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13 results on '"Sébastien Artigaud"'

1. Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale

Author

Jean Armengaud, Grégory Charrier, Elodie Borcier, Justine Receveur, Baghdad Ouddane, Mamadou Diop, Jean Laroche, Jean-Charles Gaillard, Rachid Amara, Jérôme Couteau, Sébastien Artigaud, Vianney Pichereau, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire de Biochimie des Systèmes Perturbés (LBSP), TOXEM, Laboratoire de Spectrochimie Infrarouge et Raman - UMR 8516 (LASIR), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en économie et management (CREM), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Université de Lille, Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Innovations technologiques pour la Détection et le Diagnostic (LI2D), Service de Pharmacologie et Immunoanalyse (SPI), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Médicaments et Technologies pour la Santé (MTS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre), Cedre, Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), Région Bretagne, Seine Aval 6 –HQFish, Agence Française pour la Biodiversité –POPEST, Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Service de Pharmacologie et d'Immunoanalyse (SPI), Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille Institut (CLIL), and Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS)

Subjects
Environmental Engineering, 010504 meteorology & atmospheric sciences, Proteome, Zoology, Flounder, 010501 environmental sciences, Ecotoxicology, 01 natural sciences, Estuary water quality, Condition index, Caging, Fish physiology, [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry, Water Quality, Shotgun proteomics, Environmental Chemistry, Animals, 14. Life underwater, EUROPEAN FLOUNDER, Waste Management and Disposal, 0105 earth and related environmental sciences, Pollutant, geography, geography.geographical_feature_category, biology, ACL, Estuary, biology.organism_classification, Suess, Caging Stress, Pollution, Platichthys, 13. Climate action, Water quality, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Estuaries, Water Pollutants, Chemical, Environmental Monitoring
Abstract

International audience; Estuaries are important areas highly vulnerable to anthropogenic pollutions. Therefore, the assessment of estuarine water quality is a major ecological issue. In this study, we sampled juveniles of the European flounder in the “pristine” Canche estuary, and caged them in Canche and in two polluted sites of the Seine estuary, Rouen and Fosse Nord. After one month, the metal and organic pollutants in these sites were assessed, and we evaluated several phenotypic indicators (condition index, RNA/DNA ratios and genotoxicity), and extracted the proteins in fish livers for analysis using a shotgun proteomics approach.The results showed strong modifications in the fish caged in both sites of the Seine estuary, as compared to those caged in Canche. In particular, many proteins involved in phase I and phase II detoxification reactions were accumulated in the liver of fish caged in the site showing the highest pollution, Rouen. In addition, we observed a general disruption of metabolism, in particular an increase in lipid synthesis and carbohydrate degradation in Rouen, and a decrease in the abundance of proteins associated to translational activity in Fosse Nord. At both sites, several stress proteins were decreased.The proteomic impact of the encagement by itself was also evaluated, by comparing the liver proteome of fish caged in Canche to that of fish stayed in natura during the same time. The results showed proteomic signatures of exposure to stressful conditions (particularly heat stress), most probably related to the micro-habitat in which the cages were placed. In conclusion, the caging technique is of great interest for ecotoxicological assessment of estuarine waters, but should consider that the results are representative of the micro-habitat around the cages, which does not necessarily represent the overall heterogeneity of the estuarine environment.

Published
2019

2. A proteomic study of resistance to brown ring disease in the manila clam, Ruditapes philippinarum

Author

Christine Paillard, Luca Bargelloni, Sébastien Artigaud, Vianney Pichereau, Morgan Smits, Benoit Bernay, Universita degli Studi di Padova, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Interactions Cellules Organismes Environnement (ICORE), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), LabexMER [ANR-10-LABX-19], ISblue [ANR-17-EURE-0015], EU Horizon2020 project VIVALDI [678589], ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017), ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016), University of Padova, Department of Comparative Biomedicine and Food Science, Department of Comparative Biomedicine and Food Science, The authors would like to acknowledge the 'investment for the future' programs LabexMER (ANR-10-LABX-19) and ISblue (ANR-17-EURE-0015) for funding international joint PhD agreements and proteomic analyses, the EU Horizon2020 project VIVALDI (grant agreement N°678589) for providing funding and biological material., Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Padova = University of Padua (Unipd), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, and Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)

