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Wines produced from grapes cultivated in cool climate areas are characterized by high levels of organic acids. One method to correct this is malolactic fermentation (MLF). The aim of this study was to determine the effectiveness of different strains of Oenococcus oeni bacteria (Viniflora CH11, Viniflora CH16, Viniflora CH35, Viniflora Oenos, SIHA LACT Oeno) during the biological acidity reduction process. Red wine from Rondo and Regent cultivars was obtained by ethanol fermentation of the pulp, at 20 °C for 14 days. The profile of organic acids was examined with a particular focus on changes in the content of l-malic and l-lactic acids. Additionally, the impact on profile and quantity of phenolic compounds and antioxidant capacity was measured. The results showed that MLF had a positive influence on content of organic acids through the reduction of l-malic acid content with a simultaneous increase of the amount of l-lactic acid. The best effect was obtained with the CH11 and CH35 bacterial strains. The biological acidity reduction process had no significant (P > 0.05) impact on phenolic content or antioxidant capacity. However, the wine making process (ethanol fermentation, maturation) contributed to the reduction of polyphenols and in consequence lower antioxidant capacity of the final tested wines. PRACTICAL APPLICATION: The present study provides useful information on the impact of different Oenococcus oeni bacterial strains on MLF in red wines, reduction of l-malic to l-lactic acid, and stability of phenolic compounds during MLF and the maturation period. Also, this article provides information about phenolic compounds and antioxidant capacity during malolactic fermentation and maturity of red wines made from hybrids of Vitis vinifera such as Rondo and Regent cultivars.

Sea buckthorn (Hippophae rhamnoides L.) berries have high biological value as a rich source of phenolic compounds, fatty acids and vitamins A, C, E. Due to the high organic acid content and sour taste, the fruits are rarely used in juice production. Therefore, the study aimed to determine the metabolic activity of Lactobacillus plantarum, Lactobacillus plantarum subsp. argentoratensis and Oenococcus oeni strains along with the dynamics of changes in organic acids, sugars, phenolic compounds, and antioxidant activity during 72-h fermentation of 100% sea buckthorn and mixed with apple (1:1) juices. The strongest malolactic conversion was in mixed juices (to 75.0%). The most efficient strains were L. plantarum DSM 10492, 20174 and 6872. L. plantarum strains caused an increase in flavonols and antioxidant activity of sea buckthorn-apple juices. The results can be used to select conditions and strains in industrial-scale fermentation, to produce novel sea buckthorn products and increase their consumption.

The study investigated 15 variants of wine differing in their must pre-treatment (maceration by enzymes: Siha Pectinase or SihazymUni) and fermentation by different yeast strains (S.cerevisiae as ICV - D-47; SIHA- Cryaroma; Challenge Aroma White; and S. bayanus as SIHA Active Yeast). The effects of experimental conditions on chemical composition (pH, acidity, sugar), content of phenolic (LC–PDA-QTOF/MS) and volatile compounds (GC-MS), antioxidant activity (ABTS and FRAP assay), color property (CIEL*a*b*), and ethanol content were measured. For all production processes, significant changes in basic parameters and in the amount of polyphenolic compounds and antioxidant activity in wine were observed as compared to must. The wines obtained with S. cerevisiae were characterized by higher polyphenol content, smaller reduction of antioxidant activity and the strongest aroma. Challenge Aroma White yeast strain particularly contributed to good quality of wine. Enzymatic pre-treatment did not significantly affect the studied parameters (expect pH and the content of phenolic acids).

The aim of this study was to investigate the influence of various Saccharomyces cerevisiae and Saccharomyces bayanus on the content of phenolic compounds (UPLC-PDA-FL), antioxidant activity (ABTS and FRAP), colour (CIEL*a*b*) and sensory attributes of chokeberry wine. The pH, content of sugar, polyphenols, total acidity, colour, and antioxidant activity were evaluated in chokeberry must, wine after fermentation, and wine after 10 months of storage at 4 and 25 °C. High contents of polyphenol compounds and antioxidant activity were obtained in chokeberry wine after storage at 4 °C. The colour and sensory attributes of these wines were intensively red, attractive and accepted by the consumer. The temperature during storage significantly influenced increase in the quality of the products. However, the different types of yeasts used to ferment chokeberry wine did not significantly affect the bioactive compounds, antioxidant activity or colour of chokeberry wine.

