1. Decreased RGS9 protein level in the striatum of rodents undergoing MPTP or 6-OHDA neurotoxicity
- Author
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Yin, Lin-Lin, Cao, Yan, and Xie, Ke-Qiang
- Subjects
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NEOSTRIATUM , *LABORATORY rodents , *NEUROTOXICOLOGY , *SYMPTOMATIC Parkinson's disease , *CELLULAR signal transduction , *GENE expression , *TYROSINE , *MOLECULAR biology , *METHYLPHENYLTETRAHYDROPYRIDINE - Abstract
Abstract: Western blot has been used to study the time-course effect of the two most popular parkinsonian neurotoxins, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, i.p.) and 6-hydroxydopamine (6-OHDA, intra-substantia nigra), on the expression of several regulators of G-protein signaling (RGS2, 4 and 9) in striatum in rodents. During the few days after MPTP challenge, there was a decline (as expected) in tyrosine hydroxylase expression in the mouse striatum that was accompanied by a decline in RGS9 protein; the latter was specific and did not extend to RGS2 or RGS4 which were resistant to the MPTP challenge. Much the same pattern was observed in rats after 6-OHDA challenge, again, specific to RGS9, although the effect takes a few weeks, rather than a few days, to develop. These results may be helpful for the understanding of molecular mechanism underlying Parkinson''s disease (PD) and RGS9 might involve in the striatal function associated with PD. [Copyright &y& Elsevier]
- Published
- 2010
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