Subjects
0301 basic medicine, Proteomics, Resistance, Defence mechanisms, Manila clam, Diseases, Aquaculture, Transcriptome, Tandem Mass Spectrometry, Ruditapes philippinarum, Homeostasis, Pathogen, ComputingMilieux_MISCELLANEOUS, Disease Resistance, Vibrio tapetis, Chromatography, Liquid, 0303 health sciences, biology, Bivalve, 04 agricultural and veterinary sciences, General Medicine, Phenotype, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Brown ring disease, Host-Pathogen Interactions, [SDE]Environmental Sciences, Oxidation-Reduction, animal structures, Immune response, Animals, Bivalvia, Chromatography, Liquid, Vibrio, Vibrio Infections, Ruditapes, Aquatic Science, Microbiology, 03 medical and health sciences, Environmental Chemistry, 14. Life underwater, 030304 developmental biology, business.industry, Acl, Proteomic, biology.organism_classification, 030104 developmental biology, Infectious disease (medical specialty), 040102 fisheries, 0401 agriculture, forestry, and fisheries, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business, Bacteria
Abstract

International audience; Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by Brown Ring disease (BRD), an infection induced by the bacterium Vibrio tapetis. In this study, juvenile Manila clams were subjected to a 28-day controlled challenge with Vibrio tapetis, and visual and molecular diagnoses were carried out to distinguish two extreme phenotypes within the experimental clams: uninfected ("RES", resistant) and infected ("DIS", diseased) post-challenge. Total protein extractions were carried out for resistant and diseased clams, and proteins were identified using LC-MS/MS. Protein sequences were matched against a reference transcriptome of the Manila clam, and protein intensities based on label-free quantification were compared to reveal 49 significantly accumulated proteins in resistant and diseased clams. Proteins with known roles in pathogen recognition, lysosome trafficking, and various aspects of the energy metabolism were more abundant in diseased clams, whereas those with roles in redox homeostasis and protein recycling were more abundant in resistant clams. Overall, the comparison of the proteomic profiles of resistant and diseased clams after a month-long controlled challenge to induce the onset of Brown Ring disease suggests that redox homeostasis and maintenance of protein structure by chaperone proteins may play important and interrelated roles in resistance to infection by Vibrio tapetis in the Manila clam.

Published
2019

3. Metal subcellular partitioning determines excretion pathways and sensitivity to cadmium toxicity in two marine fish species

Author

Sébastien Artigaud, Marie-Laure Rouget, Jean Raffray, Stéphane Le Floch, Gaël Le Croizier, Virginie Penicaud, Raymond Laë, Camille Lacroix, Luis Tito de Morais, Jean-Marie Munaron, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre), Cedre, Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), ANR-11-CEPL-0005,ÉPURE,Éléments trace métalliques Perturbations climatiques Upwelling et REssources(2011), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Antioxidant, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Solea senegalensis, 0208 environmental biotechnology, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Cell membrane, Cadmium, biology, Sub-cellular fractionation, Chemistry, General Medicine, Pollution, medicine.anatomical_structure, Biochemistry, Liver, EPURE, Metals, Inactivation, Metabolic, Flatfishes, Dicentrarchus, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Depuration, Environmental Engineering, Elimination, chemistry.chemical_element, Excretion, Species Specificity, Organelle, medicine, Environmental Chemistry, Animals, Dicentrarchus labrax, 14. Life underwater, Sea bass, 0105 earth and related environmental sciences, ACL, Public Health, Environmental and Occupational Health, General Chemistry, Essential element distribution, biology.organism_classification, 020801 environmental engineering, Bass, Metallothionein, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Homeostasis, Water Pollutants, Chemical
Abstract

