1. Chondroprotective properties of human-enriched serum following polyphenol extract absorption: results from an exploratory clinical trial
- Author
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Ruth Hornedo-Ortega, Stéphanie Krisa, Nelly Urban, Nathalie Meunier, Elsa Mevel, Henri Granel, Juliette Berger, Laurent Beck, Tristan Richard, Jérôme Guicheux, Yohann Wittrant, Line Boutin-Wittrant, Stéphane Descamps, Josep Valls, Claire Vinatier, Adeline Blot, Fabien Wauquier, Wittrant, Yohann, Unité de Nutrition Humaine (UNH), Clermont Université-Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Recherche Agronomique (INRA), Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Unité de Recherche Oenologie [Villenave d'Ornon], Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Modulation Artificielle des Genes Eucaryotes, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Union Grap'Sud, Ortho-Traumatologie, Chirurgie plastique et reconstructive, Centre hospitalier universitaire Clermont Ferrand, Université de Nantes - UFR Odontologie, Université de Nantes (UN), CRNH Auvergne, Centre Hospitalier Universitaire de Clermont-Ferrand, Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Jehan, Frederic, Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Role of intra-Clonal Heterogeneity and Leukemic environment in ThErapy Resistance of chronic leukemias (CHELTER), Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Service d’Orthopédie Traumatologie [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Centre de Recherche en Nutrition Humaine Auvergne [CHU Clermont-Ferrand] (CRNH A), Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Clermont-Ferrand, and Université de Nantes - UFR Odontologie (UFR Odonto)
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Male ,0301 basic medicine ,[SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology ,Interleukin-1beta ,Anti-Inflammatory Agents ,Osteoarthritis ,Pharmacology ,chemistry.chemical_compound ,osteoarthritis (OA) ,cell biology ,Ingestion ,Cells, Cultured ,ComputingMilieux_MISCELLANEOUS ,[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system ,Nutrition and Dietetics ,[SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology ,Chemistry ,[SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology ,NF-kappa B ,[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology ,Phenylethyl Alcohol ,procyanidins ,Healthy Volunteers ,3. Good health ,medicine.anatomical_structure ,[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system ,Alimentation et Nutrition ,hydroxytyrosol ,Adult ,[SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,Article ,Chondrocyte ,Young Adult ,03 medical and health sciences ,Chondrocytes ,In vivo ,medicine ,[SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,Humans ,Food and Nutrition ,Proanthocyanidins ,micronutrients ,clinical trials ,030109 nutrition & dietetics ,Grape Seed Extract ,Plant Extracts ,Polyphenols ,medicine.disease ,In vitro ,030104 developmental biology ,Absorption, Physicochemical ,Polyphenol ,Hydroxytyrosol ,Ex vivo ,Food Science - Abstract
Polyphenols are widely acknowledged for their health benefits, especially for the prevention of inflammatory and age-related diseases. We previously demonstrated that hydroxytyrosol (HT) and procyanidins (PCy), alone or in combination, drive preventive anti-osteoathritic effects in vivo. However, the lack of sufficient clinical evidences on the relationship between dietary phytochemicals and osteoarthritis remains. In this light, we investigated in humans the potential osteoarticular benefit of a grapeseed and olive extract (OPCO) characterized for its hydroxytyrosol (HT) and procyanidins (PCy) content. We first validated, in vitro, the anti-inflammatory and chondroprotective properties of the extract on primary cultured human articular chondrocytes stimulated by interleukin-1 beta (IL-1 &beta, ). The sparing effect involved a molecular mechanism dependent on the nuclear transcription factor-kappa B (NF-&kappa, B) pathway. To confirm the clinical relevance of such a nutritional strategy, we designed an innovative clinical approach taking into account the metabolites that are formed during the digestion process and that appear in circulation after the ingestion of the OPCO extract. Blood samples from volunteers were collected following ingestion, absorption, and metabolization of the extract and then were processed and applied on human primary chondrocyte cultures. This original ex vivo methodology confirmed at a clinical level the chondroprotective properties previously observed in vitro and in vivo.
- Published
- 2019