ecología y Cambio Climático; Comisión Nacional de Áreas Naturales Protegidas; and Directorate of the Reserva de la Biósfera Alto Golfo de California y Delta del Río Colorado. The vaquita is a critically endangered species of porpoise. It produces echolocation clicks, making it a good candidate for passive acoustic monitoring. A systematic grid of sensors has been deployed for 3 months annually since 2011; results from 2016 are reported here. Statistical models (to compensate for non-uniform data loss) show an overall decline in the acoustic detection rate between 2015 and 2016 of 49% (95% credible interval 82% decline to 8% increase), and total decline between 2011 and 2016 of over 90%. Assuming the acoustic detection rate is proportional to population size, approximately 30 vaquita (95% credible interval 8–96) remained in November 2016. Publisher PDF

In 2014, Indo-Pacific humpback dolphins were recorded for the first time in waters southwest of Hainan Island, China. In this paper, the temporal occurrence of Indo-Pacific humpback dolphins in this region was detected by stationary passive acoustic monitoring. During the 130-day observation period (from January to July 2016), 1969 click trains produced by Indo-Pacific humpback dolphins were identified, and 262 ten-minute recording bins contained echolocation click trains of dolphins, of which 70.9% were at night and 29.1% were during the day. A diurnal rhythm with a nighttime peak in acoustic detections was found. Passive acoustic detections indicated that the Indo-Pacific humpback dolphins frequently occurred in this area and were detected mainly at night. This information may be relevant to conservation efforts for these dolphins in the near future.

Snapping shrimp are perhaps the most pervasive sources of biological sound in the ocean. The snapping sounds of cryptic shrimp colonies in shallow coastal habitats worldwide create a near-continuous crackling with high spatiotemporal variability, yet the underlying acoustic ecology is not well understood. This study investigated sound production rates and acoustic behavior of snapping shrimp species common in the Western Atlantic Ocean and Gulf of Mexico (Alpheus heterochaelis and Alpheus angulosus). Snap rates were measured in a controlled laboratory setting under natural light, temperature, and substrate conditions for shrimp held individually, in pairs, and in a ten-shrimp mesocosm, to test hypotheses that acoustic activity varies with time-of-day and social context. Spontaneous snapping was observed for 81 out of 84 solitary shrimp monitored. Time-of-day influenced snap output for individuals and same-sex pairs-higher rates occurred during dusk and night, compared to daylight hours, but this pattern was inconsistent for opposite-sex pairs and a mixed-sex group. These laboratory results provide insight into behavioral rhythms that may influence snapping patterns in natural populations, and underscore the limited understanding of a major sound source in marine environments.

In this paper, an extracorporeal shock wave source composed of small ellipsoidal sparker units is described. The sparker units were arranged in an array designed to produce a coherent shock wave of sufficient strength to fracture kidney stones. The objective of this paper was to measure the acoustical output of this array of 18 individual sparker units and compare this array to commercial lithotripters. Representative waveforms acquired with a fiber-optic probe hydrophone at the geometric focus of the sparker array indicated that the sparker array produces a shock wave (P+ ∼40–47 MPa, P− ∼2.5–5.0 MPa) similar to shock waves produced by a Dornier HM-3 or Dornier Compact S. The sparker array's pressure field map also appeared similar to the measurements from a HM-3 and Compact S. Compared to the HM-3, the electrohydraulic technology of the sparker array produced a more consistent SW pulse (shot-to-shot positive pressure value standard deviation of ±4.7 MPa vs ±3.3 MPa).

Acoustic signal production is affected by allometric relationships, by which the larger the animal, the lower its call frequency. In this paper, three evolutionary acoustic hypotheses were tested: the Signal-to-Noise Ratio Hypothesis (SNRH), in which evolution maximizes call ranges by increasing the signal-to-noise ratio; the Stimulus Threshold Hypothesis (STH), in which evolution maximizes the range of a specific signal threshold; and the Body Size Hypothesis (BSH), in which the emission of long wavelengths is enabled by body size. Three spectral metrics were measured, Dominant Frequency (FDOM), Minimum Fundamental Frequencies (FFMIN), and Maximum Fundamental Frequencies (FFMAX) of Neotropical Parrots, New World Doves, Woodcreepers, Tinamous, and Thrushes. A Ranged Major Axis (RMA) regression showed that body mass is significantly correlated with all of the spectral parameters in Parrots, Doves, and Woodcreepers, but only with the fundamental frequencies of Tinamous. The FDOM of Parrots corroborated the SNRH. The FFMIN of Woodcreepers and Tinamous corroborated the SNRH and BSH. The FFMAX of Parrots corroborated the STH and BSH. Those acoustic hypotheses could shed light on the evolutionary processes involved in avian communication, although results indicate that these depend on the taxa and spectral parameters considered.

