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Objective. To determine the test-retest reliability and validity of the Spanish version of the short-form International Physical Activity Questionnaire (IPAQ) among adults in Mexico.Methods. This was a cross-sectional study of a convenience sample of 267 adult factory workers in Mexico City. Participants were 19–68 years of age; 48% were female. Participants wore an accelerometer for 9 consecutive days and were administered the Spanish version of the short form IPAQ on two occasions (IPAQ1 and IPAQ2, separated by 9 days). The relation and differences between moderate-to-vigorous physical activity (MVPA) measures obtained from IPAQ1, IPAQ2, and the accelerometer were determined using correlations, linear regression, and paired t-tests. Results. IPAQ1 and IPAQ2 measures of MVPA were significantly correlated to each other (r = 0.55, P < 0.01). However, MVPA was 44 ± 408 minutes/week lower in IPAQ1 than in IPAQ2, although this difference did not reach statistical significance (P = 0.08). The (min/week) measures from IPAQ1 and IPAQ2 were only modestly correlated with the accelerometer measures (r = 0.26 and r = 0.31, P < 0.01), and by comparison to accelerometer measures, MVPA values were higher when based on IPAQ1 (174 ± 357 min/week, P < 0.01) than for IPAQ2 (135 ± 360 min/week, P < 0.01). The percentage of participants who were classified as physically inactive according to the World Health Organization guidelines was 18.0% in IPAQ1, 25.1% in IPAQ2, and 28.2% based on the accelerometer.Conclusions. Similar to what has been observed in other populations, the short form IPAQ has a modest reliability and poor validity for assessing MVPA among Mexican adults. [ABSTRACT FROM AUTHOR]