1. Acetylcholinesterase inhibition studies of alkaloid components from Crinum asiaticum var. sinicum : in vitro assessments by molecular docking and molecular dynamics simulations.
- Author
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Duc NV, Trang VT, Tuan Anh HL, Vinh LB, Phong NV, Thuan TQ, Hieu NV, Dat NT, Nhan LV, Tuan DT, Tuan Anh L, Thao DT, Tai BH, Cuong NC, Lien LQ, and Yang SY
- Subjects
- Molecular Structure, Cholinesterase Inhibitors pharmacology, Cholinesterase Inhibitors chemistry, Alkaloids chemistry, Alkaloids pharmacology, Molecular Docking Simulation, Acetylcholinesterase drug effects, Acetylcholinesterase metabolism, Molecular Dynamics Simulation, Crinum chemistry
- Abstract
Alkaloids are among the most important and best-known secondary metabolites as sources of new drugs from medicinal plants and marine organisms. A phytochemical investigation of the whole plant of Crinum asiaticum var. sinicum resulted in the isolation of seven alkaloids ( 1 - 7 ), including one new dimeric compound, bis-(-)-8-demethylmaritidine ( 1 ). Their structures were elucidated using NMR and HR-ESI-MS. The absolute configuration of new compound 1 was established by circular dichroism spectroscopy. All isolated compounds were evaluated for their inhibitory effects on acetylcholinesterase (AChE) activity in vitro . Among them, compound 1 exhibited the most potent AChE inhibition. Moreover, molecular docking and molecular dynamics simulations were carried out for the most active compound to investigate their binding interactions and dynamics behavior of the AChE protein-ligand complex. Therefore, compound 1 may be a potential candidate for effectively treating Alzheimer's disease.
- Published
- 2024
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