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The objective of this study was to determine whether dietary tea tree oil (TTO) supplementation could effectively replace the antibiotics through modulating the antioxidant capacity and intestinal microbiota profile, and then decreasing the diarrhoea incidence and improving the growth performance of weaned pigs. A total of 216 weaned pigs with initial body weights (BW) of 9.19 ± 1.86 kg were randomly allocated to three dietary treatments in a completely randomised design. The dietary treatments included a corn-soybean meal basal diet (CON) without any antibiotics, and two experimental diets formulated by adding 75 mg/kg aureomycin (AGP) or 100 mg/kg TTO into the basal diet, respectively. Pigs fed the TTO diet showed greater gain to feed ratio (

Objective: The study was conducted to determine the effects of body weight (BW) and fiber sources on nutrient digestibility, fiber fermentation and short chain fatty acids (SCFA) concentration in different intestinal segments of growing pigs fed high-fiber diets.Methods: Nine barrows with initial BW of 25.17±0.73 kg and 9 barrows with initial BW of 63.47±2.18 kg were allotted to a duplicate 9×2 Youden Square design with 3 dietary treatments and 2 periods. The dietary treatments were formulated with 3 different high-fiber ingredients: corn bran, sugar beet pulp, and soybean hulls, respectively. Each diet was fed to 3 barrows with different stage of BW in each period.Results: There were no differences in the apparent ileal digestibility (AID) of most nutrients between pigs at different BW stages. Pigs at 60 kg had greater (p

Background Flammulina velutipes stem waste (FVS) is the by-product of mushroom industry. The objectives of this study were to determine the available energy and amino acid digestibility of FVS fed to pigs, and to evaluate the effects of dietary FVS inclusion on growth performance, biochemical profile of serum, fecal short chain fatty acid (SCFA) concentration, carcass traits, meat quality, intestinal morphology and microflora of pigs. In Exp. 1, twelve crossbred barrows with initial body weight (IBW) of 37.48 ± 4.31 kg were randomly allotted to 2 dietary treatments, including a corn basal diet and an experimental diet containing 24.35% FVS. In Exp. 2, twelve barrows fitted with an ileal T-cannula (IBW: 32.56 ± 1.67 kg) were randomly allotted to 2 dietary treatments, which included a N-free diet and an experimental diet containing 40.0% FVS. In Exp. 3, ninety growing pigs (IBW: 63.98 ± 6.89 kg) were allotted to 1 of 3 treatment diets for 63 d, including a basal diet and 2 experimental diets with 2.5% and 5% FVS, respectively. Results The digestible energy (DE) and metabolizable energy (ME) of FVS were 4.58 and 4.06 MJ/kg on dry matter basis, respectively, and the standardized ileal digestibility (SID) of indispensable AAs ranged from 17.50% to 59.47%. Pigs fed diets with 2.5% FVS showed no difference on average daily gain (ADG) and gain to feed ratio (G/F). Although dietary 5% FVS inclusion impaired apparent total tract digestibility (ATTD) of organic matter and gross energy, it elevated the SCFA concentration (P ≤ 0.04) in gut and antioxidant capacity in serum. In addition, dietary FVS inclusion depressed the backfat thickness (P = 0.03) in pigs. The longissimus dorsi muscle of pigs fed FVS revealed higher n-3 polyunsaturated fatty acid concentration and optimized fatty acid composition. Dietary 2.5% FVS inclusion also improved the intestinal development and health by increasing the villius height to crypt depth ratio (V/C) in jejunum (P Conclusions It is feasible to include moderate content of FVS as an unconventional fiber ingredient in diet of growing-finishing pigs.