International audience; Subcellular cadmium (Cd) partitioning was investigated in the liver of two marine fish species, the European sea bass Dicentrarchus labrax and the Senegalese sole Solea senegalensis, dietary exposed to an environmentally realistic Cd dose for two months followed by a two-month depuration. Cd exposure did not modify Cd cellular partitioning for either species, refuting the spillover hypothesis. Both species contained most of the Cd in the detoxifying fraction but displayed different handling strategies. Cd was largely bound to heat stable proteins (HSP) including metallothioneins (MT) in sea bass while Cd was more linked to metal rich granules (MRG) in sole. Whole liver concentrations and subcellular partitioning were also determined for essential elements. The greatest impairment of essential metal homeostasis due to Cd exposure was found in sole. These elements followed the Cd partitioning pattern, suggesting that they are involved in antioxidant responses against Cd toxicity. Cd consumption diminished sole growth in terms of body weight, probably due to lipid storage impairment. During the depuration period, the two species showed contrasting partitioning patterns, implying different pathways for Cd elimination from the liver. In sea bass, MT-bound Cd would be excreted through bile or released into blood, crossing the cell membrane via a protein transporter. In sole, MRG-bound Cd would be sequestered by organelles before being released into the blood via vesicular exocytosis. These distinct strategies in cellular Cd handling in the liver might account for differential sensitivity to Cd toxicity and differential Cd excretion pathways between the two marine fish species.

Published
2018

4. Significance of metallothioneins in differential cadmium accumulation kinetics between two marine fish species

Author

Nicolas Le Bayon, Sébastien Artigaud, Virginie Penicaud, Gaël Le Croizier, Stéphane Le Floch, Julien Autier, Valérie Coquillé, Marie-Laure Rouget, Luis Tito de Morais, Raymond Laë, Jean Raffray, Camille Lacroix, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre), Cedre, Institut de Recherche pour le Développement (IRD), Université de Brest (UBO), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), ANR-11-CEPL-0005,ÉPURE,Éléments trace métalliques Perturbations climatiques Upwelling et REssources(2011), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne)

Subjects
0106 biological sciences, Health, Toxicology and Mutagenesis, Solea senegalensis, 010501 environmental sciences, Toxicology, 01 natural sciences, Metallothionein, oxidative stress, heavy metals, bass dicentrarchus-labrax, Cadmium, biology, Metal, Muscles, trace-elements, western indian-ocean, General Medicine, Chronic dietary exposure, Pollution, cd, Liver, Metals, Bioaccumulation, Flatfishes, Trace element, Dicentrarchus, Kinetics, Dietary Cadmium, chemistry.chemical_element, Zoology, Excretion, Animals, Dicentrarchus labrax, 14. Life underwater, Sea bass, 0105 earth and related environmental sciences, 010604 marine biology & hydrobiology, ACL, biology.organism_classification, rainbow-trout, chemistry, 13. Climate action, atomic-absorption-spectrometry, Bass, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Water Pollutants, Chemical, trout oncorhynchus-mykiss
Abstract

International audience; Impacted marine environments lead to metal accumulation in edible marine fish, ultimately impairing human health. Nevertheless, metal accumulation is highly variable among marine fish species. In addition to ecological features, differences in bioaccumulation can be attributed to species-related physiological processes, which were investigated in two marine fish present in the Canary Current Large Marine Ecosystem (CCLME), where natural and anthropogenic metal exposure occurs. The European sea bass Dicentrarchus labrax and Senegalese sole Solea senegalensis were exposed for two months to two environmentally realistic dietary cadmium (Cd) doses before a depuration period. Organotropism (i.e., Cd repartition between organs) was studied in two storage compartments (the liver and muscle) and in an excretion vector (bile). To better understand the importance of physiological factors, the significance of hepatic metallothionein (MT) concentrations in accumulation and elimination kinetics in the two species was explored. Accumulation was faster in the sea bass muscle and liver, as inferred by earlier Cd increase and a higher accumulation rate. The elimination efficiency was also higher in the sea bass liver compared to sole, as highlighted by greater biliary excretion. In the liver, no induction of MT synthesis was attributed to metal exposure, challenging the relevance of using MT concentration as a biomarker of metal contamination. However, the basal MT pools were always greater in the liver of sea bass than in sole. This species-specific characteristic might have enhanced Cd biliary elimination and relocation to other organs such as muscle through the formation of more Cd/MT complexes. Thus, MT basal concentrations seem to play a key role in the variability observed in terms of metal concentrations in marine fish species.

