1. Mutations in gyrA and parC genes in fluoroquinolone-resistant Acinetobacter baumannii that causes hospital acquired infection.
- Author
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Taha, Marwa S., Shoeib, Sarah M., and Abdelwahab, Marwa A.
- Subjects
FLUOROQUINOLONES ,ACINETOBACTER baumannii ,POLYMERASE chain reaction ,DNA topoisomerase II ,HOSPITAL care - Abstract
Background: Acinetobacter baumannii, are involved in hospital-acquired infections and are increasingly developing resistance to fluoroquinolones, such as ciprofloxacin. The most common method of fluoroquinolone resistance is alteration in genes that encode DNA gyrase (gyrA) and topoisomerase IV (parC).Methods: We sought to isolate fluoroquinolone-resistant A. baumannii and search for changes in gyrA (Ser83Leu) and parC (Ser80Leu) loci by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Results: We found that all 68 A. baumannii isolates that were part of this research were multidrug resistant and harbored gyrA and parC loci. Most isolates had ciprofloxacin minimal inhibitory concentrations of >128 µg/mL (50.8%). Mutations in gyrA were the most prevalent (47.45%), followed by parC mutations (33.9%) and combined mutations in both genes (23.7%).Conclusions: Single mutations in either gyrA (Ser83Leu) or parC (Ser80Leu) genes may be attributed to fluroquinolone resistance in A. baumannii. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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