1. Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS.
- Author
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Rudolph G, Schilbach-Stückle K, Handgretinger R, Kaiser P, and Hameister H
- Subjects
- Blotting, Northern, Blotting, Southern, Chromosomes, Human, Pair 17, DNA, Neoplasm, Female, Gene Amplification, Genes, myc, Humans, Infant, Karyotyping, Trisomy, Abdominal Neoplasms genetics, Neuroblastoma genetics, Tumor Cells, Cultured
- Abstract
A new human neuroblastoma cell line (LS) that originated from an abdominal tumor of a 16-month-old girl is presented; it was classified, according to Evans, as being stage III. Morphological (dense-core particles) and biochemical characteristics (dopamine-beta-hydroxylase, acetylcholinesterase, neuron-specific-enolase) confirmed the diagnosis. In addition to a slightly variable modal chromosome number of 48 or 49 (because of marker-chromosomes and autosomal trisomies), cytogenetic analysis revealed two constantly appearing chromosomes with homogeneously stained regions (HSR's). The karyotype remained constant over 50 passages in vitro [49,XX, -12, +der5, + 17, + mar1, + mar2]. Double minutes were a rare phenomenon and appeared only in a few metaphases. In situ hybridization showed that some of the HSR's consisted of amplified N-myc copies. The distribution of the N-myc copies according to in situ hybridization signals along the HSR's was compared with the data of Southern and Northern blotting analyses.
- Published
- 1991
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