Published
2018

5. New insights into the seasonal feeding ecology of Pecten maximus using pigments, fatty acids and sterols analyses

Author

Anne Donval, Beatriz Beker, Philippe Soudant, Aude Leynaert, Sébastien Artigaud, Øivind Strand, Fabienne Le Grand, Fred Jean, Jonathan Flye-Sainte-Marie, Arnab Chatterjee, Tore Strohmeier, Romain Lavaud, Fisheries and Oceans Canada (DFO), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institute of Marine Research [Bergen] (IMR), University of Bergen (UiB), ANR-09-BLAN-0335,CHIVAS,Chimie des valves de la coquille Saint-Jacques européenne, archives multiproxies de la dynamique phytoplanctonique(2009), ANR-10-STRA-0010,COMANCHE,Interactions écosystémiques et impacts anthropiques dans les populations de Coquilles St-Jacques de la Manche(2010), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Pigments, Zoology, Chlorophyceae, Aquatic Science, 010603 evolutionary biology, 01 natural sciences, chemistry.chemical_compound, Water column, Trophic ecology, Phytoplankton, Pecten maximus, 14. Life underwater, Food sources, Fatty acids, Ecology, Evolution, Behavior and Systematics, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography, Ecology, biology, 010604 marine biology & hydrobiology, ACL, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Peridinin, chemistry, Trophic marker, Scallop, Seawater, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Dinophyceae
Abstract

International audience; We combined the use of pigments, fatty acids and sterols as biomarkers of the seasonal variation in food sources of the great scallop Pecten maximus. From March to October 2011, on a biweekly to twice-weekly basis, scallops and seawater from the water column and the water-sediment interface were collected in the Bay of Brest (Brittany, France). Pigment compositions in the seawater and in the stomach and rectum content of the scallops were analyzed by HPLC. Fatty acids and sterols from digestive gland (DG) tissue were analyzed by gas chromatography. Potential relationships between the temporal proportion of each marker in the environment and in the digestive tract were tested using multivariate analysis. Proportions of diatoms and Dinophyceae biomarkers alternated in the digestive tract of P. maximus. This switch of feeding was confirmed by the polar lipid composition of the DG. Peridinin, characterizing Dinophyceae, occurred in high proportions in the digestive tract compared to the low ambient concentration, suggesting a selection of this microalgae group by the scallop. Chlorophyceae and green macroalgae tracers were found in low proportions, suggesting they were not actually ingested. Markers of Prymnesiophyceae were also observed at significant levels. Cyanobacteria tracers showed that this microalgae class was not ingested by the scallops during monitoring but may be of higher importance during winter. Switching from one food source to another as well as selectivity in feeding are discussed relative to the season.

Published
2018

6. Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae

Author

Benoit Bernay, Claire Hellio, Pierre Boudry, Valentin Foulon, Kada Boukerma, Philippe Elies, Caroline Fabioux, Manon Buscaglia, Fabienne Guérard, Bruno Petton, Sébastien Artigaud, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Interactions Cellules Organismes Environnement (ICORE), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Université de Brest (UBO), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, and Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)

Subjects
0301 basic medicine, Oyster, pacific oyster, Proteome, media_common.quotation_subject, Zoology, lcsh:Medicine, 02 engineering and technology, l-dopa, Article, 03 medical and health sciences, biology.animal, Animals, 14. Life underwater, Crassostrea, Metamorphosis, Ostrea edulis, Author Correction, lcsh:Science, media_common, Larva, Multidisciplinary, biology, ACL, lcsh:R, fungi, Metamorphosis, Biological, ostrea-edulis, Substrate (biology), Pacific oyster, 021001 nanoscience & nanotechnology, biology.organism_classification, tube feet, adhesion, 030104 developmental biology, in-situ, histochemistry, foot, pediveliger, star asterias-rubens, lcsh:Q, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Transcriptome, 0210 nano-technology, Tube feet
Abstract

Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement.