This study was conducted to evaluate effects of Clostridium butyricum and corn bran supplementation on growth performance, nutrient digestibility, faecal volatile fatty acids, and microbiota in weaned pigs. Hundred and forty-four weaned pigs (7.01 ± 0.62 kg BW) were randomly assigned to 1 of 4 treatments, including the basal diet and 5% corn bran diet with or without Clostridium butyricum supplementation. The results showed the supplementation of 0.1% Clostridium butyricum in diets increased average daily gain (p

The objectives of this study were (a) to investigate the effect of body weight (BW) on available energy in fibre-rich diets containing two NDF levels; and (b) to evaluate the effect of fibre type and NDF level on AA digestibility. In Exp. 1, barrows (24 at 33.4 ± 1.1 kg; 24 at 86.8 ± 3.3 kg) were randomly allotted to 4 diets containing a high protein basal (HPB), a low protein basal (LPB), 70% HPB + 30% wheat bran (WB) (HPB-WB) and 70% LPB + 30% sunflower meal (SFM) (LPB-SFM). In Exp. 2, barrows (24 at 33.5 ± 1.8 kg; 24 at 87.0 ± 3.8 kg) were randomly allotted to 4 diets containing no SFM with 12% NDF (SFM0LF), no SFM with 16% NDF (SFM0HF), 24% SFM with 12% NDF (SFM24LF) and 24% SFM with 16% NDF (SFM24HF). In Exp. 3, twelve barrows (35.5 ± 3.4 kg) fitted with T-cannulas were allotted to two 6 × 4 Youden square design with 6 diets (4 same diets as in Exp. 2 and 2 nitrogen-free diets). The concentration of DE, ME and ATTD of GE, CP, NDF and ADF in diets was greater (p < .01) when fed to 90 kg pigs compared with 30 kg pigs. The ME in ingredients was not affected by BW. The ATTD of NDF was negatively correlated with the CF (r = -.98; p < .01), ADF (r = -.99; p < .01) and ADL content (r = -.96; p < .01). The ME of diets was negatively correlated with the ATTD of CF (r = -.98; p < .01). The addition of SFM increased the standardized ileal digestibility (SID) of Met (p < .05). In conclusion, diets rich in fibre had different nutritional values at different pig weight stages. The AA digestibility depends mainly on chemical composition of diets.

This study was conducted to determine the digestible energy (DE) and metabolizable energy (ME) values and apparent total tract digestibility (ATTD) of nutrients in four fibrous ingredients [corn distillers’ dried grains with solubles (DDGS), soybean hull, wheat bran, and corn bran] fed to barrows at three different growth stages. Thirty growing barrows, 30 finishing barrows, and 30 fattening barrows (initial body weights of 29.04, 58.57, and 105.65 kg, respectively) were individually housed in metabolism crates and allotted to one of four test diets or a basal corn–soybean meal diet in a 3 × 5 factorial design. Fecal and urine samples were collected for 5 d after a 12 d adaption period. The DE and ME values and ATTD of gross energy (GE), organic matter (OM), crude protein, and acid detergent fiber (ADF) in wheat bran, as well as the ATTD of GE, OM, neutral detergent fiber (NDF), and ADF in corn DDGS, and ATTD of NDF and ADF in soybean hull, were greater (P

This study aimed to explore effects of different fiber-rich foods on bacterial community and their metabolites in pig model. Thirty-six pigs were allotted to 6 dietary treatments including corn bran (CB), wheat bran (WB), oat bran (OB), soybean hulls (SH), sugar beet pulp (SBP) and rice bran (RB). The results demonstrated that lactate was mainly produced in the foregut, whereas propionate and butyrate were generated in the hindgut of pig. The ileal acetate and fecal butyrate production were positively correlated with the dietary acid detergent fiber and cellulose content, respectively. Different dietary fiber supplementation altered Filifactor and Intestinibacter abundances in ileal digesta and Ruminococcus_1 and Lachnoclostridium populations in feces of pigs. The differences in fiber fermentation and SCFAs production in ileal and fecal samples were associated with microbial compositions. Overall, this study would help to better understand how to promote the gut health through different fiber-rich foods intake in humans.

