1. Cyanobacteria as test organisms and biosorbents.
- Author
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Fokina, A., Ogorodnikova, S., Domracheva, L., Lyalina, E., Gornostaeva, E., Ashikhmina, T., and Kondakova, L.
- Subjects
CYANOBACTERIA ,ABSORPTION ,BIOLOGICAL assay ,LABORATORY organisms ,BIOFILMS - Abstract
Bioassay and biosorption potentials of different groups of cyanobacteria ( CB)- Nostoc linckia (Roth.) Born. et Flah. No. 271, natural biofilms dominated by CB of Phormidium genus, and biofilms dominated by Nostoc commune (Vauch. Elenk)-were estimated. The physiological-biochemical response of CB to the influence of copper sulfate (II) (catalase activity with a gasometric method and dehydrogenase activity, lipid peroxidation, and chlorophyll ɑ and pheophytin contents with a spectrophotometric method) was studied; metal bioaccumulation was determined with a stripping voltammetry method. It was found that the communities dominated by Phormidium genus (CB biomass 0.2 g/dm) removed copper compounds from the solutions with Cu ion concentration of 20 mg/dm almost completely (by 99%); communities dominated by CB N. commune, by 87%; and pure culture of N. linckia, by 50%. Dehydrogenase and catalase activities and the intensity of bioluminescence proved to be sensitive indicators of the response of CB to Cu ions. The impact of Cu ions (20 mg/dm) on a biofilm dominated by CB of Phormidium genus resulted in the fivefold decrease of catalase activity during 24 h; dehydrogenase activity decreased by nearly 357 times. The bioluminescence intensity during 24 h decreased by 1.3-100 times under the impact of Cu (2 mg/dm) and by 8.6-200 times in variants with a higher concentration of Cu (20 mg/dm). This regularity can be used as a test function in bioassay. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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