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1. Fabrication of a "Selenium Signature" Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry.

Author

Chen Z, Shi Q, Wang W, Jiang Z, Zhang GL, Tong L, Mu X, and Tang B

Subjects
Biosensing Techniques, Cysteine analogs & derivatives, Humans, Mass Spectrometry, Acetylcysteine analysis, Cysteine analysis, Dipeptides analysis, Fluorescent Dyes chemistry, Glutathione analysis, Paper, Selenium chemistry
Abstract

Significant efforts have been made to develop robust and reliable methods for simultaneous biothiols determination in different matrices, but there still exist the problems such as easy oxidation, tedious derivatization, and difficulty in discrimination, which brings unsatisfactory results in their accuracy and fast quantification in biological samples. To overcome these problems, a simultaneous biothiols detection method combining a "selenium signature" chemical probe and paper spray mass spectrometry (PS-MS) was proposed. In the strategy, the modified-paper substrate is used to enhance the analytical performance. Chemical probe Ebselen-NH 2 that has a specific response to biothiols was designed and covalently fixed on the surface of an oxidized paper substrate. By the identification of derivatized product with distinctive selenium isotope distribution and employment of the optimized PS-MS method, qualitative and quantitative analysis of five biothiols including glutathione (GSH), cysteine (Cys), cysteinylglycine (CysGly), N-acetylcysteine (Nac), and homocysteine (Hcy) were realized. Biothiols in plasma and cell lysates were measured with satisfactory results. The established method not only provides a novel protocol for simultaneous determination of biothiols, but also is helpful for understanding the biological and clinical roles played by these bioactive small molecules.

Published
2021
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3. Fabrication of a 'Selenium Signature' Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry

Author

Weiqing Wang, Xiaoyan Mu, Zhongyao Jiang, Guanglu Zhang, Zhenzhen Chen, Bo Tang, Qian Shi, and Lili Tong

Subjects
Paper, Fabrication, Chromatography, Substrate (chemistry), chemistry.chemical_element, Biosensing Techniques, Dipeptides, Mass spectrometry, Small molecule, Glutathione, Mass Spectrometry, Analytical Chemistry, Acetylcysteine, chemistry.chemical_compound, Isotopes of selenium, Selenium, chemistry, Humans, Cysteine, Derivatization, Quantitative analysis (chemistry), Fluorescent Dyes
Abstract

Significant efforts have been made to develop robust and reliable methods for simultaneous biothiols determination in different matrices, but there still exist the problems such as easy oxidation, tedious derivatization, and difficulty in discrimination, which brings unsatisfactory results in their accuracy and fast quantification in biological samples. To overcome these problems, a simultaneous biothiols detection method combining a "selenium signature" chemical probe and paper spray mass spectrometry (PS-MS) was proposed. In the strategy, the modified-paper substrate is used to enhance the analytical performance. Chemical probe Ebselen-NH2 that has a specific response to biothiols was designed and covalently fixed on the surface of an oxidized paper substrate. By the identification of derivatized product with distinctive selenium isotope distribution and employment of the optimized PS-MS method, qualitative and quantitative analysis of five biothiols including glutathione (GSH), cysteine (Cys), cysteinylglycine (CysGly), N-acetylcysteine (Nac), and homocysteine (Hcy) were realized. Biothiols in plasma and cell lysates were measured with satisfactory results. The established method not only provides a novel protocol for simultaneous determination of biothiols, but also is helpful for understanding the biological and clinical roles played by these bioactive small molecules.

Published
2020