1. Genetic Characterization of Toxoplasma gondii Isolates and Toxoplasmosis Seroprevalence in Stray Cats of Izmir, Turkey
- Author
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Aysu Değirmenci Döşkaya, Çağdaş Çetinkaya, Cemal Ün, Esra Atalay, Sabire Karaçali, Mert Döşkaya, Saygun Ürgen, Yüksel Gürüz, Hüseyin Can, Ayşe Caner, Daniel Ajzenberg, Sultan Gülçe İz, H. Gökhan Özdemir, Marie-Laure Dardé, Ege Üniversitesi, Neuroépidémiologie Tropicale (NET), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Limoges (UNILIM), Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC) (CNR Toxoplasmose-Toxoplasma BRC), CHU Limoges, and Grelier, Elisabeth
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Veterinary medicine ,lcsh:Medicine ,Cat Diseases ,law.invention ,Mice ,law ,Seroepidemiologic Studies ,Genotype ,Medicine and Health Sciences ,lcsh:Science ,Polymerase chain reaction ,education.field_of_study ,Vaccines ,Multidisciplinary ,CATS ,biology ,Vaccination and Immunization ,3. Good health ,ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS ,Transmission (mechanics) ,Infectious Diseases ,Oncology ,Female ,InformationSystems_MISCELLANEOUS ,Toxoplasma ,Cancer Prevention ,Toxoplasmosis ,Research Article ,Neglected Tropical Diseases ,Infectious Disease Control ,Population ,Immunology ,Cancer Vaccines ,Microbiology ,Virology ,Vaccine Development ,parasitic diseases ,medicine ,Parasitic Diseases ,Seroprevalence ,Animals ,education ,Protozoan Infections ,ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS ,lcsh:R ,Immunity ,Toxoplasma gondii ,Biology and Life Sciences ,Viral Vaccines ,medicine.disease ,biology.organism_classification ,Tropical Diseases ,ComputingMethodologies_PATTERNRECOGNITION ,Toxoplasmosis, Animal ,[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie ,Cats ,Clinical Immunology ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,lcsh:Q ,Microsatellite Repeats - Abstract
WOS: 000340879300061, PubMed ID: 25127360, Currently, some Toxoplasma gondii genotypes are being associated with serious clinical presentations. A recent report showing the Africa 1 genotype in two local congenital toxoplasmosis cases acquired in Turkey formed the basis of this study because atypical Africa 1 genotype is most frequently detected in animals and patients from sub-Saharan Africa. Since stray cats are considered as the linkage between wild life and urban life in T. gondii transmission, the present study aimed to isolate and characterize T. gondii strains circulating in stray cats of Izmir (Western Turkey). A secondary objective was to determine toxoplasmosis seroprevalence in this cat population. Tissues obtained from 100 deceased stray cats were bioassayed and isolated strains were genotyped using 15 microsatellite markers. In addition, toxoplasmosis seroprevalence was analyzed in 1121 cat sera collected from several large veterinary clinics in Izmir. Among the 22 isolates, 19 were Type II (86.3%), two were Type III (9%) and one was Africa 1 genotype (4.5%). The overall seropositivity rates in cats were 42-48% and 33.4-34.4% according to IFA and ELISA, respectively. Seroprevalence in deceased cats was significantly higher than in healthy cats (P = 0.0033). Finding both the major clonal Type II lineage together with the Type III lineage also found in Middle East, and an atypical genotype, Africa 1 appears consistent with the specific geographic location of Turkey between three continents and raises the possibility of transportation of these strains between continents through trade routes or long distance migratory birds. In addition, the first large study of toxoplasma seroprevalence in a stray cat population was also reported. The relatively high seropositivity rates and the variety of T. gondii genotypes confirm the local stray cat population as a risk factor for human toxoplasmosis in Izmir., Scientific Research Projects Branch Directorate of Ege University, TurkeyEge University [2010-TIP-091, 2011-TIP-034], This study was supported by the grants given by the Scientific Research Projects Branch Directorate of Ege University, Turkey (Grant No: 2010-TIP-091 and 2011-TIP-034) to Y.G. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Published
- 2014