1. Apyrase-Mediated Amplification of Secretory IgA Promotes Intestinal Homeostasis
- Author
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Perruzza, Lisa, Strati, Francesco, Raneri, Matteo, Li, Hai, Gargari, Giorgio, Rezzonico-Jost, Tanja, Palatella, Martina, Kwee, Ivo, Morone, Diego, Seehusen, Frauke, Sonego, Paolo, Donati, Claudio, Franceschi, Pietro, Macpherson, Andrew J, Guglielmetti, Simone, Greiff, Victor, Grassi, Fabio, Perruzza, L, Strati, F, Raneri, M, Li, H, Gargari, G, Rezzonico-Jost, T, Palatella, M, Kwee, I, Morone, D, Seehusen, F, Sonego, P, Donati, C, Franceschi, P, Macpherson, A, Guglielmetti, S, Greiff, V, Grassi, F, University of Zurich, and Grassi, Fabio
- Subjects
10184 Institute of Veterinary Pathology ,Genetics and Molecular Biology ,610 Medicine & health ,Mice ,Peyer's Patches ,Adenosine Triphosphate ,Extracellular ATP ,1300 General Biochemistry, Genetics and Molecular Biology ,Settore MED/04 - PATOLOGIA GENERALE ,Animals ,Homeostasis ,Intestinal Mucosa ,T follicular helper cell ,Bacteria ,dysbiosi ,Microbiota ,Purinergic signaling ,Apyrase ,CP: Immunology ,Intestines ,Secretory IgA ,General Biochemistry ,Immunoglobulin A, Secretory ,570 Life sciences ,biology ,Dysbiosis ,610 Medizin und Gesundheit - Abstract
Secretory immunoglobulin A (SIgA) interaction with commensal bacteria conditions microbiota composition and function. However, mechanisms regulating reciprocal control of microbiota and SIgA are not defined. Bacteria-derived adenosine triphosphate (ATP) limits T follicular helper (Tfh) cells in the Peyer's patches (PPs) via P2X7 receptor (P2X7R) and thereby SIgA generation. Here we show that hydrolysis of extracellular ATP (eATP) by apyrase results in amplification of the SIgA repertoire. The enhanced breadth of SIgA in mice colonized with apyrase-releasing Escherichia coli influences topographical distribution of bacteria and expression of genes involved in metabolic versus immune functions in the intestinal epithelium. SIgA-mediated conditioning of bacteria and enterocyte function is reflected by differences in nutrient absorption in mice colonized with apyrase-expressing bacteria. Apyrase-induced SIgA improves intestinal homeostasis and attenuates barrier impairment and susceptibility to infection by enteric pathogens in antibiotic-induced dysbiosis. Therefore, amplification of SIgA by apyrase can be leveraged to restore intestinal fitness in dysbiotic conditions.
- Published
- 2022
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