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Your search keyword '"Pyruvate Carboxylase immunology"' showing total 1 results
Start Over Descriptor "Pyruvate Carboxylase immunology" Topic acetyl-coa carboxylase

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1 results on '"Pyruvate Carboxylase immunology"'

1. A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases.

Author

McKeon C and Wolf B

Subjects
Animals, Avidin, Chromatography, Affinity, Humans, Infant, Ligases isolation & purification, Methods, Methylmalonyl-CoA Decarboxylase, Propionates immunology, Rabbits immunology, Acetyl-CoA Carboxylase immunology, Biotin pharmacology, Carbon-Carbon Ligases, Carboxy-Lyases immunology, Ligases immunology, Mitochondria, Liver enzymology, Pyruvate Carboxylase immunology
Abstract

Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purify the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.

Published
1982
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