1. The EmsB tandemly repeated multilocus microsatellite: a new tool to investigate genetic diversity of Echinococcus granulosus sensu lato
- Author
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Bruno Gottstein, Renaud Piarroux, M. C. Benchikh-Elfegoun, I. Colovic, S. Ma, Karen Luisa Haag, Jenny Knapp, S. Maillard, Institut de l'Ouest : Droit et Europe ( IODE ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Centre National de la Recherche Scientifique ( CNRS ), Institute of Parasitology, University of Bern, Laboratoire Evolution, Génomes et Spéciation ( LEGS ), Centre National de la Recherche Scientifique ( CNRS ), The Institute of Automation of the Chinese Academy of Sciences ( CASIA ), Chinese Academy of Sciences [Beijing] ( CAS ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques ( IP-TPT ), Institut de Recherche pour le Développement ( IRD ) -Aix Marseille Université ( AMU ) -Assistance Publique - Hôpitaux de Marseille ( APHM ) -Service de Santé des Armées-Université de Montpellier ( UM ), Millon, Laurence, Institut de l'Ouest : Droit et Europe (IODE), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Evolution, Génomes et Spéciation (LEGS), Centre National de la Recherche Scientifique (CNRS), The Institute of Automation of the Chinese Academy of Sciences (CASIA), Chinese Academy of Sciences [Beijing] (CAS), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques (IP-TPT), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Service de Santé des Armées, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), and Service de Santé des Armées-Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)
- Subjects
MESH : Algeria ,MESH : Genotype ,MESH: DNA, Helminth ,MESH : Romania ,MESH : Microsatellite Repeats ,MESH : Echinococcosis ,MESH: Echinococcus granulosus ,030308 mycology & parasitology ,MESH: Genotype ,MESH: Dogs ,MESH : Dogs ,MESH : Mauritania ,MESH : Cattle ,MESH : DNA, Helminth ,Genotype ,Cluster Analysis ,MESH: Animals ,MESH: Genetic Variation ,Echinococcus granulosus ,Genetics ,0303 health sciences ,biology ,Mauritania ,MESH: Camels ,DNA, Helminth ,Echinococcosis ,MESH: China ,3. Good health ,MESH: Cattle ,Microsatellite ,MESH : Sensitivity and Specificity ,MESH: Algeria ,Serbia ,Brazil ,MESH: Mauritania ,Microbiology (medical) ,China ,Camelus ,MESH: Sheep ,MESH : Sheep ,Sensitivity and Specificity ,MESH: Romania ,[ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology, environment/Health ,03 medical and health sciences ,MESH: Echinococcosis ,Dogs ,Sensu ,MESH : Genetic Variation ,Genetic variation ,parasitic diseases ,medicine ,Animals ,Humans ,[SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health ,MESH : Brazil ,MESH : China ,MESH : Cluster Analysis ,Genotyping ,MESH : Serbia ,030304 developmental biology ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,Genetic diversity ,Sheep ,MESH: Humans ,MESH : Camels ,Romania ,MESH : Echinococcus granulosus ,MESH : Humans ,Genetic Variation ,medicine.disease ,biology.organism_classification ,MESH: Cluster Analysis ,MESH: Sensitivity and Specificity ,MESH: Serbia ,Evolutionary biology ,Algeria ,Cattle ,Parasitology ,MESH: Microsatellite Repeats ,MESH : Animals ,MESH: Brazil ,Microsatellite Repeats - Abstract
Cystic echinococcosis (CE) is a widespread and severe zoonotic disease caused by infection with the larval stage of the eucestode Echinococcus granulosus sensu lato. The polymorphism exhibited by nuclear and mitochondrial markers conventionally used for the genotyping of different parasite species and strains does not reach the level necessary for the identification of genetic variants linked to restricted geographical areas. EmsB is a tandemly repeated multilocus microsatellite that proved its usefulness for the study of genetic polymorphisms within the species E. multilocularis , the causative agent of alveolar echinococcosis. In the present study, EmsB was used to characterize E. granulosus sensu lato samples collected from different host species (sheep, cattle, dromedaries, dogs, and human patients) originating from six different countries (Algeria, Mauritania, Romania, Serbia, Brazil, and the People's Republic of China). The conventional mitochondrial cox1 and nad1 markers identified genotypes G1, G3, G5, G6, and G7, which are clustered into three groups corresponding to the species E. granulosus sensu stricto, E. ortleppi , and E. canadensis . With the same samples, EmsB provided a higher degree of genetic discrimination and identified variations that correlated with the relatively small-scale geographic origins of the samples. In addition, one of the Brazilian single hydatid cysts presented a hybrid genotypic profile that suggested genetic exchanges between E. granulosus sensu stricto and E. ortleppi . In summary, the EmsB microsatellite exhibits an interesting potential for the elaboration of a detailed map of the distribution of genetic variants and therefore for the determination and tracking of the source of CE.
- Published
- 2009