This study was conducted to determine the effect of different growing stages on digestible energy (DE) and metabolizable energy (ME) in soybean meal (SBM), cottonseed meal (CSM), rapeseed meal (RSM), corn gluten meal (CGM), and corn distillers dried grains with solubles (DDGS) fed to crossbred barrows. Thirty-six growing barrows (GS 1: 29.1 ± 2.85 kg BW), 36 growing barrows (GS 2: 59.2 ± 4.91 kg BW), and 36 finishing barrows (GS 2: 105.4 ± 6.91 kg BW) were allotted to 6 diets in a completely randomized design with 6 replicated pigs per diet per growth stage or BW. Faecal and urine samples were collected for 5 days after a 12-day adaption period. The results showed that the concentration of DE in corn for pigs at GS 3 was greater (P

This study was conducted to evaluate the effect of dietary Chinese herb medicine extracted from Dandelion root on growth performance, serum immune markers, faecal bacterial community and their metabolites in weaned pigs. The results showed that dietary Dandelion root extract (CHE) supplementation decreased (P

This study was conducted to determine the DE, ME, and apparent (AID) and standardized ileal digestibility (SID) of AA in different wheat cultivars fed to growing pigs. In Exp. 1, twelve crossbred growing barrows were allotted to a replicated 6 × 6 Latin square design to determine the DE and ME contents of 12 different wheat cultivars. In Exp. 2, twelve growing barrows with a simple T-cannula were used to determine the AID and SID of AA in 10 different wheat cultivars. Pigs were randomly allotted to a replicated 6 × 6 Latin square design and fed one nitrogen-free diet and 10 different cultivars of wheat diets. Among different wheat cultivars, the concentrations of GE, CP, NDF, ADF, and starch ranged from 4,385 to 4,458 kcal/kg, 12.95% to 18.14%, 9.16% to 13.89%, 1.93% to 2.92% and 60.81% to 70.77%, respectively (DM basis). There were significant differences (P < 0.05) in the DE, ME values, and the ATTD of GE in 12 different cultivars of wheat in Exp. 1. The DE and ME contents and the ATTD of GE ranged (P < 0.05) from 3,922 to 4,067 kcal/kg DM, 3,759 to 3,941 kcal/kg DM and 88.14% to 90.31%, respectively. The best prediction equations of DE and ME for different cultivars of wheat cultivars were DE = -2,738 - (40.8 × % ADF) + (1.7 × GE) - (51.5 × % Xylans) - (95.7 × % Ash) + (22.3 × % EE), R2 = 0.98, and ME = -2,990 + (1.7 × GE) - (50.2 × % Xylans) - (87.6 × % Ash), R2 = 0.88, respectively. There were also differences (P < 0.05) in the AID and SID of CP and indispensable AA, expect for His, in 10 wheat cultivars in Exp. 2. The AID and SID of CP, Lys, and Met ranged (P < 0.05) from 82.02% to 89.46%, 74.13% to 84.73%, 87.35% to 92.49% and 87.56% to 94.04%, 80.56% to 89.89%, 89.56% to 94.45%, respectively. In conclusion, the chemical composition, energy contents, and most AA digestibility in different cultivars of wheat varied widely.

Lactose plays a crucial role in the growth performance of pigs at weaning because it is a palatable and easily digestible energy source that eases the transition from milk to solid feed. However, the digestibility of lactose declines after weaning due to a reduction in endogenous lactase activity in piglets. As a result, some lactose may be fermented in the gastrointestinal tract of pigs. Fermentation of lactose by intestinal microbiota yields lactic acid and volatile fatty acids, which may positively regulate the intestinal environment and microbiome, resulting in improved gastrointestinal health of weanling pigs. We hypothesize that the prebiotic effect of lactose may play a larger role in weanling pig nutrition as the global feed industry strives to reduce antibiotic usage and pharmacological levels of zinc oxide and supra-nutritional levels of copper. Evidence presented in this review indicates that high dietary lactose improves growth performance of piglets, as well as the growth of beneficial bacteria, particularly Lactobacillus, with the positive effects being more pronounced in the first 2 weeks after weaning. However, the risk of post-weaning diarrhea may increase as pigs get older due to reduced lactase activity, high dietary lactose concentrations, and larger feed intakes, all of which may lead to excessive lactose fermentation in the intestine of the pig. Therefore, dietary lactose levels exert different effects on growth performance and gastrointestinal physiological functions in different feeding phases of weanling pigs. However, no formal recommendation of lactose for weanling pigs has been reported. A meta-analysis approach was used to determine that diets fed to swine should include 20%, 15%, and 0 lactose from d 0–7, d 7–14, and d 14–35 post-weaning, respectively. However, sustainable swine production demands that economics must also be taken into account as lactose and lactose containing ingredients are expensive. Therefore, alternatives to lactose, so called “lactose equivalents” have also been studied in an effort to decrease feed cost while maintaining piglet performance with lower dietary lactose inclusions. In summary, the present review investigated dose-response effects of dietary lactose supplementation to exert positive responses and begin to elucidate its mechanisms of action in post-weaning pig diets. The results may help to replace some or all lactose in the diet of weanling pigs, while improving production economics given the high cost of lactose and availability in some swine production markets.

