1. Bioreactor design for the mechanical stimulation by compression of 3D cell cultures
- Author
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Micheler Carina M., Geck Paulina A., Charitou Fiona, Leix René, Foehr Peter, Lang Jan J., Wilhelm Nikolas J., Tuebel Jutta L., and Burgkart Rainer H. H.
- Subjects
bioreactor ,compression ,tissue engineering ,3d cell culture ,bone cell culture ,osteoblasts ,Medicine - Abstract
Bioreactors with a controlled physiological environment are being developed to study various cell processes. The influences of mechanostimulation on bone cell cultures can be investigated using a compression bioreactor. The developed bioreactor system applies a cyclic compression force to the specimen via an eccentrically mounted push rod. The compression force is monitored by a force sensor to detect changes in the material properties of the specimen. Depending on the piston setting, a stroke of 0.28 - 2.50 mm can be applied to the specimen. The bioreactor system was tested with a trial run of 18 days. A sample was continuously stimulated with a loading frequency of 2 Hz and a stroke of 1.50 mm. The sterility in the cell chamber as well as the functionality of the realised bioreactor stimulation system could be successfully confirmed
- Published
- 2021
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