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Your search keyword '"Kleczyńska, A."' showing total 3 results
Start Over Author "Kleczyńska, A." Topic acid phosphatase
3 results on '"Kleczyńska, A."'

1. The Comparison of Physicochemical Parameters, Antioxidant Activity and Proteins for the Raw Local Polish Honeys and Imported Honey Blends

Author

Michał Miłek, Aleksandra Bocian, Ewelina Kleczyńska, Patrycja Sowa, and Małgorzata Dżugan

Subjects
honey, quality standards, protein, amylase, acid phosphatase, native PAGE, Organic chemistry, QD241-441
Abstract

Many imported honeys distributed on the Polish market compete with local products mainly by lower price, which can correspond to lower quality and widespread adulteration. The aim of the study was to compare honey samples (11 imported honey blends and 5 local honeys) based on their antioxidant activity (measured by DPPH, FRAP, and total phenolic content), protein profile obtained by native PAGE, soluble protein content, diastase, and acid phosphatase activities identified by zymography. These indicators were correlated with standard quality parameters (water, HMF, pH, free acidity, and electrical conductivity). It was found that raw local Polish honeys show higher antioxidant and enzymatic activity, as well as being more abundant in soluble protein. With the use of principal component analysis (PCA) and stepwise linear discriminant analysis (LDA) protein content and diastase number were found to be significant (p < 0.05) among all tested parameters to differentiate imported honey from raw local honeys.

Published
2021
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2. The Comparison of Physicochemical Parameters, Antioxidant Activity and Proteins for the Raw Local Polish Honeys and Imported Honey Blends

Author

Ewelina Kleczyńska, Aleksandra Bocian, Patrycja Sowa, Michał Miłek, and Małgorzata Dżugan

Subjects
0106 biological sciences, amylase, Antioxidant, native PAGE, Chemical Phenomena, DPPH, medicine.medical_treatment, Pharmaceutical Science, Organic chemistry, Protein profile, honey, Native page, 01 natural sciences, Antioxidants, Article, Analytical Chemistry, Structure-Activity Relationship, chemistry.chemical_compound, 0404 agricultural biotechnology, QD241-441, Drug Discovery, medicine, Zymography, Food science, Amylase, Physical and Theoretical Chemistry, biology, Chemistry, digestive, oral, and skin physiology, fungi, Acid phosphatase, Proteins, food and beverages, 04 agricultural and veterinary sciences, 040401 food science, Enzymes, Diastase, 010602 entomology, Chemistry (miscellaneous), acid phosphatase, biology.protein, Molecular Medicine, protein, quality standards
Abstract

Many imported honeys distributed on the Polish market compete with local products mainly by lower price, which can correspond to lower quality and widespread adulteration. The aim of the study was to compare honey samples (11 imported honey blends and 5 local honeys) based on their antioxidant activity (measured by DPPH, FRAP, and total phenolic content), protein profile obtained by native PAGE, soluble protein content, diastase, and acid phosphatase activities identified by zymography. These indicators were correlated with standard quality parameters (water, HMF, pH, free acidity, and electrical conductivity). It was found that raw local Polish honeys show higher antioxidant and enzymatic activity, as well as being more abundant in soluble protein. With the use of principal component analysis (PCA) and stepwise linear discriminant analysis (LDA) protein content and diastase number were found to be significant (p &lt, 0.05) among all tested parameters to differentiate imported honey from raw local honeys.

Published
2021

3. The Comparison of Physicochemical Parameters, Antioxidant Activity and Proteins for the Raw Local Polish Honeys and Imported Honey Blends.

Author

Miłek, Michał, Bocian, Aleksandra, Kleczyńska, Ewelina, Sowa, Patrycja, Dżugan, Małgorzata, Sanna, Gavino, Ciulu, Marco, Picò, Yolanda, Spano, Nadia, and Tuberoso, Carlo I.G.

Subjects
HONEY, FISHER discriminant analysis, ACID phosphatase, ANTIOXIDANTS, PRINCIPAL components analysis, OLIVE oil, AMYLASES
Abstract

Many imported honeys distributed on the Polish market compete with local products mainly by lower price, which can correspond to lower quality and widespread adulteration. The aim of the study was to compare honey samples (11 imported honey blends and 5 local honeys) based on their antioxidant activity (measured by DPPH, FRAP, and total phenolic content), protein profile obtained by native PAGE, soluble protein content, diastase, and acid phosphatase activities identified by zymography. These indicators were correlated with standard quality parameters (water, HMF, pH, free acidity, and electrical conductivity). It was found that raw local Polish honeys show higher antioxidant and enzymatic activity, as well as being more abundant in soluble protein. With the use of principal component analysis (PCA) and stepwise linear discriminant analysis (LDA) protein content and diastase number were found to be significant (p < 0.05) among all tested parameters to differentiate imported honey from raw local honeys. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

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