1. Parasitemia Induces High Plasma Levels of Interleukin-17 (IL-17) and Low Levels of Interleukin-10 (IL-10) and Transforming Growth Factor-β (TGF-β) in Pregnant Mice Infected with Malaria.
- Author
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RAHMAH, Zainabur, SASMITO, Sujarot Dwi, SISWANTO, Budi, SARDJONO, Teguh Wahju, and FITRI, Loeki Enggar
- Subjects
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ACADEMIC medical centers , *ANIMAL experimentation , *BIOLOGICAL models , *CYTOKINES , *ENZYME-linked immunosorbent assay , *GROWTH factors , *INTERLEUKINS , *LONGITUDINAL method , *MALARIA , *MICE , *T-test (Statistics) , *DATA analysis software , *PARASITEMIA , *PREGNANCY ,IMMUNE system physiology - Abstract
Background: During pregnancy, the balanced dominance of the T helper17 response shifts to a Th2 response that is characterised by the production of IL-10, following the completion of the implantation process. Transforming growth factor-β (TGF-β) expression is associated with the completion of trophoblast invasion and placental growth. This study assessed the effect of malaria infection on the levels of IL-17, IL-10, and TGF-β in the plasma of pregnant mice with malaria. Methods: Seventeen pregnant BALB/C mice were divided into two groups: mice infected with Plasmodium berghei (treatment group) and uninfected mice (control group). The mice were sacrificed on day 18 post-mating. Parasitemia was measured by Giemsa staining. The levels of IL-17, IL-10, and TGF-β were measured by ELISA. Results: Using independent t test, the IL-17 levels in the treatment group were higher than those in the control group (P = 0.040). The IL-10 levels in the treatment group were lower than those in the control group (P = 0.00). There was no significant difference in the TGF-β levels (P = 0.055) between two groups. However, using SEM analysis the degree of parasitemia decreased the plasma TGF-β levels (tcount = 5.148; ≥ ttable= 1.96). SEM analysis showed that a high degree of parasitemia increased the IL-17 levels and decreased the IL-10 and TGF-β levels. Conclusion: Malaria infection during pregnancy interferes with the systemic balance by increasing the IL-17 levels and decreasing the IL-10 and TGF-β levels. [ABSTRACT FROM AUTHOR]
- Published
- 2015