Published
2018

7. Evolution of the plasma proteome of divers before and after a single SCUBA dive

Author

Otto F. Barak, François Guerrero, Ryan L. Hoiland, Jacky Lautridou, Zeljko Dujic, Benoit Bernay, Sébastien Artigaud, Vianney Pichereau, Ingrid Eftedal, Andrew T. Lovering, Optimisation des régulations physiologiques (ORPHY (EA 4324)), Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Interactions Cellules Organismes Environnement (ICORE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), University of Novi Sad, University of British Columbia (UBC), University of Oregon [Eugene], Norwegian University of Science and Technology [Trondheim] (NTNU), Norwegian University of Science and Technology (NTNU), University of Split, European Project: 264816,EC:FP7:PEOPLE,FP7-PEOPLE-2010-ITN,PHYPODE(2011), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Adult, Male, Proteomics, Operations research, Proteome, SCUBA, Diving, Clinical Biochemistry, Decompression sickness, 03 medical and health sciences, Plasma, 0302 clinical medicine, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, 14. Life underwater, business.industry, ACL, Blood Proteins, medicine.disease, Data science, 030104 developmental biology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business, human activities, 030217 neurology & neurosurgery, Human
Abstract

Purpose: Decompression sickness (DCS) is a poorly understood and complex systemic disease caused by inadequate desaturation following a reduction of ambient pressure. A previous proteomic study of ours showed that DCS occurrence but not diving was associated with changes in the plasma proteome in rats, including a dramatic decrease of abundance of the tetrameric form of Transthyretin (TTR). The present study aims to assess the impact on the human blood proteome of a dive inducing significant decompression stress but without inducing DCS symptoms. Experimental design: Twelve healthy male divers were subjected to a single dive at a depth of 18m of sea water (msw) with a 47-min bottom time followed by a direct ascent to the surface at a rate of 9msw/min. Venous blood was collected before the dive as well as 30mn and 2h following the dive. The plasma proteomes from four individuals were then analyzed by using a two-dimensional electrophoresis-based proteomic strategy. Results: No protein spot showed a significantly changed abundance (fdr< 0.1) between the tested times. Conclusion: These results strengthen the hypothesis according to which significant changes of the plasma proteome measurable with two-dimensional electrophoresis may only occur along with DCS symptoms. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the authors' accepted and refereed manuscript to the article. Locked until 12 May 2018 due to copyright restrictions

Published
2017

8. In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement

Author

Valentin Foulon, Fabienne Guérard, Pierre Boudry, Claire Hellio, Sébastien Artigaud, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, adhesive, Candidate gene, Oyster, lcsh:Chemistry, Transcriptome, 0302 clinical medicine, Pacific oyster, lcsh:QH301-705.5, Spectroscopy, Genetics, Larva, General Medicine, proteomic analysis, Computer Science Applications, pediveliger larvae, catechol oxidase, sandcastle worm, histochemistry, 030220 oncology & carcinogenesis, Crassostrea, animal structures, growth, In silico, bioadhesive, panorama, Biology, binding matrix protein, Catalysis, Inorganic Chemistry, 03 medical and health sciences, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], biology.animal, 14. Life underwater, Physical and Theoretical Chemistry, Molecular Biology, Gene, phragmatopoma-californica, ACL, fungi, Organic Chemistry, biology.organism_classification, byssus, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, foot, Crassostrea gigas, transcriptome
Abstract

WOS:000459747700197; International audience; Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae.