The objective of this study was to quantify the relative bioavailability of microencapsulated vitamins A and E in nursery pigs and compare the effects of vitamin forms and vitamin levels on the plasma vitamin content and growth performance of weaned piglets. In experiment (Exp.) 1, 12 nursery pigs (fitted with jugular catheters) were supplied at 0 h with non-microencapsulated or microencapsulated vitamin A and E. Blood samples were collected at 1, 3, 6, 9, 12, 16, 18, 21, 24, 27, 30, 36, 48, and 72 h after feeding to compare the bioavailability of oral vitamins A and E. In Exp. 2, a total of 216 crossbred weaned piglets were assigned to six treatments. This experiment was a 2 &times, 3 factorial arrangement, with two factors for vitamin forms (non-microencapsulated and microencapsulated) and three factors for vitamin levels (the National Research Council level of vitamins, 75% commercial recommendations of vitamins (CRV) level, and a 100% CVR level). In Exp. 1, the relative bioavailability of microencapsulated vitamin E was significantly greater than that of non-microencapsulated vitamin E. In Exp. 2, the pigs fed diets containing 75% or 100% CRV levels of vitamins increased their growth performance and plasma vitamin concentrations compared to the control group. In conclusion, microencapsulation can improve the bioavailability of vitamins, and supplementation with high levels of vitamins was able to improve the growth performance of the piglets.

The study was conducted to determine effects of a complex of fibre-degrading enzymes (xylanase, cellulase and β-glucanase) on nutrient digestibility, fibre fermentation and concentrations of short chain fatty acids (SCFA) at different parts of digestive tract in pigs fed different fibre-rich ingredients. A total of 36 barrows fitted with T-cannulas in the distal ileum (initial body weight of 41.1 ± 2.7 kg) were randomly allotted to six dietary treatments with three different high-fibre diets including maize bran (MB), sugar beet pulp (SBP) and soybean hulls (SH) with or without supplementation of fibre-degrading enzymes. Enzyme supplementation improved (

This study was conducted to evaluate the effects of dietary FVS supplementation on growth performance, nutrient digestibility, biochemical profile of serum and fecal short chain fatty acids (SCFAs) production in weaned piglets. In Exp.1, 150 weaned pigs (initial body weight: 6.89 &plusmn, 1.17 kg) were allotted to five dietary treatments. The treatment diets included a basal diet and four experimental diets supplemented with 2.5%, 5.0%, 7.5% and 10.0% FVS respectively. The animal trial lasted for 28 days. In Exp.2, 72 piglets (initial body weight: 8.20 &plusmn, 1.67 kg) were allotted to three dietary treatments. The treatment diets included a basal diet and two experimental diets supplemented with 1.5% and 3.0% FVS, respectively. The animal trial lasted for 56 days. The results showed that pigs fed dietary FVS with 3% or lower inclusion levels had no significant difference (p &gt, 0.10) on growth performance compared with pigs fed the control diet during day 1&ndash, 28 and day 1&ndash, 56. Dietary FVS supplementation decreased the apparent total tract digestibility (ATTD) of nutrients on day 28, day 35 and day 56, but no significant changes (p &gt, 0.05) of nutrient digestibility were observed on day 14. Although piglets fed diets with higher levels of FVS showed impaired growth performance and ATTD of nutrients, dietary FVS supplementation improved the fecal SCFA production, antioxidant capacity, interleukin-2 and growth hormone levels in serum, and reduced the harmful low-density lipoprotein levels in serum on day 56. In conclusion, as a promising alternative fibrous ingredient, FVS could be supplemented in diets of weaned piglets with a proportion under 3%.

