1. Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP.
- Author
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Křupka M, Mašek J, Bartheldyová E, Turánek Knötigová P, Plocková J, Korvasová Z, Škrabalová M, Koudelka Š, Kulich P, Zachová K, Czerneková L, Strouhal O, Horynová M, Šebela M, Miller AD, Ledvina M, Raška M, and Turánek J
- Subjects
- Acetylmuramyl-Alanyl-Isoglutamine toxicity, Animals, Calorimetry, Differential Scanning, Chelating Agents toxicity, Drug Carriers toxicity, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Light, Liposomes, Lyme Disease Vaccines administration & dosage, Mice, Mice, Inbred BALB C, Microscopy, Electron, Transmission, Nanoparticles toxicity, Scattering, Radiation, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Acetylmuramyl-Alanyl-Isoglutamine chemistry, Antibodies, Bacterial blood, Antigens, Bacterial immunology, Bacterial Outer Membrane Proteins immunology, Borrelia burgdorferi immunology, Chelating Agents chemistry, Drug Carriers chemistry, Lyme Disease Vaccines immunology, Nanoparticles chemistry
- Abstract
Lyme disease caused by spirochete Borrelia burgdorferi sensu lato, is a tick-born illness. If the infection is not eliminated by the host immune system and/or antibiotics, it may further disseminate and cause severe chronic complications. The immune response to Borrelia is mediated by phagocytic cells and by Borrelia-specific complement-activating antibodies associated with Th1 cell activation. A new experimental vaccine was constructed using non-lipidized form of recombinant B. burgdorferi s.s. OspC protein was anchored by metallochelating bond onto the surface of nanoliposomes containing novel nonpyrogenic lipophilized norAbuMDP analogues denoted MT05 and MT06. After i.d. immunization, the experimental vaccines surpassed Alum with respect to OspC-specific titers of IgG2a, IgG2b isotypes when MT06 was used and IgG3, IgM isotypes when MT05 was used. Both adjuvants exerted a high adjuvant effect comparable or better than MDP and proved themselves as nonpyrogenic., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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