Published
2019

9. Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas

Author

Hafida Essid, Charlotte Corporeau, Sébastien Artigaud, Vianney Pichereau, Ewan Harney, Pierrick Le Souchu, Flavia L. D. Nunes, Philippe Miner, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010)

Subjects
vitellogenin gene, 0106 biological sciences, 0301 basic medicine, Hot Temperature, Proteome, Acclimatization, ocean acidification, 01 natural sciences, Biochemistry, Global Warming, mytilus-galloprovincialis, chemistry.chemical_compound, oxidative stress, strongylocentrotus-purpuratus, seawater acidification, Carbon dioxide in Earth's atmosphere, Ecology, GAPDH, Ocean acidification, Pacific oyster, Hydrogen-Ion Concentration, Larva, Carbon dioxide, Crassostrea, ATP synthase, Biophysics, Superoxide dismutase, Biology, carbon-dioxide, larval development, 03 medical and health sciences, Animals, 14. Life underwater, embryonic-development, marine organisms, ACL, 010604 marine biology & hydrobiology, Global warming, fungi, endoplasmic-reticulum, anthropogenic co2, Marine invertebrates, biology.organism_classification, 030104 developmental biology, chemistry, 13. Climate action, Larval development, Crassostrea gigas, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

00000 ăWOS:000372685800014; International audience; Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40 h under either control (pH 8.1, 20 degrees C), acidified (pH 7.9, 20 degrees C), warm (pH 8.1, 22 degrees C) or warm acidified (pH 7.9, 22 degrees C) conditions. Larvae in acidified conditions were significantly smaller than in the control, but warm acidified conditions mitigated negative effects on size, and increased calcification. A proteomic approach employing two-dimensional electrophoresis (2-DE) was used to quantify proteins and relate their abundance to phenotypic traits. In total 12 differentially abundant spots were identified by nano-liquid chromatography-tandem mass spectrometry. These proteins had roles in metabolism, intra- and extra-cellular matrix formations, stress response, and as molecular chaperones. Seven spots responded to reduced pH, four to increased temperature, and six to acidification and warming. Reduced abundance of proteins such as ATP synthase and GAPDH, and increased abundance of superoxide dismutase, occurred when both pH and temperature changes were imposed, suggesting altered metabolism and enhanced oxidative stress. These results identify key proteins that may be involved in the acclimation of C gigas larvae to ocean acidification and warming.Significance: Increasing atmospheric CO2 raises sea surface temperatures and results in ocean acidification, two climatic variables known to impact marine organisms. Larvae of calcifying species may be particularly at risk to such changing environmental conditions. The Pacific oyster Crassostrea gigas is ecologically and commercially important, and understanding its ability to acclimate to climate change will help to predict how aquaculture of this species is likely to be impacted. Modest, yet realistic changes in pH and/or temperature may be more informative of how populations will respond to contemporary climate change. We showed that concurrent acidification and warming mitigates the negative effects of pH alone on size of larvae, but proteomic analysis reveals altered patterns of metabolism and an increase in oxidative stress suggesting non-additive effects of the interaction between pH and temperature on protein abundance. Thus, even small changes in climate may influence development, with potential consequences later in life.

Published
2015

10. Proteomic responses to hypoxia at different temperatures in the great scallop (Pecten maximus)

Author

Joëlle Richard, Camille Lacroix, Luca Bargelloni, Jonathan Flye-Sainte-Marie, Sébastien Artigaud, Vianney Pichereau, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Universita degli Studi di Padova, ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), and ANR-10-STRA-0010,COMANCHE,Interactions écosystémiques et impacts anthropiques dans les populations de Coquilles St-Jacques de la Manche(2010)

Subjects
Hyperthermia, Bivalves, Arginine, lcsh:Medicine, Marine Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, medicine, Pecten maximus, 14. Life underwater, Cytoskeleton, Hypoxia, Molecular Biology, 030304 developmental biology, 0303 health sciences, biology, Ecology, General Neuroscience, ACL, lcsh:R, 030302 biochemistry & molecular biology, Temperature, Proteomic, General Medicine, Metabolism, Hypoxia (medical), medicine.disease, biology.organism_classification, Biochemistry, Scallop, Aquaculture, Fisheries and Fish Science, medicine.symptom, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Non-model, General Agricultural and Biological Sciences, Anaerobic exercise, Environmental Sciences
Abstract