In the present study, the pathogenicity of fowl adenovirus (FAdV) serotype 4 strain HB1501 was systematically determined by using different doses and routes of inoculation in chickens and assessed the immune efficacy induced by fowl adenovirus serotype 4 (FAdV-4) strain SB15 in a vaccination trial. The results revealed that strain HB1501 was highly virulent in chickens, inflicting 30 to 100% mortality when administered by intramuscular and intravenous routes, and its pathogenicity was influenced markedly by the inoculation route. The inactivated oil-emulsion FAdV-4 vaccine derived from strain SB15 completely protected specific pathogen-free chickens against morbidity and mortality following infection with the virulent FAdV-4 strain HB1501. Importantly, no obvious clinical signs or gross lesions were observed and high antibody levels against FAdV were measured in the SB15-vaccinated chickens, indicating the potential of this strain to become an alternative vaccine candidate. This study provides detailed information on the pathogenicity of FAdV-4, which should be of great value to the control of hepatitis-hydropericardium syndrome.

The objectives of this study were to determine: 1) the effects of konjac flour residues and ramie on digestible energy (DE), metabolizable energy (ME) and apparent total tract digestibility (ATTD) of nutrients in diets fed to growing pigs, 2) the DE and ME contents of konjac flour residues and ramie. Thirty barrows were allotted to 1 of 5 treatments with 6 replicates per treatment. The 5 diets include a corn-soybean meal basal diet (CTL), konjac flour residues diets containing 15% konjac flour residues (LK) or 30% konjac flour residues (HK), and ramie diets containing 15% ramie (LR) or 30% ramie (HR). The experiment lasted 19 days, including 7 days for cage adaptation, 7 days for diet adaptation, and 5 days for total feces and urine collection. The energy values and ATTD of nutrients in each diet were determined, and DE and ME contents of konjac flour residues and ramie were calculated. The results showed that consumption of konjac flour residues significantly increased (P

The objective of this study was to evaluate the differences in nutrient digestibility between Duroc × Landrace × Yorkshire (DLY) and Duroc × Berkshire × Jiaxing (DBJ) growing pigs when fed conventional ingredients. A number of 14 DLY and 14 DBJ growing barrows, surgically fitted with T-cannula in the distal ileum, were allotted to a 14 × 3 Youden Square Design with 7 experimental diets and 3 periods, respectively. Each diet was fed to 2 pigs in each breed, and each period lasted for 15 days, including 10 days for diet adaptation, 3 days for fecal collection and 2 days for digesta collection. The apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of indispensable amino acids (IAAs) and the apparent total tract digestibility (ATTD) of chemical constituents in corn and barley were calculated using the direct method, while those in wheat middlings, rice bran and alfalfa meal were calculated using the difference method. Hindgut disappearance was calculated as the difference between the AID value and ATTD value. Results showed that the AID of ether extract (EE) and neutral detergent fiber (NDF) in corn and barley were greater (P

OBJECTIVE This experiment was conducted to determine the differences in the apparent ileal (AID) and total tract digestibility (ATTD) of nutrients and indispensable amino acids (IAA) in high-fiber diets with wheat middlings, rice bran or alfalfa meal fed to Duroc×(Landrace× Yorkshire) (DLY) and Duroc× (Berkshire×Jiaxing) (DBJ) growing barrows. METHODS Eighteen DLY and 18 DBJ growing barrows were randomly allotted to a 2×3 factorial arrangement involving 2 crossbreeds and 3 high-fiber diets. The experiment lasted 15 d with 10 d for diets adaptation, 3 d for feces collection and 2 d for digesta collection. Three diets were based on corn and soybean meal with 25% wheat middlings, rice bran and alfalfa meal respectively. RESULTS DBJ had a greater (p

