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This paper's objective is to investigate the display fatigue quantitatively according to watching 2D and 3D videos on the display. Though it has been known commonly that display fatigue by 3D is greater than one by 2D, there have been few researches with an aim to measure display fatigue scientifically. Flicker fusion frequency and cybersickness degree were measured before/after sixteen subjects(Male 8, Female 8) watch the 2D, 3D images. Two dependent variables affected by 2D and 3D videos were analyzed and compared statistically based on scientific evidences and researches. 3D showed a value 4 times as much as 2D in cybersickness increase rate ,and the reduction rate of 3D was 2 times as much as that of 2D. [ABSTRACT FROM AUTHOR]