The paper discusses the research areas which are placed in the modern science on the borderline between ecology and acoustics. It is explained what ideas are included in the concepts of bioacoustics, acoustic ecology, ecoacoustics, and soundscape ecology. The results obtained in these domains are compared with those received in vibroacoustic research presented at the Polish WIBROTECH conference cycle. The paper suggests an inventory of research topics connected with acoustics and ecology. In the second part of the paper the author presents the possibilities of ambisonic technology of recording and listening to sounds for the analysis in bioacoustic research.

International audience; Since passive acoustic monitoring is widely used, unidentified acoustic signals from marine mammals are commonly reported. The signal characteristics and emission patterns are the main clues to identify the possible sources. In this study, the authors describe two previously unidentified sounds, recorded at up to five widely-spaced sites (30 × 30 degree area) in the southern Indian Ocean, in 2007 and between 2010 and 2015. The first reported signal (M-call) consists of a single tonal unit near 22 Hz and lasting about 10 s, repeated with an interval longer than 2 min. This signal is only detected in 2007. The second signal (P-call) is also a tonal unit of 10 s, repeated every 160 s, but at a frequency near 27 Hz. Its yearly number increased greatly between 2007 and 2010, and moderately since then. Based on their characteristics and seasonal patterns, this study shows that both signals are clearly distinct from any known calls of blue whale subspecies and populations dwelling in the southern Indian Ocean. However, they display similarities with blue whale vocalizations. More particularly, the P-call can be mistaken for the first tonal unit of the Antarctic blue whale Z-call.

Acoustic surveys of vocal animals can have significant advantages over visual surveys, particularly for marine mammals. For acoustic density estimates to be viable, however, the vocal output of the animals surveyed needs to be determined under a range of conditions and shown to be a robust predictor of abundance. In this study, the songs of humpback whales, one of the most vocal and best studied species of marine mammals, were tested as predictors of abundance. Two acoustic metrics, the number of singing whales and amount of songs produced, were compared with the number of whales seen traversing a study site on the eastern coast of Australia over an 18 year period. Although there were predictive relationships between both metrics and numbers of passing whales, these relationships changed significantly as the population grew in size. The proportion of passing whales that sang decreased as the population increased. Singing in humpback whales, therefore, is a poor predictor even of relative abundance and illustrates the caution required when developing acoustic survey techniques particularly when using social vocalizations.

Auditory brainstem response (ABR) measurements using conventional averaging (i.e., constant interstimulus interval, ISI) are limited to stimulus rates low enough to prevent overlapping of the ABRs to successive stimuli. To overcome this limitation, stimuli may be presented at high rates using pseudorandom sequences (e.g., maximum length sequences) or quasi-periodic sequences; however, these methods restrict the available stimulus sequences and require deconvolution to recover the ABR from the overlapping responses. Randomized stimulation and averaging (RSA) is an alternate method where evoked responses at high rates are obtained by averaging responses to stimuli with ISIs drawn from a random distribution. The RSA method enables precise control over stimulus jitter, is flexible with respect to stimulus sequence parameters, and does not require deconvolution to extract the ABR waveform. In this study, ABRs were measured in three normal-hearing dolphins using conventional averaging and RSA. Results show the RSA method to be effective in dolphins if the ISI jitter ≥ ∼1.5 ms and that the influence of stimulus artifacts in the averaged ABR can be substantially reduced by alternating stimulus polarity on successive presentations rather than employing digital blanking or iterative processes.

To study intra-species variability in audiograms, the hearing sensitivity of a six-year-old female and a three-year-old male harbor porpoise was measured by using a standard psycho-acoustic technique under low ambient noise conditions. The porpoises' hearing thresholds for 13 narrow-band sweeps with center frequencies between 0.125 and 150 kHz were established. The resulting audiograms were U-shaped and similar. The main difference (25 dB) in mean thresholds between the two porpoises was at the high-frequency end of the hearing range (at 150 kHz). Maximum sensitivity (47 dB re 1 μPa for the female and 44 dB re 1 μPa for the male) occurred at 125 kHz. The range of most sensitive hearing (defined as within 10 dB of maximum sensitivity) was from 16 to ∼140 kHz. Sensitivity declined sharply above 125 kHz. All five porpoises for which a valid behavioral audiogram now exists were rehabilitated stranded animals, all were tested with similar psycho-acoustic techniques, and all had similar audiograms. The present study provides further evidence to confirm that the hearing range and sensitivity of the first three harbor porpoises, which have been used in secondary research and on which policy decisions have been based, are representative of those of young harbor porpoises in general.