Abstrast The objective of this study was to evaluate the effects of wheat bran (WB) levels on the apparent total tract digestibility (ATTD) of dietary nutrient in growing pigs, and to test the hypothesis that dietary fiber could alter microbiota community and increase the synthesis of short-chain fatty acid (SCFAs), thus improving the growth performance of nursery pigs. In Exp. 1, 30 barrows with initial body weight (BW) of 40.5 ± 3.0 kg were allotted to 5 treatments in a completely randomized design. The five treatment diets included 1 control diet and 4 test diets with the WB inclusion level of 5, 15, 25 and 35%, respectively. The concentration of DE and ATTD of nutrients in diets decreased linearly (P

The objective of this study was to compare the effect of source (corn DDGS, rice bran, or soybean) or form of oil (extracted or intact) on apparent total tract digestibility (ATTD) and true total tract digestibility (TTTD) of acid-hydrolyzed ether extract (AEE) and fatty acids. The study determined and compared the ATTD or TTTD of AEE and fatty acids in extracted corn oil, rice oil, and soybean oil with intact oil in corn DDGS, full-fat rice bran, and full-fat soybean. Seventy-eight barrows (initial BW = 47.2 ± 3.9 kg; Duroc × Landrace × Yorkshire) were allotted to 1 of 13 dietary treatments in a randomized complete block design with 6 barrows in each dietary treatment. The 13 experimental diets included 1 cornstarch-soybean meal basal diet (AEE, 0.56%) and 3 diets containing 6% extracted oils (corn oil, rice oil, and soybean oil) and 9 diets supplemented with 3 levels of corn DDGS (17%, 34%, and 51%), full-fat rice bran (14%, 28%, and 42%), and full-fat soybean (12%, 24%, and 36%). These diets provided about 2%, 4%, and 6% intact oil, respectively. The barrows were housed in individual metabolism crates and were fed the assigned test diets at 4% of initial BW per day. A 5-d total collection of feces followed a 7-d diet adaptation period. The ATTD of AEE were calculated for each diet. The endogenous flow of AEE associated with each ingredient and values for TTTD were calculated using regression methods. The ATTD of AEE were greater ( < 0.05) for extracted oil than for intact oil. Compared to extracted oil, intact oil had lower ( < 0.01) ATTD of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3). However, a source by form interaction ( < 0.01) was observed for ATTD of C18:0, C18:2, and C18:3. The ATTD of C18:0, C18:2, and C18:3 in extracted corn and rice oil were greater compared with intact corn DDGS and rice oil ( < 0.05), while there was no significant difference for the ATTD of C18:0, C18:2, and C18:3 between extracted soybean oil and intact oil in full-fat soybean. In conclusion, lipids that are extracted have a greater digestibility compared with intact lipids, and this is especially true regarding saturated fatty acids. The ATTD of AEE in 2 forms of rice oil (intact oil and extracted oil) was less than the values in corn oil and soybean oil. The TTTD of AEE in corn DDGS and full-fat soybeans were greater than in full-fat rice bran.

OBJECTIVE This experiment was to evaluate the effect of different inclusion levels and adaptation periods on digestible energy (DE) and the apparent total tract digestibility (ATTD) of chemical constituents in diets supplemented with wheat bran. METHODS Thirty-six crossbred barrows with an initial body weight of 85.0±2.1 kg were allotted to 6 diets in a completely randomized block design with 6 pigs per diet. Diets included a corn-soybean basal diet and 5 additional diets which were formulated by replacing corn and soybean meal in control diet with 15%, 25%, 35%, 45%, or 55% wheat bran. The experiment lasted for 34 d, and feces were collected from d 8 to 13, 15 to 20, 22 to 27, and 29 to 34 respectively. RESULTS The results showed no interaction effects between inclusion level and adaptation period on the concentration of DE and the ATTD of gross energy (GE) and crude protein (CP) in wheat bran. The DE value and ATTD of GE in wheat bran decreased (p