This study present the effects of different pre-treatment maceration techniques (microwave, thermo-maceration, and enzymatic treatment) on the content of phenolic compounds (by UPLC-PDA) and their redox potential by cyclic voltammetry (CV), antioxidant capacity (ABTS radical cation decolorization assay and FRAP as Ferric Reducing Ability of Plasma), and other basic chemical properties of red wine made from cv. Dornfelder. The pre-treatment maceration technique significantly (p 0.05) affected the content of total phenolics in Dornfelder must (freshly crushed grapes), with particularly high amounts in the must pre-treated by microwaves (4344.0 mg/100 mL), and the lowest in the must pre-treatment by thermo-maceration (2979.8 mg/100 mL). A positive correlation was found between the content of total phenolics and antioxidant activity (R = 0.69 and 0.52 by ABTS and FRAP assay, respectively) and individual groups of polyphenols (for ABTS assay with anthocyanins, flavonols, flavan-3-ols, respectively R = 0.60, 0.64 and 0.66, while for FRAP method only for anthocyanins R = 0.79). The highest antioxidant activity was also determined for the variant with microwave pre-treatment (must 3.31 mM and 3.05 mM Trolox/100 mL for ABTS and FRAP assay, respectively). Following the fermentation and maturation, the amount of polyphenols decreased. No thermal pre-treatment maceration methods gave lighter or redder must. After winemaking process all the samples were characterized by less red and more yellow shade.

The yeast strain and storage time is an important factor affecting the development of phytochemicals and sensory attributes in ciders. Therefore, the aim of this study was to determine the influence of yeast strains (Saccharomyces bayanus and Saccharomyces cerevisiae), &beta, cyclodextrin (BCD), and storage time on physicochemical parameters, contents of phenolic compounds (ultra-performance liquid chromatography with photodiode array detector coupled to quadrupole time-of-flight tandem mass spectrometry (UPLC&ndash, PDA&ndash, QToF-MS/MS)), antioxidative activity (free radical-scavenging ability (ABTS) and ferric reducing antioxidative power (FRAP) assay), and sensory attributes of new cider from the &ldquo, Bella Marii&rdquo, cultivar of red apple. The pH value, acidity, concentrations of alcohol, organic acids, and polyphenols, and the color and antioxidative properties were evaluated in red apple ciders immediately after fermentation and after three months of storage at 4 &deg, C. S. cerevisiae SIHAFERM Finesse Red with BCD and SIHAFERM Finesse Red yeast strain especially contributed to obtaining ciders with a high content of the tested compounds. The use of BCD during fermentation significantly influenced the protection of bioactive compounds, by as much as 18%. Storage time had an impact on concentrations of the tested components (mainly on the total flavan-3-ols and phenolic acids). Based on the achieved values of parameters analyzed in red apple ciders and results of the consumer acceptance test, it may be concluded that red apple offers vast potential for the production of ciders with a high content of polyphenolic compounds.

The aim of this study was to identify and compare phenolic acids, flavan-3-ols, flavonols, and anthocyanins in two the most popular interspecific hybrids of red grapes, Rondo and Regent, nowadays very popular in red wine production in Poland. The phenolic profiles of these hybrids have not yet been reported. Thirty-three phenolic compounds, including 2 flavan-3-ols, 3 phenolic acids, 5 flavonols, and 23 anthocyanins, were determined in the examined samples using the ultra-performance liquid chromatography photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS) method. Major differences were found in the phenolic profiles of investigated cultivars. The Regent hybrid exhibited the highest total phenolics content (27029.75 mg/kg dry matter) but Rondo was characterized by the highest concentration of anthocyanins (19342.36 mg/kg dm). The dominant fraction was anthocyanin compounds, especially acetylated > diglucosylated forms than glucosylated ones. This data represents valuable information that may be useful for oenological practices and to valorise these varieties as sources of bioactive compounds.

This study investigated the effects of a flash release versus traditional maceration expansion on the content of phenolic compounds (by LC–MS, UPLC–PDA), antioxidant activity (ABTS and FRAP), and other physical and chemical properties of wine from Pinot noir cultivar. The content of polyphenols in a must obtained by the flash release technology was higher than when using a traditional method. Flash release expansion improved the extraction of polyphenol compounds, especially anthocyanins, as well as color properties and antioxidant activity. However, during maturation and storage, the content of polyphenols significantly decreased, especially in the wine exposed to the flash release expansion. Despite lower total polyphenols content (1435 vs. 1686 mg/L in control), the final flash release wine contained more anthocyanins which resulted in approximately 2 times higher antioxidant activity. High correlation between antioxidant activity (r 2 = 0.84 and 0.79 for ABTS and FRAP assay, respectively) and the content of anthocyanins was determined in both types of examined wines. Changes in color were also observed in the must prepared by flash release versus traditional maceration. During fermentation, L* and a* parameters, the variables associated with more light and red color, increased, and during maturation, a rise in b* parameter, associated with yellow color, was observed.