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[Objective] To establish methods to determine the content of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine in Aconitun sungpanense Hand-Mazz. and provide scientific basis for its reasonable and safe application. [Methods] Aconitine, Yunaconitine, Crassicauline and Vilmorrianine were used as references. Phenomenex Gemini C18 110A (250 mm ×4.6 mm, 5 [μm); mobile phase; acetonitrile -0.2% acetic acid (pH was regulated to by triethylamine); gradient elute; flow rate; 1 mL/min; UV wavelength 235/254 nm; column temperature; 30 CC. [Results] The content of the 4 Ester-series alkaloids in Aconitun sungpanense Hand. -Mazz. could be determined at the same time. The average recovery rate (n = 6) of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine reached 99. 65% (RSD = 1. 46%), 99. 13% (RSD = 1.32%), 98.13% (RSD =0.98%) and 98.66% (RSD = 1.67%). [Conclusions] The method was simple, accurate and had good repeatability. It could determine the content of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine in Aconitun sungpanense Hand.-Mazz. at the same time. It also provided scientific basis for standardizing the processing of Aconitun sungpanense Hand. -Mazz. and its reasonable and safe application. [ABSTRACT FROM AUTHOR]