Corn bran (CB) has been used as an ingredient for pigs, but the underlying mechanisms that improve gut health is less clear. This study was conducted to investigate effects of dietary CB on growth performance, nutrient digestibility, plasma indices related to gut hormones and immunity, gut microbiota composition, and fermentation products in weaned pigs. A total of 60 weaned pigs were allocated to two dietary treatments, and piglets in each group received control (CON) diet or 5% CB diet for 28 days. Growth performance, nutrient digestibility, indices of gut hormones and immunity in plasma were evaluated. Microbiota composition in feces was determined using 16S rRNA amplicon sequencing, and fermentation products were measured by high-performance ion chromatography. The results showed that dietary CB did not affect growth performance, nutrient digestibility, gut hormones, or fermentation products in the trial (P > 0.05). There was an increased response to CB inclusion on interleukin-10 production (P < 0.05). On day 28, piglets fed dietary CB had a higher shannon index (P < 0.05). The population of the Firmicutes in CB treatment were decreased (P < 0.05), while the percentage of the Bacteroidetes were increased (P < 0.05). In particular, the populations of Eubacterium corprostanoligenes, Pevotella, and Fibrobacter related to polysaccharide fermentation of cereal bran were increased (P < 0.05). In conclusion, a post-weaning diet containing 5% CB increased intestinal microbial diversity, especially higher richness of fibrolytic bacteria, and promoted anti-inflammatory response to some extent in piglets, these changes should facilitate the adaptation of the digestive system of piglets in the subsequent growing phases.

The study investigated the impact of dietary fibers on the performance, fecal short-chain fatty acids, nutrient digestibility, and bacterial community in weaned piglets with the control group (CON) and dietary supplementation of 5% corn bran (CB), 5% wheat bran (WB), or 5% soybean hulls (SB). The piglets in CB and WB groups showed greater weight gain and feed efficiency ( p0.05) in comparison to piglets in CON and SB groups. Fecal samples from piglets in CB, SB, and WB groups contained greater ( p0.05) butyrate levels than fecal samples from piglets in the CON group. The fecal samples from piglets in CB or WB groups contained greater ( p0.05) abundances of Actinobacteria and Firmicutes or Fibrobacteres than the fecal sample from piglets in the CON group, which could promote fiber degradation and the production of butyrate. In summary, dietary CB or WB may enhance the growth performance of weaned piglets via altering gut microbiota and improving butyrate production, which shed light on the mechanism of dietary fiber in improving gut health.

Objective An experiment was conducted to evaluate effects of inclusion level of palm kernel meal (PKM) and adaptation duration on the digestible energy (DE) and apparent total tract digestibility (ATTD) of chemical constituents in diets fed to growing-finishing pigs. Methods Thirty crossbred barrows (Duroc×Landrace×Large White) with an average initial body weight of 85.0±2.1 kg were fed 5 diets in a completely randomized design. The diets included a corn-soybean meal basal diet and 4 additional diets in which corn and soybean meal were partly replaced by 10%, 20%, 30%, or 40% PKM. After 7 d of adaptation to the experimental diets, feces were collected from d 8 to 12, d 15 to 19, d 22 to 26, and d 29 to 33, respectively. Results The DE and ATTD of gross energy (GE), dry matter (DM), ash, organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude protein (CP) in diets decreased linearly as the dietary PKM increased within each adaptation duration (p< 0.01). Diet containing 19.5% PKM had less DE value and ATTD of all detected items compared with other diets when fed to pigs for 14 days (p 0.05). Considering a stable determination, 21 days of adaptation to a diet containing 19.5% PKM is needed in pigs and a longer adaptation time is recommended as dietary PKM increases.

This study was conducted to evaluate effects of inclusion level and supplementary amino acids (AAs) on digestible energy (DE) and metabolizable energy (ME) values of soybean meal (SBM) fed to growing pigs, determined by difference and regression methods. Sixty pigs were fed 10 diets according to a 5 × 2 factorial arrangement. Two control diets contained 97.34% corn without supplementary AAs or 95.61% corn with supplementary AAs. Eight diets were formulated by replacing corn and AAs in control diets with 8%, 15%, 25% and 31% SBM. There was no difference in DE and ME values of SBM determined by difference method as inclusion level of SBM increased or crystalline AAs were added. No difference was observed in DE and ME values of SBM determined by the two methods in diets without supplementary AAs, but the values determined by the difference method were greater (P