Hypoxia and hyperthermia are two connected consequences of the ongoing global change and constitute major threats for coastal marine organisms. In the present study, we used a proteomic approach to characterize the changes induced by hypoxia in the great scallop, Pecten maximus, subjected to three different temperatures (10 °C, 18 °C and 25 °C). We did not observe any significant change induced by hypoxia in animals acclimated at 10 °C. At 18 °C and 25 °C, 16 and 11 protein spots were differentially accumulated between normoxia and hypoxia, respectively. Moreover, biochemical data (octopine dehydrogenase activity and arginine assays) suggest that animals grown at 25 °C switched their metabolism towards anaerobic metabolism when exposed to both normoxia and hypoxia, suggesting that this temperature is out of the scallops’ optimal thermal window. The 11 proteins identified with high confidence by mass spectrometry are involved in protein modifications and signaling (e.g., CK2, TBK1), energy metabolism (e.g., ENO3) or cytoskeleton (GSN), giving insights into the thermal-dependent response of scallops to hypoxia.

Published
2014

11. Respiratory response to combined heat and hypoxia in the marine bivalves Pecten maximus and Mytilus spp

Author

Sébastien Artigaud, Vianney Pichereau, Camille Lacroix, Jonathan Flye-Sainte-Marie, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Aquatic Organisms, Bivalves, Hot Temperature, Physiology, Acclimatization, Oceans and Seas, [SDV]Life Sciences [q-bio], Intertidal zone, Intertidal ecology, Biochemistry, Animals, Pecten maximus, Ecosystem, 14. Life underwater, Hypoxia, Molecular Biology, Mytilus, Marine biology, Pecten, biology, Ecology, Respiration, ACL, Temperature, Hypoxia (environmental), biology.organism_classification, musculoskeletal system, Habitat, Respiratory Physiological Phenomena, Oxyregulation, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

International audience; Coastal ecosystems are increasingly disturbed by the increase of mean sea surface temperature and expansion of hypoxic areas. The objectives of the present work were to describe and compare the respiratory responses to combined heat and hypoxia in two bivalve species (Pecten maximus and Mytilus spp.) living in two contrasted coastal habitats (subtidal and intertidal, respectively). Results were consistent with the vertical zonation of both species. Mytilus spp. seemed to cope better with a temperature increase than P. maximus, which was found to be outside of its optimal thermal window at 25°C. Concerning respiratory responses to hypoxia at a given temperature, P. maximus displayed greater oxyregulation capacity that was maintained over a larger range of O2 levels, as compared to Mytilus spp. When acclimation temperatures increased, both species showed a decrease in their oxyregulation capacities alongside a reduction in aerobic performance, especially in P. maximus. The comparison between species suggests that subtidal species, such as P. maximus, might be more vulnerable to a combination of heat and hypoxia than intertidal species, such as Mytilus spp. Lastly, this study highlighted the utility of segmented linear models to estimate PcO2 and regulation percentages in marine organisms exposed to hypoxia

Published
2014

12. Proteomic-based comparison between populations of the Great Scallop, Pecten maximus

Author

Sébastien Artigaud, Massimo Milan, Vianney Pichereau, Romain Lavaud, Tore Strohmeier, Fred Jean, Julien Thébault, Øivind Strand, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institute of Marine Research [Bergen] (IMR), University of Bergen (UiB), Department of Comparative Biomedicine and Food Science, Universita degli Studi di Padova, ANR-09-BLAN-0335,CHIVAS,Chimie des valves de la coquille Saint-Jacques européenne, archives multiproxies de la dynamique phytoplanctonique(2009), ANR-10-STRA-0010,COMANCHE,Interactions écosystémiques et impacts anthropiques dans les populations de Coquilles St-Jacques de la Manche(2010), and ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010)

