1. Gestational Perfluoroalkyl Substance Exposure and DNA Methylation at Birth and 12 Years of Age: A Longitudinal Epigenome-Wide Association Study
- Author
-
Yun Liu, Melissa N. Eliot, George D. Papandonatos, Karl T. Kelsey, Ruby Fore, Scott Langevin, Jessie Buckley, Aimin Chen, Bruce P. Lanphear, Kim M. Cecil, Kimberly Yolton, Marie-France Hivert, Sharon K. Sagiv, Andrea A. Baccarelli, Emily Oken, and Joseph M. Braun
- Subjects
Adolescent ,Health, Toxicology and Mutagenesis ,Toxicology ,Medical and Health Sciences ,Epigenome ,Clinical Research ,Pregnancy ,Genetics ,2.2 Factors relating to the physical environment ,2.1 Biological and endogenous factors ,Humans ,Longitudinal Studies ,Aetiology ,Child ,Pediatric ,Fluorocarbons ,Infant, Newborn ,Public Health, Environmental and Occupational Health ,Infant ,DNA Methylation ,Newborn ,Good Health and Well Being ,Alkanesulfonic Acids ,Environmental Pollutants ,Female ,Environmental Sciences - Abstract
BackgroundDNA methylation alterations may underlie associations between gestational perfluoroalkyl substances (PFAS) exposure and later-life health outcomes. To the best of our knowledge, no longitudinal studies have examined the associations between gestational PFAS and DNA methylation.ObjectivesWe examined associations of gestational PFAS exposure with longitudinal DNA methylation measures at birth and in adolescence using the Health Outcomes and Measures of the Environment (HOME) Study (2003-2006; Cincinnati, Ohio).MethodsWe quantified serum concentrations of perfluorooctanoate (PFOA), perfluorooctane sulfonate (PFOS), perfluorononanoate (PFNA), and perfluorohexane sulfonate (PFHxS) in mothers during pregnancy. We measured DNA methylation in cord blood (n=266) and peripheral leukocytes at 12 years of age (n=160) using the Illumina HumanMethylation EPIC BeadChip. We analyzed associations between log2-transformed PFAS concentrations and repeated DNA methylation measures using linear regression with generalized estimating equations. We included interaction terms between children's age and gestational PFAS. We performed Gene Ontology enrichment analysis to identify molecular pathways. We used Project Viva (1999-2002; Boston, Massachusetts) to replicate significant associations.ResultsAfter adjusting for covariates, 435 cytosine-guanine dinucleotide (CpG) sites were associated with PFAS (false discovery rate, q
- Published
- 2022
- Full Text
- View/download PDF