Background Two experiments were conducted to estimate the net energy (NE) of corn, soybean meal, expeller-pressed rapeseed meal (EP-RSM) and solvent-extracted rapeseed meal (SE-RSM) using indirect calorimetry and to validate the NE of these four ingredients using pig growth performance. Methods In Exp.1, 24 barrows (initial BW = 36.4 ± 1.6 kg) were allotted to 1 of 4 diets which included a corn basal diet, a corn-soybean meal basal diet and two rapeseed meal diets containing 20% EP-RSM (9.5% ether extract) or SE-RSM (1.1% ether extract) substituted for corn and soybean meal. The design allowed the calculation of NE values of corn, soybean meal and rapeseed meals according to the difference method. In Exp.2, 175 growing pigs (initial BW = 36.0 ± 5.2 kg) were fed 1 of 5 diets for 28 d, with five pigs per pen and seven replications (pens) per treatment in order to validate the measured energy values. Diets were a corn-soybean meal diet and four diets including 10% or 20% EP-RSM and 10% or 20% SE-RSM. Results The NE of corn, soybean meal, EP-RSM and SE-RSM were 12.46, 11.34, 11.71 and 8.83 MJ/kg DM, respectively. The NE to ME ratio of corn (78%) was similar to tabular values, however, the NE to ME ratios of soybean meal (70%) and rapeseed meal (76%) were greater than tabular values. The greater NE value in EP-RSM than in SE-RSM is consistent with its higher EE content. Increasing EP-RSM or SE-RSM did not affect the growth performance of pigs and the caloric efficiency of NE was comparable for all diets. Conclusions The NE of EP-RSM was similar to soybean meal, and both were greater than SE-RSM. The DE, ME and NE values measured in Exp.1 are confirmed by results of Exp. 2 with comparable caloric efficiencies of DE, ME or NE for all diets.

OBJECTIVE The objective of this study was to determine the effect of inclusion level on the digestible energy (DE), metabolizable energy (ME), and total tract digestibility of acid-hydrolyzed ether extract (AEE) of cottonseed oil when fed to growing pigs. METHODS Forty-two barrows (initial body weight = 35.51±2.01 kg) were randomly allotted to a completely randomized design with a corn-soybean meal basal diet, five levels of cottonseed oil (2%, 4%, 6%, 8%, and 10%) and a 10% soybean oil diet. Each diet was replicated six times with one pig per replicate. The experiment lasted 19 days, 7 d for cage adaptation, 7 d for diets adaptation and last 5 d for feces and urine collection. The energy values and apparent total tract digestibility (ATTD) of cottonseed oil and soybean oil were calculated by the difference method, and regression equations were established to predict the energy values of cottonseed oil. The apparent digested fat of the entire intestinal tract was also regressed against dietary fat intake to determine the true total tract digestibility (TTTD) and endogenous loss of fat for cottonseed oil. RESULTS The results showed that the DE and ME contents of cottonseed oil were not different as the inclusion level increased. The DE and ME values determined by the regression equation were 36.28 MJ/kg and 34.96 MJ/kg, respectively, and the values were similar to the mean DE and ME values calculated by the difference method (36.18 and 35.56 MJ/kg, respectively). The ATTD of cottonseed oil was also not affected by the inclusion level of cottonseed oil, and the TTTD and EFL determined by the regression method were 92.40% and 13.83 g/kg of dry matter intake for corn-soybean basal diet. The DE, ME, and ATTD of AEE in soybean oil determined by the difference method were 35.70 MJ/kg, 35.20 MJ/kg and 92.31%, respectively. There were no differences in the DE, ME, and ATTD between cottonseed oil and soybean oil, although the ratio of unsaturated to saturated fatty acids for soybean oil was higher than for cottonseed oil. CONCLUSION The DE, ME, and ATTD values of cottonseed oil were not affected by its dietary inclusion level. The energy values of cottonseed oil determined by the difference and regression methods were similar. Furthermore, the ratio of unsaturated to saturated fatty acid for oils was not the decisive factor to influence the energy values and ATTD of oils.