Subjects
0106 biological sciences, Proteomics, Proteome, Filamin, [SDV]Life Sciences [q-bio], Population, Biophysics, macromolecular substances, 01 natural sciences, Biochemistry, 03 medical and health sciences, Pecten maximus, Animals, 14. Life underwater, Population proteomics, Cytoskeleton, Mantle (mollusc), education, Non-model species, Actin, 030304 developmental biology, 0303 health sciences, education.field_of_study, Pecten, biology, Ecology, 010604 marine biology & hydrobiology, MALDI TOF/TOF, ACL, biology.organism_classification, Adaptation, Physiological, Evolutionary biology, Scallop, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

International audience; Comparing populations residing in contrasting environments is an efficient way to decipher how organisms modulate their physiology. Here we present the proteomic signatures of two populations in a non-model marine species, the great scallop Pecten maximus, living in the northern (Hordaland, Norway) and in the center (Brest, France) of this species' latitudinal distribution range. The results showed 38 protein spots significantly differentially accumulated in mantle tissues between the two populations. We could unambiguously identify 11 of the protein spots by Maldi TOF-TOF mass spectrometry. Eight proteins corresponded to different isoforms of actin, two were identified as filamin, another protein related to the cytoskeleton structure, and one was the protease elastase. Our results suggest that scallops from the two populations assayed may modulate their cytoskeleton structures through regulation of intracellular pools of actin and filamin isoforms to better adapt to their environment. BIOLOGICAL SIGNIFICANCE: Marine mollusks are non-model organisms that have been poorly studied at the proteomic level, and this article is the first studying the great scallop (P. maximus) at this level. Furthermore, it addresses population proteomics, a new promising field, especially in environmental sciences. This article is part of a Special Issue entitled: Proteomics of non-model organisms.

Published
2014

13. Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics

Author

Romain Lavaud, Sébastien Artigaud, Vianney Pichereau, Joëlle Richard, Melody S. Clark, Lloyd S. Peck, Fred Jean, Michael A. S. Thorne, Jonathan Flye-Sainte-Marie, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), British Antarctic Survey (BAS), Natural Environment Research Council (NERC), ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), and ANR-10-STRA-0010,COMANCHE,Interactions écosystémiques et impacts anthropiques dans les populations de Coquilles St-Jacques de la Manche(2010)

Subjects
Proteomics, DNA repair, Apoptosis, Biology, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Energy reserves, Genetics, Animals, Pecten maximus, 14. Life underwater, Heat shock, 030304 developmental biology, Marine biology, Pecten, 0303 health sciences, Ecology, Gene Expression Profiling, ACL, Transcription regulation, biology.organism_classification, Adaptation, Physiological, Cell biology, Gene expression profiling, Metabolism, Pecten (genus), Scallop, Adaptation, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Heat-Shock Response, 030217 neurology & neurosurgery, Research Article, Biotechnology
Abstract

Background The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results Individuals were maintained at three different temperatures (15, 21 and 25 °C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 °C, but suffered a reduction in condition at 25 °C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative annotation assigned to 177 transcripts. The proteomic approach identified 24 differentially expressed proteins, with nine identified by mass spectrometry. Network analysis of these results indicated a pivotal role for GAPDH and AP-1 signalling pathways. Data also suggested a remodelling of the cell structure, as revealed by the differential expression of genes involved in the cytoskeleton and cell membrane and a reduction in DNA repair. They also indicated the diversion of energetic metabolism towards the mobilization of lipid energy reserves to fuel the increased metabolic rate at the higher temperature. Conclusions This work provides preliminary insights into the response of P. maximus to chronic heat stress and provides a basis for future studies examining the tipping points and energetic trade-offs of scallop culture in warming oceans. Electronic supplementary material The online version of this article (doi:10.1186/s12864-015-2132-x) contains supplementary material, which is available to authorized users.

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